Transport of Glycerol by
            <i>Pseudomonas aeruginosa</i>

Tsay, San-San; Brown, Karen K.; Gaudy, Elizabeth T.

doi:10.1128/jb.108.1.82-88.1971

Cited by 31 publications

(16 citation statements)

References 19 publications

(7 reference statements)

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“…Kinetic data of AIB and L-alanine uptake in the thermophilic bacterium are characterized by biphasic, double-reciprocal curves with sharp breaks at about 100 gM, indicating the existence of two distinct saturable components with about 10-fold differences in the apparent Km values, which mediate the transport of these substrates into the cell. Similar kinetics for amino acids (2,14,15,17) as well as for other substrates (26) have been described in other microorganisms. It is not known as yet whether the observed kinetics for L-alanine and AIB are due to the presence of two different systems in the thermophilic organism, as reported for histidine in Salmonella typhimurium (2) and for aspartate in E. coli (14), or to the binding of the substrate to an allosteric site and reducing thereby the activity of its own permease (12).…”

Section: Discussionsupporting

confidence: 72%

Properties of α-Aminoisobutyric Acid Transport in a Thermophilic Microorganism

Reizer

Grossowicz

1974

J Bacteriol

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Uptake of a-aminoisobutyric acid (AIB) by a leucine-tyrosine auxotroph of a thermophilic microorganism starved for leucine was studied. AIB was taken up by the cells against a substantial concentration gradient (300: 1) and was present there in a free and unchanged form. Various energy inhibitors and sulfhydryl reagents strongly inhibited the accumulation of AIB. AIB uptake obeyed saturation kinetics, and the Lineweaver-Burk plot is characterized by a biphasic curve. AIB most probably shares a common transport system(s) with alanine, serine, and glycine. A mutant defective in L-alanine uptake was isolated by using the suicide effect due to accumulation of the tritiated substrate. The mutant also exhibited impaired transport activity towards AIB, glycine, and L-serine, but not to phenylalanine or valine. The transport of AIB, glycine, L-alanine, and L-serine was induced by D-alanine (5 x 10-3 M) during growth in a succinateand ammonia-containing medium. De novo protein synthesis was required for the induction of AIB transport; the induction was inhibited when growth occurred in glucose-containing media. The apparent differential rate of synthesis of the AIB transport system was decreased considerably in glucose-grown cells as compared to succinate-grown cells. A common genetic basis of either the regulatory or structural nature for the transport of AIB, alanine, glycine, and serine in a thermophilic microorganism is suggested. 0.45-,sm membrane filter (47-mm diameter, Sartorius-Membranfilter GmbH, Gittingen, W. Germany), washed, transferred to fresh medium (prewarmed to 55 C), and diluted to 0.1 mg (dry weight)/ml. Small 414 on August 3, 2020 by guest http://jb.asm.org/ Downloaded from 100:329-336. 9. Geck, P., E. Heinz, and B. Pfeiffer. 1972. The degree and the efficiency of coupling between the influx of Na+ and a-aminoisobutyrate in Ehrlich cells. Biochim.

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Section: Discussionsupporting

confidence: 72%

Properties of α-Aminoisobutyric Acid Transport in a Thermophilic Microorganism

Reizer

Grossowicz

1974

J Bacteriol

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“…Yet the K, value of strain P1525 for acetate (1.2 ,uM) is high compared to that of strain P17 (0.17 ,uM) for this substrate (28). Glucose and glycerol uptake by P. aeruginosa are dependent on inducible transport systems with a Kt value of about 8 p,M (8,17,24). This value, which is 10 times above the K, value of strain P17 for glucose (28), may explain the low growth rates of strain P1525 with glucose and glycerol, each at a concentration of 25 p,g of C per liter (Fig.…”

Section: Discussionmentioning

confidence: 93%

Growth of Pseudomonas aeruginosa in tap water in relation to utilization of substrates at concentrations of a few micrograms per liter

Kooij¹,

Oranje²,

Hijnen³

1982

Appl Environ Microbiol

100

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Five Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particular strain (P1525) isolated from drinking water. This strain was tested for the utilization of 30 compounds supplied at a concentration of 25 microgram of C per liter. The growth rate (number of generations per hour) of strain P1525 in this tap water was approximately 0.005 h-1, and with 10 compounds it was larger than 0.03 h-1. An average yield of 6.2 x 10(9) colony-forming units per mg of C was obtained from the maximum colony counts (colony-forming units per milliliter). The average yield and maximum colony count of strain P1525 grown in tap water supplied with a mixture of 45 compounds, each at a concentration of 1 microgram of C per liter, enabled us to calculate that 28 compounds were utilized. Growth rates of two P. aeruginosa strains (including P1525) in various types of water at 15 degrees C were half of those of a fluorescent pseudomonad. The concentrations of assimilable organic carbon calculated from maximum colony counts and average yield values amounted to 0.1 to 0.7% of the total organic carbon concentrations in five types of tap water. The assimilable organic carbon percentages were about 10 times larger in river water and in water after ozonation.

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“…Where indicated, nutrient broth was supplemented with 0.5%/ (wt/vol) glucose (GNB). The composition of minimal medium has been described previously (29). Compounds used as sole sources of carbon and energy were added to a final concentration of 0.5% (wt/vol).…”

Section: Methodsmentioning

confidence: 99%

Relationship between catabolism of glycerol and metabolism of hexosephosphate derivatives by Pseudomonas aeruginosa

Heath¹,

Gaudy²

1978

J Bacteriol

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The relationship between catabolism of glycerol and metabolism of hexosephosphate derivatives in Pseudomonas aeruginosa was studied by comparing the growth on glycerol and enzymatic constitution of strain PAO with these characteristics of glucose-catabolic mutants and revertants. Growth of strain PAO on glycerol induced a catabolic oxidized nicotinamide adenine dinucleotide-linked glyceraldehyde-phosphate dehydrogenase and seven glucose-catabolic enzymes. The results indicated that these enzymes were induced by a six-carbon metabolite of glucose. All strains possessed a constitutive anabolic Embden-Meyerhof-Parnas pathway allowing limited conversion of glycerol-derived triosephosphate to hexosephosphate derivatives, which was consistent with induction of these enzymes by glycerol. Phosphogluconate dehydratase-deficient mutants grew on glycerol. However, mutants lacking both phosphogluconate dehydrogenase and phosphogluconate dehydratase were unable to grow on glycerol, although these strains possessed all of the enzymes needed for degradation of glycerol. These mutants apparently were inhibited by hexosephosphate derivatives, which originated from glycerol-derived triosephosphate and could not be dissimilated. This conclusion was supported by the fact that revertants regaining only a limited capacity to degrade 6-phosphogluconate were glycerol positive but remained glucose negative.

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Transport of Glycerol by Pseudomonas aeruginosa

Cited by 31 publications

References 19 publications

Properties of α-Aminoisobutyric Acid Transport in a Thermophilic Microorganism

Properties of α-Aminoisobutyric Acid Transport in a Thermophilic Microorganism

Growth of Pseudomonas aeruginosa in tap water in relation to utilization of substrates at concentrations of a few micrograms per liter

Relationship between catabolism of glycerol and metabolism of hexosephosphate derivatives by Pseudomonas aeruginosa

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