Transport and programmed release of nanoscale cargo from cells by using NETosis

Abstract

Immune cells take up nanoscale materials and can be programmed to release it again, which has important implications for understanding cellular functions, biocompatibility as well as biomedical applications.

Quantum Defects as a Toolbox for the Covalent Functionalization of Carbon Nanotubes with Peptides and Proteins

“…[32] This interesting impact on the SWCNT photophysics could be attributed to the peptides folding differently on the SWCNT and changing the charge landscape through which the exciton diffuses or where it gets trapped, thus leading to enhanced E 11 * fluorescence for less hydrophobic sequences.D ifferent folding of peptides is known from noncovalent SWCNT/peptide hybrids. [61] Furthermore,acomparison of the sequences 9-12 consisting of identical amino acids shows that not only the nature of the attached amino acids,but also their sequential arrangement is of high importance for the SWCNT* NIR fluorescence properties.T hese results demonstrate the possibilities of employing Fmoc-protected phenylalanine defects for the growth of peptidic chains directly on the nanotubessidewall and indicate that this method can not only be used for modulation of the SWCNTs fluorescence,b ut also to tailor their surface properties.T his in turn could enable SWCNTs with enhanced cellular uptake/retention, [62,63] tailored molecular recognition motifs,a nd novel and more stable optical sensors operating in the NIR. Furthermore,t he coupling of asecond optically active molecule (fluorophore) via apeptide sequence to aSWCNT could serve as general design principle for molecular recognition and signal transduction.…”

“…These results demonstrate the possibilities of employing Fmoc‐protected phenylalanine defects for the growth of peptidic chains directly on the nanotube's sidewall and indicate that this method can not only be used for modulation of the SWCNTs’ fluorescence, but also to tailor their surface properties. This in turn could enable SWCNTs with enhanced cellular uptake/retention, tailored molecular recognition motifs, and novel and more stable optical sensors operating in the NIR. Furthermore, the coupling of a second optically active molecule (fluorophore) via a peptide sequence to a SWCNT could serve as general design principle for molecular recognition and signal transduction.…”

Exfoliation and Optical Properties of Near-Infrared Fluorescent Silicate Nanosheets

“…Therefore, single nanoparticle fluorescence applications such as microrheology or, in the future, chemical sensing [51,[57][58][59] should be possible.…”

Quantendefekte als Werkzeugkasten für die kovalente Funktionalisierung von Kohlenstoffnanoröhren mit Peptiden und Proteinen

“…Diese Ergebnisse demonstrieren die Mçglichkeiten, die der Einsatz von Fmoc-geschützten Phenylalanindefekten fürd as Wachstum von Peptidketten direkt auf der Seitenwand von SWCNTs ermçglicht und zeigen, dass diese Methode nicht nur fürdie Modulation der SWCNT-Fluoreszenz, sondern auch fürd as Maßschneidern ihrer Oberflächeneigenschafteng enutzt werden kann. Dies wiederum kçnnte es ermçglichen, SWCNTs mit verbesserter zellulärer Aufnahme/Verweildauer, [62,63] maßgeschneiderte molekulare Erkennungseinheiten oder neue und stabilere optische Sensoren, welche im NIR operieren, herzustellen. Zusätzlich kçnnte das Kuppeln eines zweiten optisch aktiven Moleküls (Fluorophor) durch eine Peptidsequenz an eine SWCNT als generelles Designprinzip zur molekularen Erkennung und Signaltransduktion dienen.…”