Transport of butyrate across the isolated bovine rumen epithelium — interaction with sodium, chloride and bicarbonate

Sehested, Jakob; Diernæs, L; Møller, Per; Skadhauge, Erik

doi:10.1016/s1095-6433(99)00082-3

Cited by 40 publications

(50 citation statements)

References 18 publications

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“…A recent report indicates similar ontogenic and physiological effects on sodium and chloride transport in the developing calf rumen epithelium (Breves et al, 2002). Increased sodium and chloride transport by the developing rumen epithelium could reflect an increase in the SCFA absorptive capacity by the rumen epithelium (Sehested et al, 1999). Shen et al (2004) observed higher IGF-1 concentration in plasma, increased papillae size and surface of rumen epithelium, and an enhanced net flux of Na + across the isolated rumen epithelium in kids fed high energy levels.…”

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confidence: 82%

Diet induced ruminal papillae development in neonatal calves not correlating with rumen butyrate

Žitňan¹,

Kuhla²,

Sanftleben³

et al. 2005

Vet. Med. - Czech

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ABSTRACT:The objective of this study was to investigate the development of rumen mucosa and the level of plasma IGF-1 in calves induced by different amounts and types of milk replacers and solid diet. Forty-five male Holstein calves 7 days of age were assigned to three groups: group I milk free replacer, late weaned; group II milk free replacer, early weaned, and group III milk replacer, early weaned. All animals received additional concentrate, water and maize silage were offered ad libitum. In each group, three calves were slaughtered at 41 days of age. The concentration of ruminal total SCFA and the molar proportion of butyrate did not differ between the groups, but the molar proportion of acetate was lower (P = 0.01) and the proportion of propionate was higher (P = 0.02) in early weaned calves. Compared to the late weaned calves (group I) the length, width and surface of the papillae of atrium ruminis, the length and width of the papillae of ventral ruminal sac and the length of the papillae of ventral blind sac were greater (P < 0.05) in the early weaned calves fed low amounts of milk and high amounts of concentrate (group III). Furthermore, there was a tendency of plasma IGF-1 concentration to be increased (P = 0.1) in early weaned calves. The plasma levels of glucose and insulin were decreased (P < 0.01, and P = 0.03, respectively). Positive correlations existed between papillae length and plasma IGF-1 concentrations (P < 0.10). Insulin and glucose concentrations were negatively correlated with parameters of papillae development (P < 0.1). In conclusion, the development of rumen papillae was stimulated in calves consuming increased amounts of concentrate. The effect was not correlated with the molar proportion of butyrate, but with the molar propionate proportion in the rumen and with the plasma IGF-1 concentration.

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confidence: 82%

Diet induced ruminal papillae development in neonatal calves not correlating with rumen butyrate

Žitňan¹,

Kuhla²,

Sanftleben³

et al. 2005

Vet. Med. - Czech

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“…Interestingly, this conductance could also be blocked by pCMB and DIDS, which implies that some of the SCFA transport rates previously attributed to anion exchangers or monocarboxylate transporters because of blocker sensitivity (Sehested et al, 1999;Kirat et al, 2006) might additionally or alternatively be explained by a contribution of anion channels. The permeability of the channel decreases with increasing chain length, which might explain why butyrate is retained and metabolized by the ruminal epithelium (F. Stumpff, unpublished data).…”

mentioning

confidence: 89%

“…The majority of these SCFA (which consist mainly of acetate (,60%), propionate (,20%) and butyrate (,10%) are absorbed across the forestomachs and cover a substantial part of the animals energy requirements (Bergman, 1990). The major fraction of SCFA crosses the forestomach epithelia unmetabolized (Kiddle et al, 1951;Gä bel et al, 2002), although SCFA, in particular butyrate, are also used by the epithelial cells themselves for cell metabolism, which could support active transport of other electrolytes (Weigand et al, 1975;Sehested et al, 1999;Kristensen et al, 2000;Gä bel et al, 2002;Gä bel and Aschenbach, 2006). Apical uptake mechanisms for SCFA Previous studies and reviews have focussed on two possible mechanisms of SCFA uptake across the apical membrane: diffusion of the protonated, undissociated form through the lipid bilayer and exchange of the dissociated SCFA 2 anion for bicarbonate via a transport protein that exchanges SCFA 2 for HCO 3 2 anions under physiological conditions (Figure 4, Gä bel et al, 1991a;Kramer et al, 1996;Gä bel et al, 2002;Aschenbach et al, 2009).…”

Section: Absorption Of Scfasmentioning

confidence: 99%

“…Previous attempts to observe a corresponding drop in SCFA fluxes across ruminal epithelia were unsuccessful, which may be related to the higher pH of 7.4 at which these studies were performed (Kramer et al, 1996;Sehested et al, 1999), but may also reflect more profound differences between the two tissues. A major difference between the omasum and the rumen is the direction of transport of the apical Cl 2 /HCO 3 2 exchanger, mediated by the presence of an apical NaCl cotransporter in the omasum that is absent in the rumen.…”

mentioning

confidence: 99%

“…Without SCFA gradients, however, a small net absorption of butyrate across bovine rumen could also be measured, but only in the presence of bicarbonate, and tended to be reduced by the presence of Cl 2 (Sehested et al, 1999). In the reticulorumen of sheep in vivo, that is in the presence of SCFA gradients, the disappearance of butyrate was not affected by Cl 2 , while butyrate slightly reduced the disappearance of chloride (Aschenbach et al, 2009), pointing to a small additional contribution of anion exchangers to butyrate absorption and a possible competition between butyrate and Cl 2 absorption.…”

