Transport in Cells of Storage Tissues

Poole, Ronald J.

doi:10.1007/978-3-642-66227-0_8

Cited by 20 publications

(11 citation statements)

References 70 publications

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“…Disks about 0.8 mm thick and 4 mm diameter were cut from storage tissue of red beet (Beta vulgaris L.), and washed in 0.1 mm aerated CaSO4, changed daily, at 22-24 C. The washing was continued for [5][6][7] days, except where otherwise indicated, in order to allow development of Cl-transport (12 15,000g. Eight-ml samples of supernatant + 10 ml Aquasol (New England Nuclear) were counted in a scintillation counter, and then recounted for 36C1 after decay of the 42K.…”

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confidence: 99%

ATP Levels and their Effects on Plasmalemma Influxes of Potassium Chloride in Red Beet

Petraglia¹,

Poole²

1980

Plant Physiol.

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Tissue ATP concentrations in slices of red beet increase progressively with time for up to 7 days after cutting the root. ATP The manner in which metabolic energy is supplied to ion transport processes in plant cells is not well established. It is generally agreed that cation influx can be coupled to ATP hydrolysis (13), although the manner of coupling, and the relationship of cation influx to electrogenic H+ efflux, remains in doubt. In the present study, the dependence of K+ influx on ATP concentration has been investigated in more detail. We show in another report (14) that the present relationship between influx and ATP concentration is different from that observed for the electrogenic pump activity.With regard to anion influx (13), there is some evidence for its dependence on ATP but contrary claims have also been made, especially for storage tissues (2, 11). The present study shows that in storage tissue of red beet Cl-influx shows a dependence on ATP concentration similar to that observed for K+ influx. Evidence is also presented that, in addition to its effect on ATP levels, oligomycin inhibits the Cl-pump at the plasmalemma. (1) with modifications as indicated below. One g beet disks frozen in liquid N2 were disrupted in 40 ml ice-cold 0.4 N HC104 + 1 mm EDTA disodium salt, in a Virtis homogenizer for 1 min. One-half of the homogenate was taken for isotope counting. This was decolorized with activated charcoal (2.5 ml of a 10%7b suspension in water), and centrifuged 10 min at 15,000g. Eight-ml samples of supernatant + 10 ml Aquasol (New England Nuclear) were counted in a scintillation counter, and then recounted for 36C1 after decay of the 42K. MATERIALS AND METHODSThe remainder of the tissue homogenate was taken for ATP assays after centrifugation at 10,000g for 15 min to sediment debris. Immediately before the ATP assays, 5 ml ofthe supernatant were added to 5 ml 40 mm glycylglycine buffer (pH 7.4) and the mixture was adjusted to pH 7.4 with 1 M KOH + 0.1 M KHCO3. The extract was then centrifuged at 5,000g for 5 min to remove KC104 and ATP assays were performed without delay. The luciferin-luciferase ATP assays were performed without delay. The luciferin-luciferase ATP assays were performed as described by Schram (15) with a few modifications. The content of one vial of firefly extract (Sigma FLE-50) was dissolved in 5 ml cold distilled H20. This reconstitution of the firefly extract gives a final concentration of 50 mm potassium arsenate and 20 mm MgSO4 (pH 7.4). This enzyme solution was agitated occasionally and incubated on ice for 2-5 h. When ready for use, the enzyme solution was centrifuged 10 min at 5,000g. The supernatant was either used immediately in the ATP assay or stored frozen for future use.To scintillation vials containing 700 Id glycylglycine buffer were added 100 tA of the enzyme solution prepared as described above.

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confidence: 99%

ATP Levels and their Effects on Plasmalemma Influxes of Potassium Chloride in Red Beet

Petraglia¹,

Poole²

1980

Plant Physiol.

