Transponder-Induced Sarcoma in the Heterozygous p53+/- Mouse

Blanchard, Kerry T.; Barthel, Curt H.; French, John E.; Holden, Henry E.; Moretz, R. C.; Pack, Franklin D; Tennant, Raymond W.; Stoll, Raymond E.

doi:10.1177/019262339902700505

Cited by 64 publications

(45 citation statements)

References 13 publications

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“…An advantage of transponder use is that they can be kept within tissues collected from the animals to keep tracking data from the original animal. In contrast to some long-term studies, 20,25,26 no tumours were detected in association with the transponders in 20-week-old C57BL/6J mice in the present study.…”

Section: Postmortem Analysiscontrasting

confidence: 99%

Identification methods in newborn C57BL/6 mice: a developmental and behavioural evaluation

et al. 2010

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The use of group-housed rodents in many fields of biomedical research imposes a need to identify individuals in a cage. Few studies have been designed to assess possible negative effects of identification methods of newborn mice on their development and wellbeing. In the present study, three different identification methods were applied to newborn C57BL/6J mice on postnatal day ( pnd) 5 (toe clipping, toe tattoo ink puncture and subcutaneous implantation of a small transponder). All identification methods used proved to be effective for long-term marking of individual animals. Newborn mice showed the least reaction to toe clipping followed by toe tattoo ink puncture and transponder implantation was the most distressful individual identification procedure in newborn mice. Importantly, clipped toe tissue proved to be enough for genotyping purposes. No overall consistent differences in somatic and neurological reflex development during the postnatal period were shown as a result of the newborn individual identification procedures used. Further, none of the methods interfered significantly with the adult animals' general normal behaviour (e.g. ability to move, grasp, climb) and sensory -motor functions as assessed with a simplified SHIRPA battery of tests, as well as Rotarod and Elevated Plus Maze tests. Postmortem thymus and adrenal gland weights gave no indication of chronic stress as a consequence of the identification method. We conclude that toe clipping might even be advisable in newborn mice at a very young age, when genotyping is needed. Toe tattoo ink puncture is also a good identification method for newborn mice and transponder implantation should only be used in older newborns or applied at weaning.

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Section: Postmortem Analysiscontrasting

confidence: 99%

Identification methods in newborn C57BL/6 mice: a developmental and behavioural evaluation

et al. 2010

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“…9,11 Once encapsulation is complete, a microchip transponder is expected to function safely; however, transponder-associated neoplasia has been described in laboratory mice, laboratory rats, pet dogs, an Egyptian fruit bat, a degu, and a feathertail glider. 3,4,10,[12][13][14][15] The majority of transponder-associated tumors have been mesenchymal in origin, and the mechanism of carcinogenicity is thought to be foreign body-induced tumorigenesis. 4 In this report, we describe the findings of tumors associated with implanted microchip transponders in 2 adult female Damaraland mole rats.…”

Section: Discussionmentioning

confidence: 99%

Neoplasia and Granulomas Surrounding Microchip Transponders in Damaraland Mole Rats (Cryptomys damarensis)

et al. 2010

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Damaraland mole rats (Cryptomys damarensis) are among the longest-living rodents, with a maximum longevity of approximately 16 years. As one of the few mammals termed eusocial, these animals have been used in behavioral, genetic, metabolic, and physiologic research at the University of Connecticut since 1997. For individual identification at 3 to 4 months of age, mole rats were subcutaneously implanted with microchip transponders (11 mm in length) in the dorsal cervical region. In 2007, 2 of the 90 implanted adults, 10-year-old and 9-year-old females, developed subcutaneous masses at the site of the implant. Histopathological and immunohistochemical examinations revealed amelanotic melanoma and fibrosarcoma, respectively, with metastasis of the amelanotic melanoma. In 2008, a total of 3 adult males were castrated as part of a sex behavior study; 3 months later, all 3 castrated males developed subcutaneous masses around their implants, whereas none of the noncastrated males had masses. After an additional 9 months, these masses were found to be granulomas. To the authors' knowledge, this is the first report of neoplasia in this species. Both the tumors and the granulomas surrounded the microchip transponder.

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“…Five out of eight articles reported that 0.8 -4.1% of laboratory mice and rats developed malignant tumours around or adjacent to the implanted microchips. 22 In one of these eight studies, the investigators used a genetically modified line ( p53þ/2 mice) that was prone to develop cancer, and 10.2% of the mice developed tumours 5 and in several cases these tumours also metastasized. The tumours generally occurred in the second year of the studies, in middle aged or older animals.…”

Section: Microchip Transpondermentioning

confidence: 99%

Report of the Federation of European Laboratory Animal Science Associations Working Group on animal identification

Dahlborn

Bugnon

Nevalainen³

et al. 2013

Lab Anim

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The primary aim of this report is to assist scientists in selecting more reliable/suitable identification (ID) methods for their studies. This is especially true for genetically altered (GA) animals where individual identification is strictly necessary to link samples, research design and genotype. The aim of this Federation of European Laboratory Animal Science Associations working group was to provide an update of the methods used to identify rodents in different situations and to assess their implications for animal welfare. ID procedures are an indispensable prerequisite for conducting good science but the degree of invasiveness differs between the different methods; therefore, one needs to make a good ethical evaluation of the method chosen. Based on the scientific literature the advantages and disadvantages of various methods have been presented comprehensively and this report is intended as a practical guide for researchers. New upcoming methods have been included next to the traditional techniques. Ideally, an ID method should provide reliable identification, be technically easy to apply and not inflict adverse effects on animals while taking into account the type of research. There is no gold standard method because each situation is unique; however, more studies are needed to better evaluate ID systems and the desirable introduction of new and modern approaches will need to be assessed by detailed scientific evaluation.

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Transponder-Induced Sarcoma in the Heterozygous p53+/- Mouse

Cited by 64 publications

References 13 publications

Identification methods in newborn C57BL/6 mice: a developmental and behavioural evaluation

Identification methods in newborn C57BL/6 mice: a developmental and behavioural evaluation

Neoplasia and Granulomas Surrounding Microchip Transponders in Damaraland Mole Rats (Cryptomys damarensis)

Report of the Federation of European Laboratory Animal Science Associations Working Group on animal identification

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