Transport of High Concentration of Thiamin, Riboflavin and Pyridoxine across Intestinal Epithelial Cells Caco-2

Abstract

SummaryIn this study, we examined the intestinal uptake of thiamin (vitamin B 1 ), riboflavin (vitamin B 2 ) and pyridoxine (vitamin B 6 ) administered at high concentration using intestinal epithelial Caco-2 cells as an in vitro model of drugs and food absorption. The effect of vitamin concentration, culture age, transport direction and incubation temperature on vitamin transport was determined. The vitamin transport was expressed as an apparent permeability coefficient and changes in cumulative fraction transported across epithelial membrane in time. It was found that transepithelial transport of these vitamins is dependent on the experimental factors. At low concentrations an active transport mechanism was observed, whereas at high vitamin concentration a passive transport dominated. At high vitamin concentration the transepithelial flux of vitamins in both directions was similar, which proves the mechanism of passive transport.

Pharmacokinetics of high-dose oral thiamine hydrochloride in healthy subjects

“…This was also found in an in vitro study [19]. Thomson found a linear relationship between urinary excretion and oral dose of thiamine between 10 and 50 mg [20].…”

Uptake and transport of B 12 -conjugated nanoparticles in airway epithelium

“…However, whilst in intestinal Caco-2 cells caveolae played no role in the internalisation of soluble B 12 , the data suggests that, to a degree, there might be an element of cross-talk between the two pathways in airway Calu-3 cells. Furthermore, paracellular transport of soluble vitamin B 12 cannot be ruled out, as suggested for other vitamin B family sub-types [42], which may partly contribute to the observed high level of permeability across the epithelial cells. Similar levels of transepithelial transport have also been reported in intestinal Caco-2 monolayers in studies using comparative (higher than physiological) doses of vitamin B 12 [43,44].…”

The Affinity of Carboplatin to B-Vitamins and Nucleobases

“…However, it is widely accepted that eating meals rich in vitamins and minerals, such as carrot and beetroot juice, reduces the likelihood of cancer, prevents it from metastasis, and may even help with cancer therapy. The concentration of B vitamins at the cellular level depends on the dose concentration of these vitamins and the type of transport [37][38][39]. On the other hand, after cisplatin chemotherapy, the patient receives small daily doses of vitamin B6 (2.5 mg per day), because this compound increases the bioavailability of magnesium ions, which in turn causes a decrease in the toxic effect of cisplatin during anticancer therapy, in particular the reduction of the neuropathy effect [40,41].…”