Transplanted human multipotent stromal cells reduce acute tongue fibrosis in rats

Vahabzadeh‐Hagh, Andrew M.; Goel, Alexander N.; Frederick, John W.; Berke, Gerald S.; Long, Jennifer L.

doi:10.1002/lio2.202

Cited by 4 publications

(7 citation statements)

References 35 publications

(40 reference statements)

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“…A rat partial glossectomy injury model, previously developed to mimic the fibrosis and muscle atrophy that follows head and neck cancer treatment, was used. 9 The study design (Figure 1A) included injection volumes ranging from 50 to 300 μL.…”

Section: Resultsmentioning

confidence: 99%

“…Male Sprague Dawley rats weighing between 225-250 g (approximately 3 months old) underwent partial glossectomy injury, as previously established. 9 Briefly, animals were anesthetized with isoflurane, and buprenorphine was delivered subcutaneously for pain management. The tongue was retracted with a 4–0 silk suture.…”

Section: Methodsmentioning

confidence: 99%

“…The animals were then monitored for 5 days postoperatively; the rats were provided with a soft diet for the duration of the study period. Scar formation at the site of injury occurred over the following 2 weeks, 9 at which time point SKM was injected directly into the tongue.…”

Section: Methodsmentioning

confidence: 99%

“…Fluorescently pre-labelled SKM was injected directly into the site of injury, two weeks following a partial glossectomy injury. Injection volumes of 50, 100, 200, and 300 μL were tested (n=2 animals per volume); this range was determined based on SKM injections in other skeletal muscle injury models 21 and cell injections in the rat tongue 9 . Animals were euthanized and tongues were harvested 1 week following injection; cryosectioned and stained with DAPI nuclear counterstain for tissue localization.…”

Section: Methodsmentioning

confidence: 99%

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Immunomodulatory Extracellular Matrix Hydrogel Mitigates Scar Formation in a Model of Tongue Fibrosis

Zelus

Panduro

Alperin

et al. 2022

Preprint

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Background: While head and neck cancer treatment regimens, including surgical resection, irradiation, and chemotherapy, are effective at removing tumors, they lead to muscle atrophy, denervation, and fibrosis, contributing to the pathogenesis of tongue dysphagia - difficulty swallowing. Current standard of care is ineffective; we propose an alternative approach utilizing an acellular and minimally invasive biomaterial to preserve muscle content and reduce fibrosis of the tongue after injury. Here, we investigate a decellularized extracellular matrix hydrogel for the treatment of tongue fibrosis in a partial glossectomy injury model. Methods: Skeletal muscle extracellular matrix (SKM) hydrogel was fabricated by decellularizing porcine skeletal muscle tissue through established protocols. A partial glossectomy injury in the rat was used as a model of tongue fibrois, and SKM hydrogels along with saline controls were injected to the site of scarring two weeks after injury. Tissues were harvested at 3 and 7 days post-injection for gene expression analysis of immune and myogenic pathways, and at 4 weeks post-injection to evaluate histomorphological changes in skeletal muscle and scar formation. Results: SKM hydrogel reduced scar formation and improved muscle fiber cross-sectional area in the region of injury compared to saline controls. SKM upregulated pro-regenerative immune response while downregulating pro-inflammatory response and further promoted angiogenic gene expression. Conclusion: This study demonstrates the immunomodulatory and tissue-regenerative capacity of an acellular and minimally invasive biomaterial in a rodent model of tongue fibrosis.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Immunomodulatory Extracellular Matrix Hydrogel Mitigates Scar Formation in a Model of Tongue Fibrosis

Zelus

Panduro

Alperin

et al. 2022

Preprint

Self Cite

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“…At 12 wk, the human MDSC group exhibited greater bioluminescence than immediately following injection, and increased weight gain compared to mice receiving saline injections only. Separately, Vahabzadeh et al[ 24 ] performed partial glossectomy in 18 athymic rats. After two weeks for scar formation to mimic a tumor resection scenario, animals were treated with either low- or high-dose mesenchymal stem cell (MSC) or saline control.…”

Section: Stem Cell Therapies For Tongue Dysfunctionmentioning

confidence: 99%

Stem cell treatments for oropharyngeal dysphagia: Rationale, benefits, and challenges

Tran¹,

Juarez²,

Long³

2020

WJSC

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Dysphagia, defined as difficulty swallowing, is a common symptom negatively impacting millions of adults annually. Estimated prevalence ranges from 14 to 33 percent in those over age 65 to over 70 percent in a nursing home setting. The elderly, those with neurodegenerative diseases, head and neck cancer patients, and those with autoimmune conditions such as Sjögren's syndrome are disproportionately affected. Oropharyngeal dysphagia refers specifically to difficulty in initiating a swallow due to dysfunction at or above the upper esophageal sphincter, and represents a large proportion of dysphagia cases. Current treatments are limited and are often ineffective. Stem cell therapy is a new and novel advancement that may fill a much-needed role in our treatment regimen. Here, we review the current literature regarding stem cell treatments for oropharyngeal dysphagia. Topics discussed include tissue regeneration advancements as a whole and translation of these principles into research surrounding tongue dysfunction, xerostomia, cricopharyngeal dysfunction, and finally an overview of the challenges and future directions for investigation. Although this field of study remains in its early stages, initial promising results show potential for the use of stem cell-based therapies to treat oropharyngeal dysphagia and warrant further research.

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Locally injected bone marrow-derived mesenchymal stem cells reverts the histopathological changes in the tongue of carbimazole-induced hypothyroidism of male rats

Mahmoud,

Badawy,

Mohammed

et al. 2024

Archives of Oral Biology

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Transplanted human multipotent stromal cells reduce acute tongue fibrosis in rats

Cited by 4 publications

References 35 publications

Immunomodulatory Extracellular Matrix Hydrogel Mitigates Scar Formation in a Model of Tongue Fibrosis

Immunomodulatory Extracellular Matrix Hydrogel Mitigates Scar Formation in a Model of Tongue Fibrosis

Stem cell treatments for oropharyngeal dysphagia: Rationale, benefits, and challenges

Locally injected bone marrow-derived mesenchymal stem cells reverts the histopathological changes in the tongue of carbimazole-induced hypothyroidism of male rats

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