Transplantation of oligodendrocyte progenitor cells into the rat retina: Extensive myelination of retinal ganglion cell axons

Morphological and Functional Characterization of Predifferentiation of Myelinating Glia-Like Cells from Human Bone Marrow Stromal Cells Through Activation of F3/Notch Signaling in Mouse Retina

“…OPCs and NPCs have the ability to differentiate into OLs and form myelin after transplantation of these cells into rat retina [34,35]. Given that F3/Notch signaling promotes the maturation of OPCs into OLs [5], we were interested in investigating whether F3 could play a similar role in promoting the maturation of the predifferentiated MGLCs in vivo.…”

“…Thus, the rodent retina offers several advantages for transplantation experiments with myelinating glial cells [35]. Notably, myelination of the intraretinal axon segments occurs under a variety of pathological conditions [48,49] or after transplantation of OPCs into the rat retina [34] or neural precursor cells into the retina of young postnatal mice [35]. In agreement with these previous observations, our immunofluorescence and EM results demonstrate that these predifferentiated hBMSCs induced by F3/contactin, which we have called MGLCs, have the potential to form axoglial junctions and myelin sheaths (Fig.…”

Peculiar and typical oligodendrocytes are involved in an uneven myelination pattern during the ontogeny of the lizard visual pathway

“…The myelination pattern during the ontogeny of the visual pathway has been studied in different mammalian species such as opossum, rat, and human (Friede and Hu, 1967a,b;Nakayama, 1967;Skoff et al, 1980), in amphibians such as Xenopus (Cima and Grant, 1982), and in fishes such as zebrafish (Brösamle and Halpern, 2002), but studies of the pattern remain completely lacking in reptiles. Intraretinal myelination is absent in most mammals, although transplantation methods have shown that their intraretinal ganglion cell axons are capable of being myelinated (Laeng et al, 1996;Ader et al, 2000). The arrest of the oligodendrocyte migration at the retinal optic nerve junctions seems to be responsible for this absence of intraretinal myelination (Morcos and Chang-Ling, 2000).…”

Terminal differentiation of myelin-forming oligodendrocytes depends on the transcription factor Sox10

“…Neural stem cells were prepared from the spinal cords of 12.5-dpc Sox10 lacZ /Sox10 lacZ embryos and age-matched control littermates, expanded as neurospheres, and injected as singlecell suspensions in equal numbers into the retinas of 3-to 9-day-old C57BL/6J recipient mice (Ader et al 2000). Intraretinal segments of ganglion cell axons are normally unmyelinated, but can be experimentally myelinated by intraretinal transplantation of myelinogenic cells (Laeng et al 1996;Ader et al 2000). A fraction of intraretinally grafted stem cells from wild-type donors differentiated into oligodendrocytes in the vicinity of the nerve fiber layer and had produced massive amounts of myelin 1 mo after transplantation.…”