Transport and consumption of oxygen and methane in different habitats of the Håkon Mosby Mud Volcano (HMMV)

Felden, Janine; Wenzhöfer, Frank; Feseker, Tomas; Boetius, Antje

doi:10.4319/lo.2010.55.6.2366

Cited by 96 publications

(94 citation statements)

References 40 publications

(143 reference statements)

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“…ANME-2c was predominantly found at sites inhabited by vesicomyid clams [hydrate ridge calyptogena site (HRC), JAP, and KO] (20,21,32). ANME-3 was also widespread but dominated only samples from cold-temperature, vigorously emitting methane seeps, such as HMMV in the Barents sea (33) and the REGAB pockmark (KO) in the Congo Basin (21). In addition, the relative abundance of ANME-3 correlated with that of SEEP-SRB4 (R spearman = 0.79, p spearman < 0.001), indicating that those two organisms may form AOM aggregates in those environments.…”

Section: Resultsmentioning

confidence: 99%

Global dispersion and local diversification of the methane seep microbiome

Ruff

Biddle

Teske

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

244

236

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Methane seeps are widespread seafloor ecosystems shaped by the emission of gas from seabed reservoirs. The microorganisms inhabiting methane seeps transform the chemical energy in methane to products that sustain rich benthic communities around the gas leaks. Despite the biogeochemical relevance of microbial methane removal at seeps, the global diversity and dispersion of seep microbiota remain unknown. Here we determined the microbial diversity and community structure of 23 globally distributed methane seeps and compared these to the microbial communities of 54 other seafloor ecosystems, including sulfate-methane transition zones, hydrothermal vents, coastal sediments, and deepsea surface and subsurface sediments. We found that methane seep communities show moderate levels of microbial richness compared with other seafloor ecosystems and harbor distinct bacterial and archaeal taxa with cosmopolitan distribution and key biogeochemical functions. The high relative sequence abundance of ANME (anaerobic methanotrophic archaea), as well as aerobic Methylococcales, sulfate-reducing Desulfobacterales, and sulfide-oxidizing Thiotrichales, matches the most favorable microbial metabolisms at methane seeps in terms of substrate supply and distinguishes the seep microbiome from other seafloor microbiomes. The key functional taxa varied in relative sequence abundance between different seeps due to the environmental factors, sediment depth and seafloor temperature. The degree of endemism of the methane seep microbiome suggests a high local diversification in these heterogeneous but long-lived ecosystems. Our results indicate that the seep microbiome is structured according to metacommunity processes and that few cosmopolitan microbial taxa mediate the bulk of methane oxidation, with global relevance to methane emission in the ocean.anaerobic methane oxidation | ANME | deep-sea seafloor ecosystems | microbial community ecology | environmental selection A microbiome is defined as the microbial community and its genomic diversity associated with a particular ecosystem or habitat, such as soil (1) or the human gut (2). A key question in the study of microbiomes concerns the identification of assembly rules that govern microbial community structure and community function (3). Sampling efforts on local to global scales have been used to determine the key drivers of microbial assembly, which include processes such as dispersal, ecological drift, environmental selection, and diversification (3, 4). Major processes shaping the microbial diversity landscape involve environmental selection of organisms according to their traits, niche preferences, biological interactions, and coevolution with hosts (5, 6). In turn, recent findings suggest that fluctuations of key microbial taxa reflect the dynamics of important biogeochemical processes (7).Insights into environmental microbiomes have tremendously improved with the use of next-generation sequencing methods and global databases, which have advanced microbial ecology from the identificati...

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Section: Resultsmentioning

confidence: 99%

Global dispersion and local diversification of the methane seep microbiome

Ruff

Biddle

Teske

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

244

236

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“…6). Only relatively low SR and AOM rates were measured at the vents, as compared to other methane-rich hydrothermal vents and seeps that are not impacted by CO 2 leakage (Biddle et al, 2011;Felden et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Generally, microbial life is very limited in the vented sediments. Notably, with ∼ 5 × 10 −8 mol, oxygen consumption (Table 3) of reduced compounds in surface porewaters (Felden et al, 2010;Ristova et al, 2012). Typically, methane seeps at similar water depths show oxygen consumption rates of 10-100 × 10 −8 mol.…”

Section: Discussionmentioning

confidence: 99%

“…Methane, dissolved inorganic carbon (DIC), and ammonium fluxes were determined with a cylindrical benthic chamber module as previously described (Felden et al, 2010) operated by the ROV QUEST. Briefly, the stirred chamber (radius 9.5 cm) enclosed a seafloor area of 284 cm 2 together with 10-15 cm (equivalent to 4-6 L) of overlying bottom water.…”