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“…Although transport properties of vascular and parenchyma cells have been reported to be similar (Poole, 1976), we suspected that the differences in development of cation transport capacity in vascular and parenchyma cells are the source of the variability observed. Our findings are described in this report.…”

Section: Introductionmentioning

confidence: 89%

“…Slices of storage tissue from red table beet (Beta vulgaris L.) subjected to a period of washing, usually referred to as ageing, are often used as model tissue for ion uptake studies (Sutcliffe, 1957;Briggs, Hope, and Pitman, 1958;Van Steveninck, 1975;Poole, 1976;Francois, Bogemans, and Neirinckx, 1982). A common feature of beet storage tissues is the presence of a series of concentric rings of small vascular cells (xylem and phloem) that are separated from one another by broader bands of highly vacuolated thin-walled parenchyma cells (Artschwager, 1926;Hayward, 1938).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Localized Intense Cation Uptake Sites in the Vascular Rings of Red Beet Storage Tissue 2

Mozafar¹,

Oertli²

1984

J Exp Bot

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Development and decline of cation uptake capacity in discs taken from the vascular and parenchyma rings of storage tissue of red table beet (Beta vulgaris L.) were observed during 12 d of ageing. Uptake capacity for Na + and Rb + showed a steady rise reaching maximums by the fourth to fifth days of ageing. Thereafter, there was a steady decline in the uptake rates. Vascular ring tissues were able to develop a greater uptake capacity for both Na + and Rb + than the tissues of parenchyma rings. This difference, which was more pronounced for Rb + than for Na + uptake, is attributed to a combination of variations in cell density and differences in the acquisition and retention of the cation uptake capacity. Respiration of tissue discs showed no significant rise during ageing, nor were there significant differences in the respiration of vascular and parenchyma tissues. Vascular tissues contained significantly more betacyanin than parenchyma tissues; and they retained their pigment, as well as their acquired cation uptake capacity, for a longer period during the ageing process.

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“…Ca2+ often increases influx rates in red beet, it does not seem to be required to maintain membrane integrity, perhaps because it is already present in the cell wall (4). In all cases, there was a 30-mi period of pretreatment in the unlabeled experimental solution in air, before any experimental treatments were applied.…”

Section: Methodsmentioning

confidence: 99%

Effect of Anoxia on ATP Levels and Ion Transport Rates in Red Beet

Petraglia¹,

Poole²

1980

Plant Physiol.

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in closed 25-ml flasks containing 5-7 ml experimental solution at 30 C. Peristaltic pumps were used to empty and refill the flasks at intervals without exposure to air. N2 treatments were applied by passing 02-free N2 gas into the flasks and at the same time replacing the solutions with solutions equilibrated with N2. For measurements of ATP content under N2, liquid N2 was poured into the flasks to freeze the tissue before exposing it to air. The frozen tissue was then extracted as described previously (2). RESULTS AND DISCUSSIONIn the preceding paper (2), we have presented evidence that the plasmalemma influxes of K+ and Cl-in storage tissue of red beet are both closely dependent on the ATP level in the tissue. This conclusion is contrary to that of Polya and Atkinson (3). Among the lines of evidence adduced by the latter investigators in favor of another energy source is that a N2 atmosphere did not appear to alter ATP levels, although it strongly inhibited ion fluxes. Here, we repeat the measurements ofATP content under N2 atmosphere, with opposite results to those of Polya and Atkinson (3). We also show that plasmalemma fluxes are inhibited within 1 min or less following removal of 02. This response is too rapid to be attributable to ATP-dependent changes in tonoplast transport, and thus indicates a more direct dependence of plasmalemma influxes on ATP.

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Transport in Cells of Storage Tissues

Cited by 20 publications

References 70 publications

ATP Levels and their Effects on Plasmalemma Influxes of Potassium Chloride in Red Beet

ATP Levels and their Effects on Plasmalemma Influxes of Potassium Chloride in Red Beet

Occurrence of Localized Intense Cation Uptake Sites in the Vascular Rings of Red Beet Storage Tissue 2

Effect of Anoxia on ATP Levels and Ion Transport Rates in Red Beet

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