Transport of ions and biomolecules through single asymmetric nanopores in polymer films

Schiedt, B.; Healy, Ken; Morrison, Alan P.; Neumann, R.; Siwy, Zuzanna S.

doi:10.1016/j.nimb.2005.03.265

Cited by 94 publications

(107 citation statements)

References 16 publications

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“…In agreement with the I-C data and theoretical model presented above, the pore conductance overproportionally increases with increasing electrolyte concentration at low concentrations. Our results are in close agreement with the data reported by other researchers fabricating cylindrical and conical nanopores in surface charged membranes [28,29].…”

Section: Impact Of the Electrolyte Concentration Onsupporting

confidence: 93%

“…Applied voltages range from +10 mV to +100 mV. Although standard electrolyte concentrations for biological experiments only range down to 0.1 M, we have still measured the current through our nano pore for the two additional electrolyte concentrations of 0.02 and 0.5 M. In accordance to the previous studies [29], we also identified that the dependence of the ionic current through a single artificial nano pore on the electrolyte concentrations deviates to a curve with a steeper slope at all the voltages applied as the electrolyte concentration is lowered to values below 0.1 M (Figure 4).…”

Section: Impact Of the Electrolyte Concentration Onsupporting

confidence: 83%

“…This can be explained by a model focusing on electrostatic interactions between ions passing through the pore and surface charge distribution on the pore walls [29]. Almost all microfabrication processes and their accompanying pore development steps leave artificial nano pores in either polymer membranes or silicon-based substrates with a net negative charge on their surface and walls [18].…”

Section: Current-voltage (I-v) Characteristicsmentioning

confidence: 99%

“…The negative surface charge on the PMMA membrane due to the formation of carboxylate end groups attracts K + ions from the electrolyte to the pore surface and walls, forming an electric double-layer [4,29]. This double layer reduces the effective charges/potential available for the electrochemical transport mechanism.…”

Section: Impact Of the Electrolyte Concentration Onmentioning

confidence: 99%

See 3 more Smart Citations

PMMA Polymer Membrane-Based Single Cylindrical Submicron Pores: Electrical Characterization and Investigation of Their Applicability in Resistive-Pulse Biomolecule Detection

Achenbach¹,

Hashemi²,

Moazed³

et al. 2012

AJAC

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Single cylindrical submicron pores in PMMA polymer membranes are micropatterned by electron beam lithography and integrated into all PMMA-based electrophoretic flow detector systems. Pore dimensions are 450 nm in diameter and 1 μm in length. The pores are electrically characterized in aqueous KCl electrolyte, exhibiting a stable time-independent ionic current through the pore with a noise level of less than 1% of the open-pore current. The current-voltage curves are linear and scale with electrolyte concentration. The negative surface charge of the membrane over-proportionally decreases pore conductance at low electrolyte concentrations (≤0.1 M) that are still beyond those typically applied in biological experiments. Pores do not exhibit rectification of current flowing through them, allowing for operation with either polarity. To allow for detection of yet much smaller particles, the described PMMA-based system also was successfully equipped with pores of 1.5 nm instead of 450 nm in diameter. This was achieved by introducing naturally occurring biological protein pores of α-hemolysin on a lipid bi-layer into the pre-patterned PMMA membrane of an assembled PMMA-based electrophoretic flow detector system. Characteristics of translocation events of single-stranded linear plasmid DNA molecules through the pores were recorded, and ionic current deductions during biomolecule translocation were clear and distinguished. Based on the presented submicron scale open pore ionic current transport properties, as well as the observed passage of DNA molecules through protein pores inserted into PMMA membranes, our current research proposes that all PMMA electrophoretic flow detectors exhibit an excellent potential for future use as biomedical resistive-pulse sensors, as long as pore dimensions match those of biomolecules to be detected.

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Section: Impact Of the Electrolyte Concentration Onsupporting

confidence: 93%

Section: Impact Of the Electrolyte Concentration Onsupporting

confidence: 83%

Section: Current-voltage (I-v) Characteristicsmentioning

confidence: 99%

Section: Impact Of the Electrolyte Concentration Onmentioning

confidence: 99%

See 2 more Smart Citations

PMMA Polymer Membrane-Based Single Cylindrical Submicron Pores: Electrical Characterization and Investigation of Their Applicability in Resistive-Pulse Biomolecule Detection

Achenbach¹,

Hashemi²,

Moazed³

et al. 2012

AJAC

View full text Add to dashboard Cite

show abstract

“…Various groups have started to use single conical pores with very small diameters (Siwy et al, 2003(Siwy et al, , 2002 as sensors for single molecules, e.g. desoxyribonucleic acid (DNA) (Heins et al, 2005;Mara et al, 2004;Schiedt et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Cylindrical nanochannels in ion-track polycarbonate membranes studied by small-angle X-ray scattering

et al. 2007

Self Cite

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Different types of polycarbonate foils were irradiated with 1.4 GeV Xe ions, ultra-violet (UV) treated and subsequently etched, creating cylindrical pores of high aspect ratio. The pores are perfectly well aligned and represent excellent objects for small-angle X-ray scattering. Two-dimensional scattering spectra exhibit highly anisotropic patterns with clear presentation of numerous oscillations of the Bessel function, the radial part of the scattering function. Modelling the pores as parallel cylinders allows us to deduce the pore radius and the radius dispersion as a function of UV treatment, etching time and fluence. It is demonstrated that the UV treatment has a beneficial influence on the poresize distribution, in particular for small pores.

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Electrokinetically Controlled Asymmetric Ion Transport through 1D/2D Nanofluidic Heterojunctions

Cao

et al. 2019

Adv Materials Technologies

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Supported 2D layered materials are widely used in gas separation, water treatment, energy conversion, etc. In conventional viewpoint, the atop 2D membrane functions as an active layer for mass or charge separation in vapor or liquid phase, while the substrate membrane, containing aligned or tortuous 1D micro or nanoscaled fluidic channels, functions as a mechanical support. However, asymmetric transport property induced by the mixed‐dimensional composite structure is an equally very important, yet long‐overlooked element that endows new features to the membrane‐scale nanofluidic systems and contributes to the promotion of overall performance. Here, reported are asymmetric ion transport properties through 1D/2D nanofluidic heterojunction membrane (NFHM) under three different types of electrokinetic driving force. The 1D/2D NFHM comprises of self‐assembled graphene oxide multi‐layers (2DM, negatively charged) supported on a polydopamine‐coated 1D nanopore array (1DM, ampholytic). Intriguingly, it is found that the preferential direction for electric‐field‐driven ionic transport is from the 2DM to the 1DM, while under the concentration difference or the hydraulic flow, the preferred direction for diffusion or streaming ionic current goes in the reversed direction, from the 1DM to the 2DM. A theoretical model based on coupled Poisson–Nernst–Planck and Navier–Stokes equations is employed to explain the asymmetric ion transport phenomena.

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Transport of ions and biomolecules through single asymmetric nanopores in polymer films

Cited by 94 publications

References 16 publications

PMMA Polymer Membrane-Based Single Cylindrical Submicron Pores: Electrical Characterization and Investigation of Their Applicability in Resistive-Pulse Biomolecule Detection

PMMA Polymer Membrane-Based Single Cylindrical Submicron Pores: Electrical Characterization and Investigation of Their Applicability in Resistive-Pulse Biomolecule Detection

Cylindrical nanochannels in ion-track polycarbonate membranes studied by small-angle X-ray scattering

Electrokinetically Controlled Asymmetric Ion Transport through 1D/2D Nanofluidic Heterojunctions

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