Transport of cadmium and other metals in the blood of the bivalve mollusc Mercenaria mercenaria

Robinson, William E.; Ryan, David K.

doi:10.1007/bf00391250

Cited by 51 publications

(46 citation statements)

References 25 publications

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“…In addition, the ultrafiltrable fraction of the blood may contain metals complexed with citrate, bicarbonate, and phosphate as well as metals bound nonspecifically to carboxyl and amino residues of a variety of blood proteins. Despite this abundance of ligands, blood is not a major compartment for metal storage under normal conditions (Robinson and Ryan, 1988). However, after metal exposure (i.e., 100 ppb Cd) more metal is present in blood after 1 hour than in the soft tissues after 24 hours, as observed in the clam Mercenaria mercenaria (Robinson and Ryan, 1988).…”

Section: Metal Transport Between Tissuesmentioning

confidence: 97%

“…In addition, blood plasma proteins bind metals in a nonspecific way (Robinson and Ryan, 1988). In marine mussels and clams, metals such as Zn and Cd are transported primarily in blood plasma, associated with HMWP Pirie, 1980, Robinson andRyan, 1988). In the freshwater bivalve Anodonta cygnea, Cd is bound to either MT-like proteins or to HMWP (Helmeraad et al, 1986).…”

Section: Metal Transport Between Tissuesmentioning

confidence: 98%

“…Despite this abundance of ligands, blood is not a major compartment for metal storage under normal conditions (Robinson and Ryan, 1988). However, after metal exposure (i.e., 100 ppb Cd) more metal is present in blood after 1 hour than in the soft tissues after 24 hours, as observed in the clam Mercenaria mercenaria (Robinson and Ryan, 1988). Although George et al (1976) obtained X-ray pulses indicating the occurrence of Fe in hemocytes, Janssen and Ertelt-Janssen (1983) concluded that a considerable part of the Fe is passed to the blood plasma.…”

Section: Metal Transport Between Tissuesmentioning

confidence: 99%

“…Hemocytes (blood cells) might transport metals either associated with cytosolic proteins or within lysosomes. In addition, blood plasma proteins bind metals in a nonspecific way (Robinson and Ryan, 1988). In marine mussels and clams, metals such as Zn and Cd are transported primarily in blood plasma, associated with HMWP Pirie, 1980, Robinson andRyan, 1988).…”

Section: Metal Transport Between Tissuesmentioning

confidence: 99%

See 3 more Smart Citations

Cellular and subcellular distribution of metals in molluscs

Marigómez

Soto

Cajaraville

et al. 2002

Microscopy Res & Technique

414

202

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The cellular processes involved in metal metabolism in molluscs are reviewed, with emphasis on the contribution of microscopy (AMG, ARG, EPMA, and SIMS) to both basic research of metal cell biology and applied environmental research. In molluscs, metal uptake may occur by facilitated diffusion, active transport, or endocytosis, and can be enhanced by MT synthesis or formation of mineralized granules. In aquatic molluscs, gills constitute a key interface for dissolved metal uptake, where metals are bound to MT, incorporated into lysosomes, and released basally towards the blood plasma and circulating hemocytes. However, particulate metal uptake is mainly achieved via the digestive tract by endocytosis; further metals are transferred first to lysosomes and then to residual bodies, especially in the digestive cells of the digestive gland. Additionally, metals can be accumulated selectively in specific cell types. As ligands pools differ from cell to cell, different metals may be retained in different cell types. Class "a" metals are localized in cells with granules composed of carbonate, oxalate, phosphate, and sulfate (oxygen donors), whereas "b" metals are associated with those cell types rich in sulfur and nitrogen ligands (sulfur donors). In molluscs, oxygen donors occur in connective tissue calcium cells and basophilic cells, whereas sulfur donors are present in digestive cells, podocytes, nephrocytes, and rhogocytes. Hemocytes, which constitute the most relevant system for metal transport between tissues, move around the body and may penetrate tissues and remove metals from the inner medium to be accumulated in lysosomes as nondigested products. Rhogocytes also participate in metal mobilization, accumulation, and release. The assessment of metal levels in target cells of sentinel molluscs by microscopic techniques provides an early-warning measure, with promising applications as an exposure biomarker for environmental monitoring programs.

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Section: Metal Transport Between Tissuesmentioning

confidence: 97%

Section: Metal Transport Between Tissuesmentioning

confidence: 98%

Section: Metal Transport Between Tissuesmentioning

confidence: 99%

Section: Metal Transport Between Tissuesmentioning

confidence: 99%

See 2 more Smart Citations

Cellular and subcellular distribution of metals in molluscs

Marigómez

Soto

Cajaraville

et al. 2002

Microscopy Res & Technique

414

202

View full text Add to dashboard Cite

show abstract

“…Ce qui suggère que le plasma est le principal vecteur du métal (Robinson et Ryan, 1988) tout comme dans l'expérience de Roch et Maly ,1979 . Finalement ,pour évaluer la toxicité, le critère le plus utilisé se réfère au mode d'action du polluant. Cependant il n'est pas toujours facile de faire l'adéquation entre le taux de survie de l'animal et l'altération des processus biochimiques et physiologiques .…”

Section: -3 La Métallothionéine : Caractéristiques Et Rôlesunclassified

Effets de l intoxication au chlorure de cadmium chez la truite mouchetee Salvelinus fontinalis Mitchill

Brillant¹

1993

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Cadmium binding in the blood plasma of two marine bivalves

Robinson

Ryan

Sullivan

et al. 1997

Enviro Toxic and Chemistry

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Cadmium tansport in the plasma of Mytilus edulis L. displays attributes similar to those previously described for Mercenaria mercenaria (L.). The majority of Cr, Cu, Ni, and Zn is partitioned in the plasma rather than the hemocytes in both species, although differences in Fe and Mn are apparent. Mussels however contain twice the number of circulating hemocytes (2.1 ± 0.8% of whole blood weight; n = 75 vs 1.2 ± 0.3%; n = 70 for the quahog) and approximately three times more plasma protein than the quahog. Titration experiments using ion‐specific electrode (ISE) measurements and equilibrium dialysis (ED) experiments indicate that both species have a low‐affinity, high‐capacity system for the internal transport of Cd. At low blood Cd concentrations (<8.9 μM) and short dialysis times (24 h), approximately 90% of the Cd in mussel plasma is bound to plasma proteins. At higher Cd concentrations and at longer dialysis times, the percentage of free Cd increases substantially. The Cd affinity was slightly lower in mussel plasma compared to the quahog (log10K = 2.6–3.9/M vs 3.7–4.3/M), and mussel plasma CL values were appreciably lower (0.16–1.11 mmol/g protein) than those for the quahog (1.97–2.26 mmol/g protein). Both ISE and ED experiments on quahog plasma yielded similar estimates of K and CL.

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Transport of cadmium and other metals in the blood of the bivalve mollusc Mercenaria mercenaria

Cited by 51 publications

References 25 publications

Cellular and subcellular distribution of metals in molluscs

Cellular and subcellular distribution of metals in molluscs

Effets de l intoxication au chlorure de cadmium chez la truite mouchetee Salvelinus fontinalis Mitchill

Cadmium binding in the blood plasma of two marine bivalves

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