Section: Benthic Chamber Measurementsmentioning

confidence: 99%

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Saturated CO<sub>2</sub> inhibits microbial processes in CO<sub>2</sub>-vented deep-sea sediments

et al. 2013

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Abstract. This study focused on biogeochemical processes and microbial activity in sediments of a natural deep-sea CO2 seepage area (Yonaguni Knoll IV hydrothermal system, Japan). The aim was to assess the influence of the geochemical conditions occurring in highly acidic and CO2 saturated sediments on sulfate reduction (SR) and anaerobic methane oxidation (AOM). Porewater chemistry was investigated from retrieved sediment cores and in situ by microsensor profiling. The sites sampled around a sediment-hosted hydrothermal CO2 vent were very heterogeneous in porewater chemistry, indicating a complex leakage pattern. Near the vents, droplets of liquid CO2 were observed emanating from the sediments, and the pH reached approximately 4.5 in a sediment depth > 6 cm, as determined in situ by microsensors. Methane and sulfate co-occurred in most sediment samples from the vicinity of the vents down to a depth of 3 m. However, SR and AOM were restricted to the upper 7–15 cm below seafloor, although neither temperature, low pH, nor the availability of methane and sulfate could be limiting microbial activity. We argue that the extremely high subsurface concentrations of dissolved CO2 (1000–1700 mM), which disrupt the cellular pH homeostasis, and lead to end-product inhibition. This limits life to the surface sediment horizons above the liquid CO2 phase, where less extreme conditions prevail. Our results may have to be taken into consideration in assessing the consequences of deep-sea CO2 sequestration on benthic element cycling and on the local ecosystem state.

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“…Irrespective of those limits, diving time, and thus the number of cores per diving operation, may become reduced at greater depths because of increased decompression obligations for the divers and so the maximum depth for optimal sampling may be shallower than 30 m. That said, a number of studies (Dias et al 2008;Felden et al 2010;Van Gaever et al 2010;Jackson et al 2017), have used push cores mounted to remotely operated vehicles (ROVs) to sample sediments at depths in excess of a thousand meters.…”

Section: Discussion and Recommendationsmentioning

confidence: 99%

The influence of coring method on the preservation of sedimentary and biogeochemical features when sampling soft‐bottom, shallow coastal environments

Mogg

Attard

Ståhl

et al. 2017

Limnology & Ocean Methods

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It is frequently assumed that taking samples of soft marine sediments using surface-based gravity coring equipment causes minimal disruption to their sedimentological, biogeochemical, and biological condition. This assumption was evaluated by examining the potential disturbances caused when obtaining softsediment samples either by SCUBA or Craib-coring, comparing sediment oxygen microprofiles, benthic oxygen flux rates and sediment solid phase analyses (chlorophyll a (Chl a), organic carbon, and porosity) between both methods and against reference values measured in situ by benthic lander. The two sampling methodologies were tested in shallow coastal environments on the west coast of Scotland and generally the results obtained from cores collected using SCUBA exhibited values closest to those observed in situ. Oxygen penetration depth was significantly shallower in cores obtained by Craib-corer compared with the SCUBA cores. Craib cores also produced higher oxygen uptake rates which could be caused by greater levels of sediment disturbance during sampling. In addition, more homogenous levels of Chl a in the top 1 cm of the Craib cores, compared with the SCUBA samples, may indicate either resuspension or compression during gravity coring. Using SCUBA for shallow-water soft-sediment sampling permits steady and controlled coretube insertion and extraction, and more measured retrieval of the cores to the surface; this probably accounts for the observed differences. Whereas benthic lander-based in situ measurement would be the preferred method for analyzing sediment parameters in detail in this type of environment, SCUBA-based sampling offers a more accurate alternative to surface-based gravity coring.The ability to extract sediment samples that retain their in situ sedimentological, biogeochemical, and biological conditions is central to many marine and limnic investigations. The widespread demand for such observations has resulted in the application of a large array of different sediment sampling techniques (Blomqvist 1991). However, comparative assessments of these techniques and how well they represent in situ conditions are limited and focus mainly on the biological parameters measured in the samples (Blomqvist 1991). Glud et al. (1994Glud et al. ( , 1999 found shallower oxygen penetration depths (OPD), steeper oxygen concentration gradients and higher sediment oxygen uptake rates near the sediment surface in deep sea sediment cores incubated on board ship (ex situ) compared to the OPD and oxygen uptake rates recorded by an in situ benthic lander (Tengberg et al. 1995). Enhanced sediment nutrient concentrations have also been found in porewaters extracted ex situ (by core slicing and centrifugation) compared with data retrieved in situ using passive-equilibration "peepers" (Jahnke et al. 1989;Hammond et al. 1996;Aller et al. 1998). Hall et al. (2007 found sharp sub-surface maxima of the ratio of dissolved organic carbon (DOC) to dissolved organic nitrogen (DON) and carbohydrates in deep-sea retrieved sed...

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Transport and consumption of oxygen and methane in different habitats of the Håkon Mosby Mud Volcano (HMMV)

Cited by 96 publications

References 40 publications

Global dispersion and local diversification of the methane seep microbiome

Global dispersion and local diversification of the methane seep microbiome

Saturated CO<sub>2</sub> inhibits microbial processes in CO<sub>2</sub>-vented deep-sea sediments

The influence of coring method on the preservation of sedimentary and biogeochemical features when sampling soft‐bottom, shallow coastal environments

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Transport and consumption of oxygen and methane in different habitats of the Håkon Mosby Mud Volcano (HMMV)

Cited by 96 publications

References 40 publications

Global dispersion and local diversification of the methane seep microbiome

Global dispersion and local diversification of the methane seep microbiome

Saturated CO&lt;sub&gt;2&lt;/sub&gt; inhibits microbial processes in CO&lt;sub&gt;2&lt;/sub&gt;-vented deep-sea sediments

The influence of coring method on the preservation of sedimentary and biogeochemical features when sampling soft‐bottom, shallow coastal environments

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Saturated CO<sub>2</sub> inhibits microbial processes in CO<sub>2</sub>-vented deep-sea sediments