Transplantation of the decellularized tracheal allograft in animal model (rabbit)

Ershadi, Reza; Rahim, Mohammad Bagher; Jahany, Siavash; Rakei, Siamak

doi:10.1016/j.asjsur.2017.02.007

Cited by 12 publications

(23 citation statements)

References 23 publications

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“…The immunological rejection of tracheal grafts has been the biggest obstacle to the success of tracheal replacement, and its immunogenicity is mainly derived from the epithelial and mucosal lamina propria. 25,27,28 Decellularization technology can effectively dissolve epithelial and mucosal cells in natural trachea through detergents and enzymes, remove the immunogenicity of the matrix, and eliminate the need for immunosuppressive agents to reduce rejection after surgery. 9,10 Studies have shown that nonchondral cells, such as those in the epithelium, interstitium, and muscle of the tracheal matrix of rabbits, are completely eliminated by seven DEM cycles, and MHC-II antigens are removed.…”

Section: Discussionmentioning

confidence: 99%

Rapid preparation of decellularized trachea as a 3D scaffold for organ engineering

et al. 2020

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Objective: To shorten the preparation time of rabbit decellularized tracheal matrix through a modified detergent-enzymatic method with higher concentration of DNase (50 kU/mL), providing an experimental and theoretical basis for clinical decellularization technology. Methods: The control group was a natural trachea, and the experimental group was a tracheal matrix subjected to two and four decellularization cycles. The performance of each group of samples was evaluated by histology and immunohistochemical staining, scanning electron microscopy, biomechanical property testing, inoculation and cytotoxicity tests, and allograft experiments. Results: The results showed that the nuclei of the nonchondral areas of the tracheal stroma were essentially completely removed and MHC-I and MHC-II antigens were removed after two decellularization cycles. Histological staining and scanning electron microscopy showed that the extracellular matrix was retained and the basement membrane was intact. Cell inoculation and proliferation tests confirmed that the acellular tracheal matrix had good biocompatibility, and the proliferation capacity of bone mesenchymal stem cells on the matrix was increased in the experimental group compared with the control group ( p < 0.05). Histological staining and CD68 molecular marker analysis after the allograft experiment showed that the inflammatory response of the acellular tracheal matrix was weak and the infiltration of surrounding macrophages was reduced. Conclusion: A modified detergent-enzymatic method with an increased DNase (50 kU/mL) concentration requires only two cycles (4 days) to obtain a decellularized rabbit tracheal matrix with a short preparation time, good biocompatibility, suitable mechanical properties, and reduced preparation cost.

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Section: Discussionmentioning

confidence: 99%

Rapid preparation of decellularized trachea as a 3D scaffold for organ engineering

et al. 2020

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“…Due to the complexity of lung tissue and the need for instant functionality of used implants, a lung's dECM requires recellularization with epithelial and endothelial cells via cell infusion and bioreactors for ex vivo generation, maturation, and maintenance of the so-called bioartificial lungs. In vivo implantation of these organs in rats yields anastomosis, but long-term success is still to be achieved [143,144].…”

Section: Tissues Related To Respiratory Systemmentioning

confidence: 99%

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

Mendibil

Ruiz-Hernández

Retegi-Carrión

et al. 2020

IJMS

185

187

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The extracellular matrix (ECM) is a complex network with multiple functions, including specific functions during tissue regeneration. Precisely, the properties of the ECM have been thoroughly used in tissue engineering and regenerative medicine research, aiming to restore the function of damaged or dysfunctional tissues. Tissue decellularization is gaining momentum as a technique to obtain potentially implantable decellularized extracellular matrix (dECM) with well-preserved key components. Interestingly, the tissue-specific dECM is becoming a feasible option to carry out regenerative medicine research, with multiple advantages compared to other approaches. This review provides an overview of the most common methods used to obtain the dECM and summarizes the strategies adopted to decellularize specific tissues, aiming to provide a helpful guide for future research development.

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“…The updates in creating tracheal grafts have been demonstrated through protocol improvements in physical decellularization, chemical decellularization, and bioreactor methods in the past five years. [73][74][75][76][77] These protocol advancements have also been seen in other organ systems using animal-based models such as esophagus (murine-rat), 78 lung (ovine), 79 cartilage (porcine), 80 uterus (ovine and leporine), 81,82 bladder (leporine), 83 pericardium (bovine), 84 submillimeter vasculature (murine-rat), 85 and intestine (murine-mouse) 86 model tissues. Protocol advancement in the field of vasculature has been especially important given this is often viewed as a challenge due to the size and difficulty of reseeding these grafts with endothelial cells.…”

Section: History and Development Of Decellularization For Bioenginementioning

confidence: 85%

“…Institution of a protocol to accomplish ingrowth of multiple cell types may be the key to creating implant‐ready grafts. The updates in creating tracheal grafts have been demonstrated through protocol improvements in physical decellularization, chemical decellularization, and bioreactor methods in the past five years 73–77 . These protocol advancements have also been seen in other organ systems using animal‐based models such as esophagus (murine‐rat), 78 lung (ovine), 79 cartilage (porcine), 80 uterus (ovine and leporine), 81,82 bladder (leporine), 83 pericardium (bovine), 84 submillimeter vasculature (murine‐rat), 85 and intestine (murine‐mouse) 86 model tissues.…”

Section: Extracellular Structural Matrix Functionsmentioning

confidence: 99%

Decellularized scaffolds for tissue engineering: Current status and future perspective

Rajab¹,

O’Malley

Tchantchaleishvili

2020

Artificial Organs

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Based on Organ Procurement and Transplantation Network data as of December 2019, more than 113 000 patients require an organ transplantation, yet, over the course of this past year, only 36 000 patients received a transplant. This discrepancy between those that need a donor organ and those that receive one remains one of medicine’s biggest challenges. Multiple solutions, both biologic and artificial, have been proposed to mitigate this difference. One of the most promising approaches to generate bioartificial organs for transplantation involves re‐seeding decellularized scaffolds with appropriate cells. Decellularization involves physical, chemical, or biological methods that typically require intimate contact of various decellularization solutions with each cell. Consequently, conventional submersion decellularization has been limited to simple tissues such as heart valves. The invention of perfusion decellularization was a breakthrough that allowed the generation of tissue‐engineered scaffolds from tissues with higher structural organization and entire organs. Such scaffolds are composed of a myriad of extracellular matrix (ECM) components that include collagen, elastin, proteoglycans, and glycoproteins. Together, these components allow the scaffolds to fulfill specialized functions, such as structural functions as well as biological functions including the regulation of cellular processes and extracellular molecules. These specialized functions of decellularized scaffolds can increasingly be harnessed for applications in tissue engineering.

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Transplantation of the decellularized tracheal allograft in animal model (rabbit)

Cited by 12 publications

References 23 publications

Rapid preparation of decellularized trachea as a 3D scaffold for organ engineering

Rapid preparation of decellularized trachea as a 3D scaffold for organ engineering

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

Decellularized scaffolds for tissue engineering: Current status and future perspective

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