Transplantation of Neurons and Sheath Cells — A Tool for the Study of Regeneration

Aguayo, Albert J.; Richardson, Robert M.; David, Samuel; Benfey, M.

doi:10.1007/978-3-642-68632-0_7

Cited by 24 publications

(2 citation statements)

References 37 publications

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“…The work of Aguayo et al (1982) on CNS regeneration implicates a peripheral, non-neuronal cell as the source of factors "permissive" for axonal regeneration. One interesting possibility raised by the distribution of Rat-401-positive cells is that the Rat-401 antibody marks a cell type competent to support axon regeneration.…”

Section: Axonal Navigationmentioning

confidence: 99%

Identification of major cell classes in the developing mammalian nervous system

Hockfield¹,

McKay²

1985

J. Neurosci.

741

489

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A major difficulty in studying early developmental processes and testing hypotheses of possible cellular mechanisms of development has been the inability to reproducibly identify specific cell types. We have generated monoclonal antibodies that distinguish among major cell types present during mammalian neurogenesis. These antibodies have been used to analyze the development of cellular organization in the early nervous system. Monoclonal antibody Rat-401 identifies a transient radial glial cell in the embryonic rat central nervous system (CNS) that is temporally and spatially suited to guide neuronal migration. Rat-401 also identifies a peripheral non-neuronal cell that may establish axon routes from the CNS to the periphery. Monoclonal antibody Rat-202 recognizes an antigen present in early axons, their growth cones, and filopodia, and has allowed us to follow early axons and observe the structures they contact. Two other antibodies that recognize axons demonstrate antigenically distinct phases in axon development. In addition, we report a marker for another cell class present in the developing nervous system, the endothelial cells that give rise to the CNS vasculature.

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Section: Axonal Navigationmentioning

confidence: 99%

Identification of major cell classes in the developing mammalian nervous system

Hockfield¹,

McKay²

1985

J. Neurosci.

741

489

View full text Add to dashboard Cite

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“…738-740). These results have been confirmed by more recent experiments (3)(4)(5); many CNS neurons possess the ability to elongate axons for considerable distances when provided with a supportive environmente.g., a peripheral nerve graft (6).…”

mentioning

confidence: 99%

Identification of a peripheral nerve neurite growth-promoting activity by development and use of an in vitro bioassay.

Sandrock

Matthew²

1987

Proc. Natl. Acad. Sci. U.S.A.

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The effective regeneration of severed neuronal axons in the peripheral nerves of adult mammals may be explained by the presence of molecules in situ that promote the effective elongation of neurites. In mammals, severed neuronal axons of peripheral nerves regenerate readily, whereas axons of the central nervous system (CNS) fail to regrow effectively (1). Ramon y Cajal (2) was among the first to postulate that the rapid regeneration of peripheral axons was made possible by the presence of "neurotrophic substances" in the environment of elongating growth cones and that the relative inability of central axons to regenerate was due to the lack of these substances in CNS tissue, rather than an inherent defect of these neurons. This hypothesis was based, at least in part, on two observations: (i) regenerating dorsal root axons will elongate until the spinal cord is reached, where most of the fibers are "detained" or deflected to grow back down the root (ref. 2, p. 543), and (ii) sciatic nerve grafts placed into deeply cut adult rabbit cerebral cortex contained numerous axons originating from the surrounding white matter, "behaving in their growth, ramifications, orientation, energetic progress, etc., exactly like the sprouts of the central stump of a cut [peripheral] nerve" (ref. 2, pp. 738-740). These results have been confirmed by more recent experiments (3)(4)(5)

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Plasticity in the olfactory cortex: Age‐dependent effects of deafferentation

Friedman

Price

1986

J of Comparative Neurology

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In order to assess the role of input-target interactions in the development of olfactory cortex, the primary afferent fibers from the olfactory bulb to the superficial part of layer I of the cortex (layer Ia) were removed in developing and mature rats. After survival periods that vary from a few days to 2-6 months, changes were assessed in (1) the radial thickness of layer I, (2) the laminar distribution of intracortical associational fibers, which normally terminate in a deep part of layer I (layer Ib), and (3) the distribution of glia in layer I. The findings indicate that the lamination of fibers within layer I is not intrinsically prespecified, but gradually becomes "set" during the first month after birth. If the fibers from the olfactory bulb are removed, the dendrites of cortical cells are capable of accepting inputs from other fiber systems, depending on the maturational state of the dendrites and the ingrowing axons. Development of the abnormal inputs is associated with relatively normal dendritic growth, whereas lack of adequate input results in dendritic atrophy. Thus, after neonatal bulb ablation, the intracortical fibers occupy both superficial and deep parts of layer I, and a normal synaptic density is established throughout the layer. Layer I also develops to nearly its normal adult thickness, although the high density of glia that normally characterizes layer Ia is not apparent. With bulb ablation at progressively older ages (from postnatal day (P-) 3 to 21), the cortical associational fibers show progressively less extension into the denervated layer Ia. Layer I continues to grow, but not to the same extent as after P-1 ablations. In these experiments the glia distribution resembles the pattern present at the time of denervation. After adult olfactory bulb ablation, the long intracortical fibers extend very little into layer Ia, which undergoes pronounced shrinkage and becomes filled with a high concentration of glia. However, partial reinnervation of layer Ia is accomplished by the proliferation of a normally sparse native fiber system, which has been identified only with the Timm method. These results are interpreted as evidence that the normal development of lamination of afferent fibers to the olfactory cortex depends on axodendritic interaction during development.

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Transplantation of Neurons and Sheath Cells — A Tool for the Study of Regeneration

Cited by 24 publications

References 37 publications

Identification of major cell classes in the developing mammalian nervous system

Identification of major cell classes in the developing mammalian nervous system

Identification of a peripheral nerve neurite growth-promoting activity by development and use of an in vitro bioassay.

Plasticity in the olfactory cortex: Age‐dependent effects of deafferentation

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