Transport of digoxin-loaded Polymeric Nanoparticles Across BeWo cells, an
            <i>In Vitro</i>
            Model of Human Placental Trophoblast

Albekairi, Norah A.; Al-Enazy, Sanaalarab; Ali, Shariq; Rytting, Erik

doi:10.4155/tde.15.79

Cited by 27 publications

(17 citation statements)

References 39 publications

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“…TEER values between 30 and 60 Ω⋅cm 2 were considered acceptable for performing transport studies. 12 Moreover, antibody staining for the tight junction protein ZO-1 was performed on day 6 PS to examine barrier integrity in the confluent cell monolayer. DAPI counterstaining was used to visualize the nuclei.…”

Section: Monolayer Confirmationmentioning

confidence: 99%

“…This study provided a novel approach to the treatment of fetal diseases such as cardiac arrhythmia. 12 Grafmueller et al 13 analyzed the bidirectional transfer of plain and carboxylatemodified polystyrene particles ranging in size between 50 and 300 nm using the ex vivo human placental perfusion model. This study indicated that the main translocation mechanism is likely to involve an active, energy-dependent transport pathway.…”

Section: Introductionmentioning

confidence: 99%

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Uptake and transport of pullulan acetate nanoparticles in the BeWo b30 placental barrier cell model

Tang¹,

Jiang²,

He³

et al. 2018

IJN

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IntroductionNanomedicine has shown a great potential in perinatal medicine because of its characteristics of sustained, controlled release and targeting ability; on the other hand, it may also lead to unexpected toxicities such as embryotoxicity and even malformation after crossing the placental barrier, but data concerning transplacental transport are scarce. Pullulan acetate (PA) nanoparticles (NPs) are a promising nanocarrier derived from natural polysaccharide; however, their transplacental transport ability and mechanism are unknown.Materials and methodsIn this study, fluorescein isothiocyanate (FITC) conjugated PA (PA-FITC) was synthesized. PA-FITC NPs were characterized by dynamic light scattering, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The cytotoxicity of PA-FITC NPs at concentrations of 15, 30, 60, 125, 250, 500, 1,000 and 2,000 μg/mL was studied by cell counting kit-8. The human chorionic gonadotrophin (HCG) cytokine assay was conducted to evaluate the biological function of BeWo b30 cells. Endocytic mechanisms of PA-FITC NPs were investigated via fluorescence analysis. The monolayer properties were characterized by TEM, tight junction staining, transepithelial electrical resistance and fluorescein sodium transportation. The transport ability was measured in the cell based transwell model by confocal imaging and SEM.ResultsPA-FITC NPs were almost spherical shape with a size range of 200–300 nm. Cell viability of BeWo b30 cells was up to 100% in all groups. The concentrations of HCG increased with increasing numbers of cells and culture time, which showed the good biological function of BeWo b30 cells. PA-FITC NPs were rapidly endocytosed through caveolae-mediated endocytosis and pinocytosis, with uptake inhibition rates with nystatin (NY) and colchicines (Col) of 55% and 51% respectively. BeWo b30 cell monolayer was formed over 5 days. PA-FITC NPs were found in the cytoplasm of cells on the transwell membranes; while some NPs were found in the basolateral (fetal) compartment over 24 h.ConclusionIn summary, PA-FITC NPs are nontoxic, can cross the blood-placental barrier, and show mainly internalization to BeWo b30 cells through caveolae-mediated endocytosis and pinocytosis pathways, major via the former pathway. The results could benefit the adjustment and control of the transplacental transport of nanomedicines.

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Section: Monolayer Confirmationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Uptake and transport of pullulan acetate nanoparticles in the BeWo b30 placental barrier cell model

Tang¹,

Jiang²,

He³

et al. 2018

IJN

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“…It is already known that certain types of nanoparticles are capable of crossing the placental barrier, or causing indirect effects to placental or fetal development [53,100–102]. Endocytosis of nanoparticles by the placenta is important in that it can alter the transplacental passage of drugs.…”

Section: Mechanisms Of Drug Transport Across the Placentamentioning

confidence: 99%

“…Endocytosis of nanoparticles by the placenta is important in that it can alter the transplacental passage of drugs. An example of this is the passage of nanoparticles made of the polymer poly(lactic- co -glycolic acid) (PLGA) or its PEGylated block copolymer, which may alter or prevent drug binding to efflux transporters and potentially alter their metabolism [102,103]. Other nanoparticles, such as poly(amidoamine) dendrimers and silica nanoparticles, however, may demonstrate reduced transfer across the placenta as compared to polystyrene or PLGA nanoparticles [100,101,103,104].…”

Section: Mechanisms Of Drug Transport Across the Placentamentioning

confidence: 99%

Placental control of drug delivery

Al-Enazy

Ali

Albekairi

et al. 2017

Advanced Drug Delivery Reviews

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The placenta serves as the interface between the maternal and fetal circulations and regulates the transfer of oxygen, nutrients, and waste products. When exogenous substances are present in the maternal bloodstream—whether from environmental contact, occupational exposure, medication, or drug abuse—the extent to which this exposure affects the fetus is determined by transport and biotransformation processes in the placental barrier. Advances in drug delivery strategies are expected to improve the treatment of maternal and fetal diseases encountered during pregnancy.

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“…The BeWo cell line has been shown to be a useful in vitro placental barrier model for studying adhesion, transport, and infection [11], with many researchers using BeWo cells as an in vitro barrier model of the placenta to explore the transport and toxicity of foreign agents or organisms [12,13].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Profiling of Human Placental Trophoblasts in Response to Infection with Enterococcus faecalis

Tan

et al. 2018

Journal of Food Quality

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Increasing evidence suggests that Enterococcus faecalis strains can pass through placental barriers and cause adverse outcomes during pregnancy. However, the underlying molecular mechanism of the interaction between E. faecalis and the host placental barrier has yet to be fully elucidated. In this study, we have used DNA microarray analysis to investigate the response of human placental trophoblast-like BeWo cells to infection with E. faecalis OG1RF. These results indicate that a total of 2191 genes in BeWo cells are differentially expressed in the presence of E. faecalis OG1RF, with 1357 genes being upregulated and 834 genes being downregulated. These differentially expressed genes (DEGs) are involved in apoptosis, stress and stimulus response, placental and embryonic development, immune response, and cell adhesion. Therefore, these results provide information on the molecular mechanisms that E. faecalis invasion can trigger to cause adverse pregnancy outcomes.

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Transport of digoxin-loaded Polymeric Nanoparticles Across BeWo cells, an In Vitro Model of Human Placental Trophoblast

Cited by 27 publications

References 39 publications

Uptake and transport of pullulan acetate nanoparticles in the BeWo b30 placental barrier cell model

Uptake and transport of pullulan acetate nanoparticles in the BeWo b30 placental barrier cell model

Placental control of drug delivery

Transcriptomic Profiling of Human Placental Trophoblasts in Response to Infection with Enterococcus faecalis

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