Transplantation of the Stromal Vascular Fraction (SVF) Mitigates Severe Radiation-Induced Skin Injury

Yu, Daojiang; Zhang, Shuaijun; Mo, Wei; Jiang, Ziying; Wang, Min; An, Lu; Wang, Youyou; Liu, Yulong; Jiang, Sheng; Wu, Ailing; Cao, Jianping; Zhang, Shuyu

doi:10.1667/rade-20-00156.1

Cited by 12 publications

(15 citation statements)

References 48 publications

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“…Previous studies have found that MSC(AT)s or MSC(AT)s-Exo could promote soft tissue healing in various complex conditions. Yu et al suggested that MSC(AT)s could mitigate severe radiation-induced skin injury [28]. Moreover, MSC(AT)s-Exo was also reported to accelerate cutaneous wound healing in a diabetic wound by promoting vascularization [29] or by inducing miR-128-3p/SIRT1-mediated autophagy [30].…”

Section: Discussionmentioning

confidence: 99%

Exosomes Derived from Adipose Tissue-Derived Mesenchymal Stromal Cells Prevent Medication-Related Osteonecrosis of the Jaw through IL-1RA

Zheng

Dong

Wang

et al. 2023

IJMS

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Medication-related osteonecrosis of the jaw (MRONJ) is a severe disease with unclear pathogenesis. Adipose tissue-derived mesenchymal stromal cells (MSC(AT)s) serve as a special source for cell therapy. Herein, we explored whether exosomes (Exo) derived from MSC(AT)s promote primary gingival wound healing and prevent MRONJ. An MRONJ mice model was constructed using zoledronate (Zol) administration and tooth extraction. Exosomes were collected from the conditioned medium (CM) of MSC(AT)s (MSC(AT)s-Exo) and locally administered into the tooth sockets. Interleukin-1 receptor antagonist (IL-1RA)-siRNA was used to knock down the expression of IL-1RA in MSC(AT)s-Exo. Clinical observations, micro-computed tomography (microCT), and histological analysis were used to evaluate the therapeutic effects in vivo. In addition, the effect of exosomes on the biological behavior of human gingival fibroblasts (HGFs) was evaluated in vitro. MSC(AT)s-Exo accelerated primary gingival wound healing and bone regeneration in tooth sockets and prevented MRONJ. Moreover, MSC(AT)s-Exo increased IL-1RA expression and decreased interleukin-1 beta (IL-1β) and tumor necrosis factor-α (TNF-α) expression in the gingival tissue. The sequent rescue assay showed that the effects of preventing MRONJ in vivo and improving the migration and collagen synthesis abilities of zoledronate-affected HGFs in vitro were partially impaired in the IL-1RA-deficient exosome group. Our results indicated that MSC(AT)s-Exo might prevent the onset of MRONJ via an IL-1RA-mediated anti-inflammatory effect in the gingiva wound and improve the migration and collagen synthesis abilities of HGFs.

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Section: Discussionmentioning

confidence: 99%

Exosomes Derived from Adipose Tissue-Derived Mesenchymal Stromal Cells Prevent Medication-Related Osteonecrosis of the Jaw through IL-1RA

Zheng

Dong

Wang

et al. 2023

IJMS

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“…Patient cooperation and psychological factors may also impact treatment outcomes. Differences in autologous fat extraction sites and SVF preparation methods could affect treatment e cacy [30][31][32]. The relationship between injection frequency, doses, and SVF effectiveness requires further investigation to identify the optimal injection volume or saturation point.…”

Section: Discussionmentioning

confidence: 99%

Autologous adipose-derived vascular stromal component injection offers a safe and effective method for treating knee osteoarthritis: A one-year double-blind, randomized controlled clinical trial

