Transport and Polymerization of Porphyromonas gingivalis Type V Pili

Shoji, Mikio; Shibata, Satoshi; Naito, Mariko; Nakayama, Koji

doi:10.1007/978-1-0716-0939-2_7

Cited by 3 publications

(4 citation statements)

References 20 publications

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“…The cell pellets were lysed by PBS with the same volume of the supernatant fraction. The cell lysates and the supernatant fraction were mixed with Laemmli's SDS sample buffer containing appropriate amounts of TLCK and leupeptin (Shoji et al., 2021).…”

Section: Methodsmentioning

confidence: 99%

“…To determine the inhibitory effect of the C‐terminal peptide on FimA polymerization, an overnight preculture (0.5 mL) was inoculated in 5 mL BHI liquid medium and incubated anaerobically for 3 h. Then, 0.1 mM C‐terminal peptides (H364‐W383, H364‐W374, W374‐W383, and H364‐W383 scrambled peptide mix #1 and #2, respectively) were added in the presence of 100 ng/mL anhydrotetracycline (aTC) and incubated anaerobically for an additional 4 h. Cell lysates were prepared as previously described (Shoji et al., 2021).…”

Section: Methodsmentioning

confidence: 99%

“…and incubated anaerobically for an additional 4 h. Cell lysates were prepared as previously described (Shoji et al, 2021).…”

Section: Sample Preparation For Sds-pagementioning

confidence: 99%

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A conditional gene expression system in Porphyromonas gingivalis for study of the secretion mechanisms of lipoproteins and T9SS cargo proteins

Sasaki

Shoji

Sueyoshi

et al. 2023

Molecular Oral Microbiology

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The Gram‐negative anaerobe, Porphyromonas gingivalis, is known to be a pathogen associated with chronic periodontitis. P. gingivalis possesses virulence factors such as fimbriae and gingipain proteinases. Fimbrial proteins are secreted to the cell surface as lipoproteins. In contrast, gingipain proteinases are secreted into the bacterial cell surface via the type IX secretion system (T9SS). The transport mechanisms of lipoproteins and T9SS cargo proteins are entirely different and remain unknown. Therefore, using the Tet‐on system developed for the genus Bacteroides, we newly created a conditional gene expression system in P. gingivalis. We succeeded in establishing conditional expression of nanoluciferase and its derivatives for lipoprotein export, of FimA for a representative of lipoprotein export, and of T9SS cargo proteins such as Hbp35 and PorA for representatives of type 9 protein export. Using this system, we showed that the lipoprotein export signal, which has recently been found in other species in the phylum Bacteroidota, is also functional in FimA, and that a proton motive force inhibitor can affect type 9 protein export. Collectively, our conditional protein expression method is useful for screening inhibitors of virulence factors, and may be used to investigate the role of proteins essential to bacterial survival in vivo.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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A conditional gene expression system in Porphyromonas gingivalis for study of the secretion mechanisms of lipoproteins and T9SS cargo proteins

Sasaki

Shoji

Sueyoshi

et al. 2023

Molecular Oral Microbiology

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“…One of the main causative agents of periodontitis is P. gingivalis, which expresses various adhesive components: fimbriae, gingipains, haemagglutinin, and lipopolysaccharide. An important role in the interaction of a bacterium with host cells is played by fimbriae, the so-called adhesive pili [1][2][3][4][5][6]. P. gingivalis fimbriae bind to the cellular a5B1 integrin, which mediates bacterial adhesion to host cells [1,[7][8][9].…”

Section: Introductionmentioning

confidence: 99%

The Relationship between Mutations in Gene-Specific Domains of Salivary Fibronectin (cFn) and Dynamin-2 (Dynm-2) and the Development of Porphyromonas gingivalis-Initiated Periodontitis

Oleinik

Гончаренко

2022

JMP

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Periodontitis is a chronic inflammatory disease characterized by the destruction of the supporting structures of the teeth. Its high prevalence and negative effects on quality of life make it one of the current problems in dentistry. Porphyromonas gingivalis (P. gingivalis) is the predominant periodontal pathogen that expresses a number of virulence factors involved in the pathogenesis of periodontitis. P. gingivalis fimbriae are a critical factor in the interaction between the organism and the host tissue. They promote both bacterial adhesion and invasion into the target sites. Fimbriae are capable of binding to human saliva components, extracellular matrix proteins, and commensal bacteria, as well as firmly binding to the cellular integrin α5β1. After attachment to α5β1-integrin, P. gingivalis is captured by cellular pseudopodia, which makes invagination through an actin-mediated pathway possible. It has been proven that the invagination event also requires the participation of the host cell dynamin, actin fibers, microtubules and lipid rafts. Work has emerged investigating mutations in the proline-rich terminal domain (PRD) and their impact on disease development. Salivary antimicrobial peptides are early protective factors against microbial attack. Of great interest is fibronectin (FN) as the main competitor of P. gingivalis fimbriae. The FN can interact with cells in three different regions: the central cell-binding domain (CCBD), the COOH terminal heparin-binding domain (Hep2), and the type III connecting segment (IIICS), including the CS1 region (Yamada, 1991). CCBD is the major cell-adhesion domain of FN and contains an Arg–Gly–Asp (RGD) motif that is recognized by members of the cell adhesion receptor integrin family, including a5b1, which is the primary FN receptor in many cell types. The work focuses on identifying the relationship between the development of periodontitis and the presence of mutations in the adhesion domains of salivary proteins such as cellular fibronectin (cFN) and dynamin-2 (DYNM2).

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