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confidence: 99%

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Transport of cations and anions across forestomach epithelia: conclusions from in vitro studies

Leonhard-Marek

Stumpff

Martens

2010

Animal

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Secretion of saliva as well as absorptive and secretory processes across forestomach epithelia ensures an optimal environment for microbial digestion in the forestomachs. Daily salivary secretion of sodium (Na 1 ) exceeds the amount found in plasma by a factor of 2 to 3, while the secretion of bicarbonate (HCO 3 2 ) is 6 to 8 times higher than the amount of HCO 3 2 in the total extracellular space. This implies a need for efficient absorptive mechanisms across forestomach epithelia to allow for an early recycling. While Na 1 is absorbed from all forestomachs via Na 1 /H 1 exchange and a non-selective cation channel that shows increased conductance at low concentrations of Mg 21 , Ca 21 or H 1 in the luminal microclima and at low intracellular Mg 21 , HCO 3 2 is secreted by the rumen for the buffering of ingesta but absorbed by the omasum to prevent liberation of CO 2 in the abomasum. Fermentation provides short chain fatty acids and ammonia (NH 3 ) that have to be absorbed both to meet nutrient requirements and maintain ruminal homeostasis of pH and osmolarity. The rumen is an important location for the absorption of essential minerals such as Mg 21 from the diet. Other ions can be absorbed, if delivered in sufficient amounts (Ca 21 , P i , K 1 , Cl 2 and NH 4 1 ). Although the presence of transport mechanisms for these electrolytes has been described earlier, our knowledge about their nature, regulation and crosstalk has increased greatly in the last years. New transport pathways have recently been added to our picture of epithelial transport across rumen and omasum, including an apical non-selective cation conductance, a basolateral anion conductance, an apical H 1 -ATPase, differently expressed anion exchangers and monocarboxylate transporters.Keywords: ruminants, sodium potassium magnesium and calcium, short chain fatty acids, chloride and bicarbonate, channels transporters and exchangers ImplicationsThe improved knowledge of ruminal ion transport clearly underlines its physiological meaning for the whole animal. For example, magnesium absorption is markedly reduced at low Mg and high K intake and the effect of potassium is diminished at high Mg intake. This variable interaction has been quantified recently permitting the prediction of Mg absorption. Great progress has also been made in understanding the interactions between the absorptive pathways for Na, short chain fatty acids and ammonium. The new findings on structure and regulation of various ion transporters will allow a better understanding of the challenges that different diets pose to the maintenance of homeostatic conditions within the rumen and within the cells of the surrounding epithelium, with the implications for the investigation of ruminal adaptation to different diets. Future studies on transport pathways should include the barrier function of rumen epithelium and its possible impairment under harsh feeding conditions.

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Effects of seasonal changes in food quality and food intake on the transport of sodium and butyrate across ruminal epithelium of reindeer

Storeheier

Sehested²,

Diernæs³

et al. 2003

Journal of Comparative Physiology B: Biochemical, Systemic, and

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Transport of 22Na and 14C-butyrate across the ruminal epithelium of captive reindeer fed a concentrate diet in summer (n=5) and in winter (n=5) and from free-ranging reindeer taken from summer (n=3) and winter pasture (n=5) was measured in vitro in Ussing chambers. Significant amounts of both Na+ and butyrate were transported across the isolated epithelium without any external driving force. The ruminal transport of Na+ and butyrate were interacting, as evidenced by both the observed amiloride-induced reduction of net butyrate-transport and by the positive correlation between net transport of butyrate and Na+. Amiloride also reduced the net transport of Na+ without significantly affecting the short-circuit current, indicating the presence of an apical Na+/H+ exchanger in the ruminal epithelium of reindeer. The captive reindeer increased the dry matter intake of a constant quality concentrate from winter to summer, but this neither affected their ruminal transport capacity nor their ruminal surface enlargement factor (SEF). Free-ranging reindeer increased their ruminal transport capacity for Na+ and butyrate from summer to winter but simultaneously reduced their ruminal SEF. The present data indicate that this food-induced increase in transport capacity was attributed to changes in the nutrient composition of the diet.

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Transport of butyrate across the isolated bovine rumen epithelium — interaction with sodium, chloride and bicarbonate

Cited by 40 publications

References 18 publications

Diet induced ruminal papillae development in neonatal calves not correlating with rumen butyrate

Diet induced ruminal papillae development in neonatal calves not correlating with rumen butyrate

Transport of cations and anions across forestomach epithelia: conclusions from in vitro studies

Effects of seasonal changes in food quality and food intake on the transport of sodium and butyrate across ruminal epithelium of reindeer

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