Transplanted xenogenic bone marrow stem cells survive and generate new bone formation in the posterolateral lumbar spine of non-immunosuppressed rabbits

Park, Jong-Beom; Lee, Jin Kyung; Park, Eun‐Young; Park, Eun-Ae; Riew, K. Daniel; Rhee, Seung Koo

doi:10.1007/s00586-008-0784-9

Cited by 23 publications

(15 citation statements)

References 30 publications

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“…First, murine BMSCs, unlike their human equivalent, lack major histocompatibility complex (MHC) class II expression [13], and second, T-cell inhibition by BMSCs requires cell contact in mice but is mediated by soluble factors in humans [14,15]. In any event, reports of successful xenotransplantation with BMSCs from various species continue to appear frequently; for example, rat BMSC for bone formation in rabbit [16], human BMSC for spinal cord injury in rat [17,18], and human BMSC for bone formation in mice [19].…”

Section: Stem Cells and Developmentmentioning

confidence: 99%

Allogeneic and Xenogeneic Transplantation of Adipose-Derived Stem Cells in Immunocompetent Recipients Without Immunosuppressants

Lin

Lue

2012

Stem Cells and Development

181

152

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Section: Stem Cells and Developmentmentioning

confidence: 99%

Allogeneic and Xenogeneic Transplantation of Adipose-Derived Stem Cells in Immunocompetent Recipients Without Immunosuppressants

Lin

Lue

2012

Stem Cells and Development

181

152

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“…Healing was measurable as early as six weeks following in-vivo implantation, and the quantity of new bone formed was significantly greater than untreated and control groups [55]. Similarly, experiments by Kim et al showed that xenogenic bone marrow stem cells were capable of surviving and contributing to bone repair in rabbits where defects in the lumbar spine existed [56]. The stem cells used in this study were combined with a compression resistant synthetic scaffold before implantation.…”

Section: In-vivo Modelsmentioning

confidence: 87%

“…Several in-vivo stem cell studies have shown the benefit of applying implanted stem cells to the repair of a tissue [55][56][57]. Zhang et al showed that bone marrow stem cells, when combined in-vitro with a fibrin extracellular glue, were capable of forming new bone in areas of alveolar bone defects in rats [55].…”

Section: In-vivo Modelsmentioning

confidence: 98%

“…The stem cells used in this study were combined with a compression resistant synthetic scaffold before implantation. Using Y-chromosome staining (used to monitor the viability of the male rabbit-derived cells), viability of the implanted cells was demonstrated between 1 to 6 months following implantation [56]. Additionally, the synthetic matrix created for implantation was eventually resorbed in the defect, and the production of new, mature bone containing both osteoblasts and osteocytes was demonstrated histologically [56].…”

Section: In-vivo Modelsmentioning

confidence: 99%

“…Using Y-chromosome staining (used to monitor the viability of the male rabbit-derived cells), viability of the implanted cells was demonstrated between 1 to 6 months following implantation [56]. Additionally, the synthetic matrix created for implantation was eventually resorbed in the defect, and the production of new, mature bone containing both osteoblasts and osteocytes was demonstrated histologically [56]. Finally, a study by Minamide et al successfully showed that bone marrow stem cells cultured in-vitro and seeded in a collagen-I gel with hydroxyapatite was capable of producing new bone formation in rabbits containing spinal bone defects [57].…”

Section: In-vivo Modelsmentioning

confidence: 99%

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Stem Cell-Based Tissue Engineering for Bone Repair

Damaraju

Duncan

2014

Tissue Engineering

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Culturing cells in 3D scaffolds can help model a physiological process. The property of the substrate used for such scaffolds has been shown to modify and determine stem cell lineage. Using this knowledge, in-vitro 3D stem cell culture models with ex-vivo bone tissue investigations can offer insight and inspiration for the development of novel therapies for bone defects. Although many different scaffolds have been created for bone tissue repair, in situ cell level mechanics are not always given consideration as the main design target. Overall, the ideal tissue engineering solution to bone regeneration would incorporate cells of osteogenic potential into a synthetic bone scaffold in order to reduce the need for external factors added such as drugs or growth factors. Attention to the mechanical aspects of the bone to be studied as well as the cells to be placed within the scaffold is fundamental. In this chapter, we will explore studies investigating the role of cell communication in bone mechanosensing, including the roles of different bone cells in the process of bone adaptation and repair, and the use of this knowledge in creating a novel tissue engineering strategy for the repair of acute bone defects.

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Regeneration enhanced in critical‐sized bone defects using bone‐specific extracellular matrix protein

et al. 2020

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Extracellular matrix (ECM) products have the potential to improve cellular attachment and promote tissue‐specific development by mimicking the native cellular niche. In this study, the therapeutic efficacy of an ECM substratum produced by bone marrow stem cells (BM‐MSCs) to promote bone regeneration in vitro and in vivo were evaluated. Fluorescence‐activated cell sorting analysis and phenotypic expression were employed to characterize the in vitro BM‐MSC response to bone marrow specific ECM (BM‐ECM). BM‐ECM encouraged cell proliferation and stemness maintenance. The efficacy of BM‐ECM as an adjuvant in promoting bone regeneration was evaluated in an orthotopic, segmental critical‐sized bone defect in the rat femur over 8 weeks. The groups evaluated were either untreated (negative control); packed with calcium phosphate granules or granules+BM‐ECM free protein and stabilized by collagenous membrane. Bone regeneration in vivo was analyzed using microcomputed tomography and histology. in vivo results demonstrated improvements in mineralization, osteogenesis, and tissue infiltration (114 ± 15% increase) in the BM‐ECM complex group from 4 to 8 weeks compared to mineral granules only (45 ± 21% increase). Histological observations suggested direct apposition of early bone after 4 weeks and mineral consolidation after 8 weeks implantation for the group supplemented with BM‐ECM. Significant osteoid formation and greater functional bone formation (polar moment of inertia was 71 ± 0.2 mm4 with BM‐ECM supplementation compared to 48 ± 0.2 mm4 in untreated defects) validated in vivo indicated support of osteoconductivity and increased defect site cellularity. In conclusion, these results suggest that BM‐ECM free protein is potentially a therapeutic supplement for stemness maintenance and sustaining osteogenesis.

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Transplanted xenogenic bone marrow stem cells survive and generate new bone formation in the posterolateral lumbar spine of non-immunosuppressed rabbits

Abstract: Bone marrow stem cells (BMSCs) are pluripotent cells that have been used to facilitate bone repair because of their capability of differentiating into osteo-

Cited by 23 publications

References 30 publications

Allogeneic and Xenogeneic Transplantation of Adipose-Derived Stem Cells in Immunocompetent Recipients Without Immunosuppressants

Allogeneic and Xenogeneic Transplantation of Adipose-Derived Stem Cells in Immunocompetent Recipients Without Immunosuppressants

Stem Cell-Based Tissue Engineering for Bone Repair

Regeneration enhanced in critical‐sized bone defects using bone‐specific extracellular matrix protein

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