Transport and intracellular metabolism of major feed compounds by ruminal bacteria: the potential for metabolic manipulation

McSweeney, Christopher S.; Mackie, RI; White, BA

doi:10.1071/ar9940731

Cited by 13 publications

(5 citation statements)

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“…The P H 2 up to 2.2 · 10 −2 bar may thermodynamically inhibit hydrogenase catalyzed NADH oxidation in rumen bacteria (e.g., Van Lingen et al, 2016 ). In this thermodynamic state, the metabolism oxidizes NADH back to NAD + by generating more reduced fermentation products (e.g., Counotte and Prins, 1981 ; McSweeney et al, 1994 ; Fischbach and Sonnenburg, 2011 ). This explains why increased proportions of propionate at the expense of acetate were observed, and why lactate and ethanol appeared in response to feeding (Figure 2 ).…”

Section: Discussionmentioning

confidence: 99%

Diurnal Dynamics of Gaseous and Dissolved Metabolites and Microbiota Composition in the Bovine Rumen

et al. 2017

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Diurnal patterns of ruminal fermentation metabolites and microbial communities are not commonly assessed when investigating variation in ruminal CH4 production. The aims of this study were to monitor diurnal patterns of: (i) gaseous and dissolved metabolite concentrations in the bovine rumen, (ii) H2 and CH4 emitted, and (iii) the rumen microbiota. Furthermore, the effect of dietary inclusion of linseed oil on these patterns was assessed. Four rumen cannulated multiparous cows were used in a cross-over design with two 17 days periods and two dietary treatments: a control diet and a linseed oil supplemented diet [40% maize silage, 30% grass silage, 30% concentrate on dry matter (DM) basis for both diets; fat contents of 33 vs. 56 g/kg of DM]. On day 11, rumen contents were sampled for 10 h after morning feeding to profile gaseous and dissolved metabolite concentrations and microbiota composition. H2 and CH4 emission (mass per unit of time) was measured in respiration chambers from day 13 to 17. A 100-fold increase in ruminal H2 partial pressure (contribution to the total pressure of rumen headspace gases) was observed at 0.5 h after feeding. This peak was followed by a decline to basal level. Qualitatively similar patterns after feeding were also observed for H2 and CH4 emission, ethanol and lactate concentrations, and propionate molar proportion, although the opposite pattern was seen for acetate molar proportion. Associated with these patterns, a temporal biphasic change in the microbial composition was observed as based on 16S ribosomal RNA with certain taxa specifically associated with each phase. Bacterial concentrations (log10 16S ribosomal RNA gene copies based) were affected by time, and were increased by linseed oil supplementation. Archaeal concentrations (log10 16S ribosomal RNA gene copies based) tended to be affected by time and were not affected by diet, despite linseed oil supplementation decreasing CH4 emission, tending to decrease the partial pressure of CH4, and tending to increase propionate molar proportion. Linseed oil supplementation affected microbiota composition, and was most associated with an uncultivated Bacteroidales taxon. In summary, our findings support the importance of diurnal dynamics for the understanding of VFA, H2, and CH4 production.

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Section: Discussionmentioning

confidence: 99%

Diurnal Dynamics of Gaseous and Dissolved Metabolites and Microbiota Composition in the Bovine Rumen

et al. 2017

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“…O sistema GDH prevalece em condições de elevadas concentrações de N-NH 3 , sem gasto de ATP. Entretanto, o GS-GOGAT atua efetivamente em baixas concentrações de N-NH 3 , mas com gasto de ATP (Erfle et al, 1977;McSweeney et al, 1993;Morrison & Mackie, 1996). Considerando que a produção de ATP no rúmen é relativamente baixa, as vias de assimilação dos nutrientes podem exercer grande efeito sobre a eficiência microbiana.…”

Section: Introductionunclassified

Eficiência microbiana e parâmetros ruminais em bovinos alimentados com dietas à base de volumosos tropicais