Lu,

Xu,

et al. 2023

Preprint

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Background: Currently, conservative treatment for knee osteoarthritis (KOA) has limited efficacy, and autologous adipose-derived stromal vascular fraction (SVF) knee injections as a novel treatment approach are receiving widespread attention. Our study aimed to explore the efficacy and safety of SVF treatment for KOA patients. Methods: This double-blind, randomized controlled trial recruited unilateral KOA patients from the Rehabilitation Departments of the First, Second, and Third Affiliated Hospitals of Gannan Medical University. Sixty-six unilateral KOA participants were randomly divided into three groups for conventional treatment, SVF treatment, and a combination of SVF and conventional treatment. We compared the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores, Visual Analogue Scale (VAS) scores, range of motion (ROM) of the knee, cartilage degeneration situation, and the incidence, nature, and severity of adverse events after treatment at 7 days, 1 month, 6 months, and 12 months. Results:A total of 62 patients completed the follow-up. There were no significant baseline differences among the groups. Our results demonstrated that, compared to baseline values, average VAS and WOMAC scores significantly decreased, while ROM significantly increased in the SVF and combination treatment groups during the 12-month follow-up, with a significant difference when compared to the control group (P< 0.05). Cartilage regeneration was observed in the combination treatment group at the 12-month follow-up (P < 0.05). No serious adverse events were observed during the 12-month follow-up, and no significant difference was noted in the incidence of complications among the three groups (P > 0.05). Conclusion:A single SVF injection demonstrates good safety, no serious adverse reactions, and can achieve better therapeutic effects when combined with conventional treatment, which is worth further investigation and promotion in clinical practice. Trial registration Chinese Clinical Trial Registry (ChiCTR2300074894). First trial registration in the format 18/08/2023

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“…While grafts can be successful to treat CRI, they are also at risk for failure with wound recurrence if areas were exposed to doses of radiation resulting in necrosis. Other interventions for CRI can include use of investigational mesenchymal, 56,57 or adipose stem/ stromal cells, 58 as well as adipose-derived stromal vascular fraction, 59 and/ or growth factors, potentially utilized with more traditional modalities of dermal constructs, skin grafts, flap, and/ or amputations. 45 There remains a need for clinical trials repurposing current therapies for similar injuries, for novel MCM research in animal models, and adaptation of bench to bedside advancements of research.…”

Section: Cutaneous Radiation Injury (Cri) (C Iddins)mentioning

confidence: 99%

Overlapping Science in Radiation and Sulfur Mustard Exposures of Skin and Lung: Consideration of Models, Mechanisms, Organ Systems, and Medical Countermeasures: Overlapping science in radiation and sulfur mustard injuries to lung and skin

Satyamitra,

Andres,

Bergmann

et al. 2023

Disaster med. public health prep.

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Purpose: To summarize presentations and discussions from the 2022 trans-agency workshop titled “Overlapping Science in Radiation and Sulfur Mustard (SM) Exposures of Skin and Lung: Consideration of Models, Mechanisms, Organ Systems, and Medical Countermeasures.” Methods: Summary on topics includes: 1) an overview of the radiation and chemical countermeasure development programs and missions; 2) regulatory and industry perspectives for drugs and devices; 3) pathophysiology of skin and lung following radiation or SM exposure; 4) mechanisms of action/targets, biomarkers of injury; and 5) animal models that simulate anticipated clinical responses. Results: There are striking similarities between injuries caused by radiation and SM exposures. Primary outcomes from both types of exposure include acute injuries, while late complications comprise chronic inflammation, oxidative stress, and vascular dysfunction, which can culminate in fibrosis in both skin and lung organ systems. This workshop brought together academic and industrial researchers, medical practitioners, U.S. Government program officials and regulators to discuss lung-, and skin-specific animal models and biomarkers, novel pathways of injury and recovery, and paths to licensure for products to address radiation or SM injuries. Conclusions: Regular communications between the radiological and chemical injury research communities can enhance the state-of-the-science, provide a unique perspective on novel therapeutic strategies, and improve overall U.S. Government emergency preparedness.

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Transplantation of the Stromal Vascular Fraction (SVF) Mitigates Severe Radiation-Induced Skin Injury

Cited by 12 publications

References 48 publications

Exosomes Derived from Adipose Tissue-Derived Mesenchymal Stromal Cells Prevent Medication-Related Osteonecrosis of the Jaw through IL-1RA

Exosomes Derived from Adipose Tissue-Derived Mesenchymal Stromal Cells Prevent Medication-Related Osteonecrosis of the Jaw through IL-1RA

Autologous adipose-derived vascular stromal component injection offers a safe and effective method for treating knee osteoarthritis: A one-year double-blind, randomized controlled clinical trial

Overlapping Science in Radiation and Sulfur Mustard Exposures of Skin and Lung: Consideration of Models, Mechanisms, Organ Systems, and Medical Countermeasures: Overlapping science in radiation and sulfur mustard injuries to lung and skin

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