Transplantation of neural stem cells overexpressing glia-derived neurotrophic factor promotes facial nerve regeneration

Shi, Yong; Zhou, Liang; Tian, Jie; Wang, Yang

doi:10.1080/00016480802468153

Cited by 45 publications

(44 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pre-clinical experiments have demonstrated that stem cells show promising results in differentiation into neuronal-like cells [226,228,232,233,240] and their secretion of growth factors [229,241,242]. Shi and colleagues [243] stated the potential of glia-derived neurotrophic factor expressing neural stem cells in the regeneration of facial nerve gap in rats.…”

Section: Cellular Systemsmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

View full text Add to dashboard Cite

Human adult peripheral nerve injuries are a high incidence clinical problem that greatly affects patients' quality of life. Although peripheral nervous system has intrinsic regenerative capacity, this occurs in an incomplete or poorly functional manner. When a nerve fiber loses its continuity with consequent damage of the basal lamina tubes, axon spontaneous regeneration is disorganized and mismatched. These phenomena translate in an inadequate nerve functional recovery and consequent musculoskeletal incapacity. Nerve grafts still remain the gold standard in peripheral injuries treatment. However, this approach contains its disadvantages such as the necessity of primary surgery to harvest the autografts, loss of a functional nerve, donor site morbidity and longer surgery procedures. Therefore, biomaterials and tissue engineering can provide efficient resources and alternatives to nerve injury repair not only by the development of biocompatible structures but also, introducing neurotrophic factors and cellular systems to stimulate optimum clinical outcome. In this chapter, a comprehensive state-of-the art picture of tissue-engineered nerve grafts scaffolds, their application in nerve regeneration along with latest advances in peripheral nerve repair and future perspectives will be discussed, including our own large experience in this field of knowledge.

show abstract

Section: Cellular Systemsmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

View full text Add to dashboard Cite

show abstract

“…Early intervention is preferable if denervated muscles have no opportunity to be reinnervated from regenerating peripheral axons or when reinnervation is delayed, as often occurs in brachial plexus injuries. [7][8][9][10][11][12]33 Some traditional methods, such as stimulation 5 and rehabilitation 6 are not effective in arresting denervated muscle atrophy. Prevention of muscle atrophy using fetal NSCs following denervation may be of therapeutic benefit in these cases.…”

Section: Discussionmentioning

confidence: 99%

“…12,34,35 Recent progress in the biology of NSCs has made it possible to routinely expand neural progenitor cells obtained from a small amount of fetal CNS tissue in vitro as floating cell aggregates called neurospheres. 33,34 Therefore, this may overcome the practical problems associated with fetal tissue transplantation.…”

Section: Discussionmentioning

confidence: 99%

Application of fetal neural stem cells transplantation in delaying denervated muscle atrophy in rats with peripheral nerve injury

Shen

et al. 2009

Microsurgery

View full text Add to dashboard Cite

Injury to peripheral nerves always results in progressive skeletal muscle atrophy and poor functional recovery. Previous studies have demonstrated that transplanting neural stem cells (NSCs) into peripheral nerve can differentiate into neurons and delay muscle atrophy. However, the mechanism was not very clear. In this study, we transplanted the fetal NSCs to the injured nerve and examined new formed neuromuscular junctions (NMJs) in the denervated muscle and arrest of muscle atrophy. In our study, two pregnant Fischer rats were used to harvest fetal NSCs, 70 rats were randomly divided into NSC-transplanted and control groups, five rats without surgery were used as the normal control. A volume of 5 microl culture media with or without fetal NSCs (5 x 10(6)) were transplanted into distal tibial nerve stump after the nerve was transected in two groups, respectively. Three, five, and seven months after denervation, the dry weight of gastrocnemius muscle was found significant heavier, and the fiber area was more retained in NSC-transplanted group comparing to the control group (P < 0.05). Neurons were found in the distal tibial nerves even 7 months after fetal NSCs transplantation. Newly formed NMJs were detected by immunohistochemistry. In addition, the results of electrophysiological analysis and retrograde tracing manifested that the neural pathway between muscle and differentiated neurons was integrity. In conclusions, our study demonstrated that fetal NSCs transplanted into peripheral nerves could differentiate into neurons and form functional NMJs with denervated muscle, which may be beneficial for the treatment of muscle atrophy after peripheral nerve injury.

show abstract

“…8 weeks after the implantation, comparable morphological rescues in mid-graft, including the number of axons and area of nerve fibers, were observed in the seeded implants and were comparable to autografts. Shi et al further investigated the combined effect of cell replacement therapy with sustained neurotrophic factor delivery [121]. NSCs and SCs were modified to express GDNF and infused into PLGA tubes followed by implantation into rats with facial nerve transections in two separate sets of experiments.…”

Section: Cell-carriers With Biodegradable Materialsmentioning

confidence: 99%

Carriers in Cell-Based Therapies for Neurological Disorders

Wong

Chan

2014

IJMS

View full text Add to dashboard Cite

There is a pressing need for long-term neuroprotective and neuroregenerative therapies to promote full function recovery of injuries in the human nervous system resulting from trauma, stroke or degenerative diseases. Although cell-based therapies are promising in supporting repair and regeneration, direct introduction to the injury site is plagued by problems such as low transplanted cell survival rate, limited graft integration, immunorejection, and tumor formation. Neural tissue engineering offers an integrative and multifaceted approach to tackle these complex neurological disorders. Synergistic therapeutic effects can be obtained from combining customized biomaterial scaffolds with cell-based therapies. Current scaffold-facilitated cell transplantation strategies aim to achieve structural and functional rescue via offering a three-dimensional permissive and instructive environment for sustainable neuroactive factor production for prolonged periods and/or cell replacement at the target site. In this review, we intend to highlight important considerations in biomaterial selection and to review major biodegradable or non-biodegradable scaffolds used for cell transplantation to the central and peripheral nervous system in preclinical and clinical trials. Expanded knowledge in biomaterial properties and their prolonged interaction with transplanted and host cells have greatly expanded the possibilities for designing suitable carrier systems and the potential of cell therapies in the nervous system.

show abstract

Transplantation of neural stem cells overexpressing glia-derived neurotrophic factor promotes facial nerve regeneration

Cited by 45 publications

References 20 publications

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Application of fetal neural stem cells transplantation in delaying denervated muscle atrophy in rats with peripheral nerve injury

Carriers in Cell-Based Therapies for Neurological Disorders

Contact Info

Product

Resources

About