Cabral¹,

Filho²,

Detmann³

et al. 2008

R. Bras. Zootec.

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RESUMO -Objetivou-se com este trabalho determinar o fluxo de compostos nitrogenados no abomaso, a degradação ruminal dos carboidratos totais e da matéria orgânica, a eficiência microbiana, a concentração de N-NH 3 e o pH ruminal em bovinos alimentados com dietas à base de silagem de milho, silagem de capim-elefante ou feno de capim-tifton 85.Foram utilizados seis bovinos fistulados no rúmen e abomaso em um delineamento quadrado latino 3 × 3 duplo. Os fluxos de matéria seca da digesta e da matéria seca microbiana no abomaso foram determinados a partir da utilização de fibra em detergente neutro indigestível e das bases purinas como indicador microbiano, respectivamente. As concentrações de NH 3 e o pH ruminal foram determinados antes e 2, 4 e 8 horas após a alimentação. As dietas à base de silagem de milho e de feno de capim-tifton 85 proporcionaram os maiores fluxos de compostos nitrogenados no abomaso. As bactérias isoladas do rúmen apresentaram em média 8,89% de N total e 18,40 para a relação N-RNA:N-total. A dieta à base de silagem de milho promoveu maior degradação ruminal da matéria orgânica (2,96 kg/dia) e dos carboidratos totais (3,07 kg/dia) e maior fluxo de massa microbiana para o abomaso (788,28 g/dia). As dietas à base de feno de capim-tifton 85 e de silagem Key Words: corn silage, elephantgrass, microbial protein, ruminal pH and NH 3 , Tifton hay IntroduçãoO rúmen-retículo é o principal local de digestão dos componentes dietéticos nos ruminantes, ação exercida pela numerosa e diversa população microbiana deste compartimento (Van Soest, 1994).Considerando que o crescimento microbiano no rúmen está associado à capacidade digestiva do animal e influencia

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“…A fundamental understanding of microbial ecology and relationships to ruminant physiology is essential for successful manipulation of ruminal microflora and subsequent improvement in animal production since rumen microflora play important roles in the nutrient and energy uptake of the host (25). Hence, principles such as niche occupancy, selective pressure, adaptation, and interactions among populations (42) as well as the kinetics of substrate utilization (18) have to be taken into account when evaluating the ruminal microflora and host interactions.…”

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confidence: 99%

Correlation of Particular Bacterial PCR-Denaturing Gradient Gel Electrophoresis Patterns with Bovine Ruminal Fermentation Parameters and Feed Efficiency Traits

Hernandez-Sanabria

Guan

Goonewardene

et al. 2010

Appl Environ Microbiol

104

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The influence of rumen microbial structure and functions on host physiology remains poorly understood. This study aimed to investigate the interaction between the ruminal microflora and the host by correlating bacterial diversity with fermentation measurements and feed efficiency traits, including dry matter intake, feed conversion ratio, average daily gain, and residual feed intake, using culture-independent methods. Universal bacterial partial 16S rRNA gene products were amplified from ruminal fluid collected from 58 steers raised under a low-energy diet and were subjected to PCR-denaturing gradient gel electrophoresis (DGGE) analysis. Multivariate statistical analysis was used to relate specific PCR-DGGE bands to various feed efficiency traits and metabolites. Analysis of volatile fatty acid profiles showed that butyrate was positively correlated with daily dry matter intake (P < 0.05) and tended to have higher concentration in inefficient animals (P ‫؍‬ 0.10), while isovalerate was associated with residual feed intake (P < 0.05). Our results suggest that particular bacteria and their metabolism in the rumen may contribute to differences in host feed efficiency under a low-energy diet. This is the first study correlating PCR-DGGE bands representing specific bacteria to metabolites in the bovine rumen and to host feed efficiency traits.

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Transport and intracellular metabolism of major feed compounds by ruminal bacteria: the potential for metabolic manipulation

Cited by 13 publications

References 133 publications

Diurnal Dynamics of Gaseous and Dissolved Metabolites and Microbiota Composition in the Bovine Rumen

Diurnal Dynamics of Gaseous and Dissolved Metabolites and Microbiota Composition in the Bovine Rumen

Eficiência microbiana e parâmetros ruminais em bovinos alimentados com dietas à base de volumosos tropicais

Correlation of Particular Bacterial PCR-Denaturing Gradient Gel Electrophoresis Patterns with Bovine Ruminal Fermentation Parameters and Feed Efficiency Traits

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