Transport and Recovery of Turbot (Scophthalmus maximus) Sedated with MS-222 and Eugenol: Effects on Intermediary Metabolism and Osmoregulation

Abstract

This study focused on the anesthetic waterless keep-alive transport technique for turbot. MS-222 and eugenol were used to anesthetize turbot and then the waterless keep-alive transport was conducted. The blood physiological changes and flesh quality changes of turbot were evaluated after cooling and during the simulated waterless transport. The results show that the temperature lowered from 13 to 2 °C, resulting in a decrease in moisture, fat and protein contents of all samples. Compared to the control turbot, the turbots treated with MS-222 and eugenol presented higher pH and glycogen content. During the simulated waterless transport, the pH, ATP and glycogen contents in MS-222- and eugenol-treated turbots decreased and the IMP and lactate levels increased. For the blood biochemical indices, blood glucose, cortisol and urea nitrogen increased with the increase in transport time in MS-222- and eugenol-treated turbots. At sampling time, the changes in blood physiological indices were significantly higher in the control samples than those in the MS-222- and eugenol-treated samples. The results indicate that the turbot samples treated with MS-222 or eugenol could reduce stress during cooling and simulated waterless transport.

The protective role of iodine and MS-222 against stress response and bacterial infections during Nile tilapia (Oreochromis niloticus) transportation

“…Similarly, Cao et al (2021) found that MS-222 and eugenol-anesthetised turbot (Scophthalmus maximus) has lower blood glucose and cortisol level than the control (non-anesthetised) groups. Navarro et al (2016) also stated that plasma cortisol and glucose of the anaesthetized O. niloticus were lower than those of untreated controls.…”

Effects of Eugenol on Water Quality and the Metabolism and Antioxidant Capacity of Juvenile Greater Amberjack (Seriola dumerili) under Simulated Transport Conditions

“…The body maintains a relatively constant glucose concentration through the synthesis and catabolism of liver glycogen [ 46 ]. Previous studies have shown that aquatic animals respond to the stress of low temperatures and transport by breaking down liver glycogen to maintain the body’s energy requirements [ 47 , 48 ]. Eugenol has also been found by previous studies to relieve transportation stress, with significantly reduced serum levels of COR (cortisol) and GOT (glutamic oxaloacetic transaminase) found in hybrid amazon catfish ( Pseu-doplatystoma reticulatum × Leiarius marmoratus ) anesthetized by eugenol [ 49 ].…”

The Effects of Lemon Balm (Melissa officinalis L.) Essential Oil on the Stress Response, Anti-Oxidative Ability, and Kidney Metabolism of Sea Bass during Live Transport

“…Six groups were used in this study. The concentrations of MO in the transport water were 10 (10 MO), 20 (20 MO), and 40 (40 MO) mg/L; MS-222 was 30 mg/L (MS-222) [28]; and eugenol was 20 mg/L (Eugenol) [29], respectively. The character of MS-222 was weakly acidic, and the pH value range of the transport water after adding 30 mg/L MS-222 was 7.0~8.0.…”