Transplanted Bone Marrow derived Cells Differentiated toTooth, Bone and Connective Tissues in Mice

Abstract

Teeth are important structures for masticatory and phonetic purposes. Loss of teeth decreases these functions leading to impaired quality of life. Missing teeth replaced by tooth regeneration may be possible with emerging advances in stem cell biology and tissue engineering. Recent investigations have demonstrated that bone marrow derived cells (BMDC) can differentiate into cells other than blood cells. In the present study, the ability of BMDC to differentiate into tooth forming tissues was investigated using bone marrow transplantation model. BMDC from green fluorescent protein (GFP) transgenic mice were transplanted into 8-week old, C57BL/ 6 immunocompromised mice, which underwent 10-Gy whole body lethal irradiation. Immunohistochemical analysis revealed that bone marrow cells are positive to GFP, confirming successful bone marrow transplantation. Diffusedly GFP-positive cells were observed within the dental pulp of mouse incisor. GFP-positive cells in the dental pulp have arborescent processes resembling dendritic cell-like cells. Some odontoblast-like cells were also positive to GFP. Cells positive to GFP were observed in the cervical loop region and periodontal ligament. Langerhans cells in the oral epithelium, stromal fibroblasts, blood vessels and osteoclasts were also positive to GFP. These results indicate that BMDC have the ability to differentiate into tooth, bone and connective tissues.

The Ability of Transplanted Bone Marrow-Derived Cells to Differentiate into Parenchymal Cells of Salivary Glands

“…This coincides with previous findings, which showed that BMDCs have the possibility of migrating into many organs presenting microchimerism after transplantation in irradiated mice 7,9,12,[19][20][21] . Salivary gland tissues are composed of various types of cells, which originate from mesenchymal stem cells (MSCs) 22) .…”

“…confirmed many times in our previous studies 9,19) . One month after BMT, HE staining of recipient mice revealed there was almost no apparent damage in the salivary gland parenchyma (Fig.…”

Pathological Examination of Experimentally Induced Periodontal Polyp in Mice

“…Kaneko et al 13) also showed that the periodontal ligament fibroblasts were derived from the bone marrow. On the other hand Tsujigiwa et al 14) showed that bone marrow mesenchymal cells could also differentiate into odontogenic cells. Hence, further analysis to clarify the nature of cells making up the periodontal membrane polyp is necessary.…”

Establishment of Experimental Periapical Inflammatory Lesions in Mice

“…The study also revealed that the mesenchymal stem cells differentiated into a variety of cell types in their local destination [12][13][14][15] . Therefore, the present experiment system together with GFP bone marrow transplant mice established by Tsujigiwa et al 10) would contribute greatly to the understanding of the mechanism in the formation of chronic apical periodontitis.…”