Transport, metabolism and remedial potential of functional food extracts (FFEs) in Caco-2 cells monolayer: A review

Iftikhar, Maryam; Iftikhar, Asra; Zhang, Huijuan; Gong, Lingxiao

doi:10.1016/j.foodres.2020.109240

Cited by 46 publications

(39 citation statements)

References 136 publications

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“…It was originally derived from a heterogeneous human epithelial colorectal adenocarcinoma cells established by Fogh and coworkers in 1977 [ 189 ]. The Caco-2 cells have the ability to spontaneously differentiate into a monolayer of cells, expressing many properties typical of absorptive enterocytes with a brush border layer, tight junctions, and efflux and uptake transporters as found in the small intestine [ 190 , 191 , 192 ]. Moreover, several phenolic compounds (e.g., kaempferol) are able to regulate the MAPK pathway, which is beneficial to the barrier functions [ 193 ].…”

Section: Assessment Of Bioavailability Of Mycotoxins Using Caco-2 mentioning

confidence: 99%

Mycotoxins: Biotransformation and Bioavailability Assessment Using Caco-2 Cell Monolayer

Tran

Viktorová

Ruml

2020

Toxins

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The determination of mycotoxins content in food is not sufficient for the prediction of their potential in vivo cytotoxicity because it does not reflect their bioavailability and mutual interactions within complex matrices, which may significantly alter the toxic effects. Moreover, many mycotoxins undergo biotransformation and metabolization during the intestinal absorption process. Biotransformation is predominantly the conversion of mycotoxins meditated by cytochrome P450 and other enzymes. This should transform the toxins to nontoxic metabolites but it may possibly result in unexpectedly high toxicity. Therefore, the verification of biotransformation and bioavailability provides valuable information to correctly interpret occurrence data and biomonitoring results. Among all of the methods available, the in vitro models using monolayer formed by epithelial cells from the human colon (Caco-2 cell) have been extensively used for evaluating the permeability, bioavailability, intestinal transport, and metabolism of toxic and biologically active compounds. Here, the strengths and limitations of both in vivo and in vitro techniques used to determine bioavailability are reviewed, along with current detailed data about biotransformation of mycotoxins. Furthermore, the molecular mechanism of mycotoxin effects is also discussed regarding the disorder of intestinal barrier integrity induced by mycotoxins.

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Section: Assessment Of Bioavailability Of Mycotoxins Using Caco-2 mentioning

confidence: 99%

Mycotoxins: Biotransformation and Bioavailability Assessment Using Caco-2 Cell Monolayer

Tran

Viktorová

Ruml

2020

Toxins

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“…Geralmente as células Caco-2 desempenham quatro tipos de transporte celular de nutrientes para serem absorvidos, são eles: difusão passiva, transporte paracelular, transporte transcitose e endocitose (IFTIKHAR et al, 2020).…”

Section: 5 25 Biodisponibilidade Bioacessibilidade E Bioatividadeunclassified

“…Essa membrana porosa funciona como mediadora do efluxo de compostos da fase apical (fase bioacessível) para a fase basolateral (compostos absorvidos pela parede celular) (HUBATSCH; RAGNARSSON; ARTURSSON, 2007). Este ensaio é considerado adequado para a avaliação da manutenção da capacidade antioxidante de alimentos funcionais e produtos naturais após o processo de digestão e transporte celular (IFTIKHAR et al, 2020). Entretanto, existem algumas limitações no uso de células Caco-2 para estudos de absorção, como por exemplo, o fato das células serem estáticas quanto comparadas à parede intestinal, que está sempre realizando o movimento de peristaltismo; o espaço intercelular apresentado pelas juntas oclusivas é relativamente menor (5Å) que o real espaço tido entre os enterócitos (8-13Å); demasiado tempo para estabilizar a diferenciação da célula em modelos de enterócitos (21 dias) e o tempo de integridade relativamente curto das células (7 dias), considerando que seu fenótipo é alterado a cada dia (TAVELIN, 2003).…”

Section: 5 25 Biodisponibilidade Bioacessibilidade E Bioatividadeunclassified

“…Sendo assim, apesar de algumas limitações, o modelo de transporte epitelial ex vivo utilizando células Caco-2 se demonstra conveniente para estudos das propriedades funcionais apresentadas por extratos de alimentos e produtos naturais (IFTIKHAR et al, 2020).…”

Section: 5 25 Biodisponibilidade Bioacessibilidade E Bioatividadeunclassified

“…Ou seja, há uma mudança na conformação química dos compostos, e o modelo de digestão in vitro utilizado neste estudo não contempla a etapa de biotransformação e atuação dos microrganismos sob os compostos da matriz alimentar presentes na fase intestinal.O modelo de monocamada de células Caco-2 possuí algumas limitações quando comparado aos enterócitos que constituem o epitélio intestinal. Entre as limitações está a baixa permeabilidade paracelular (IFTIKHAR et al, 2020), dificultando a absorção de compostos que possuem esse tipo de transporte, como é o caso do ácido cafeico. No entanto, foi possível verificar que mesmo após o processo de digestão e transporte, alguns compostos presentes no extrato etanólico da PO1, e com atividade antioxidante, foram absorvidos.…”

Section: 33 Digestão Gastrointestinal In Vitrounclassified

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Food‐borne polyphenols: A biocompatible anchor recuperating iron homeostasis

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Food‐borne polyphenols have long contributed to human health via multifaceted mechanisms, while emerged studies especially those published in recent 10 years have witnessed the close relevance between the bioactivity of polyphenols with iron homeostasis. Polyphenols are involved in various physiological processes of iron and manifest with multidirectional regulative effects. The diversiform polyphenols–iron interactions have extended the cognition of various disorders and diseases. This review aims to present a comprehensive scope of polyphenols–iron interactions, and the structural property, biological effects, and molecular mechanism of polyphenols on iron homeostasis were systematically illustrated, categorized, exemplified, and discussed. Multiple related disorders and diseases were focused on, and the specific polyphenols–iron interactions were elucidated during the processes of pathogenesis, development, intervention, and treatment. Present shortages such as bidirectional effects, unclear mechanism, clinical challenges, and extraction efficiency of polyphenols were also analyzed, and some innovative techniques adopted for polyphenols–iron‐based systems or therapies explorations were discussed, inspiring future perspectives for the understandings, exploitations, and correlations of both iron homeostasis and polyphenols. This review brings the polyphenols–iron interactions on the stage, telling the full story of how polyphenols anchor iron to achieve beneficial bioactivities, enlightening ideas for polyphenols–iron interactions‐based understanding of diseases, and illumining strategies for polyphenols as dietary adjuvant or future therapeutic agents for iron‐related physiological abnormality.

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Transport, metabolism and remedial potential of functional food extracts (FFEs) in Caco-2 cells monolayer: A review

Cited by 46 publications

References 136 publications

Mycotoxins: Biotransformation and Bioavailability Assessment Using Caco-2 Cell Monolayer

Mycotoxins: Biotransformation and Bioavailability Assessment Using Caco-2 Cell Monolayer

Digestão >i<in vitro>/i< e transporte de polifenóis de própolis orgânica do Sul do Brasil por mmeio de células humanas Caco-2

Food‐borne polyphenols: A biocompatible anchor recuperating iron homeostasis

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