Transplantation of mouse ovarian tissue: Comparison of the transplantation sites

Youm, Hye Won; Lee, Jung Ryeol; Lee, Jaewang; Jee, Byung Chul; Suh, Chang Suk; Kim, Seok Hyun

doi:10.1016/j.theriogenology.2014.11.026

Cited by 35 publications

(22 citation statements)

References 30 publications

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“…One of the most important disadvantages of ovarian tissue transplantation is related to ischemia, which can cause follicular loss (Schubert et al 2008). There are many published reports aimed at improving ovarian tissue transplantation efficiency by reducing ischemia in the transplanted tissue (Israely et al 2006;Behbahanian et al 2013;Labied et al 2013;Henry et al 2015;Youm et al 2015). One of the factors affecting angiogenesis which prevents ischemia in transplanted tissue is site of transplantation (Behbahanian et al 2013;Youm et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…There are many published reports aimed at improving ovarian tissue transplantation efficiency by reducing ischemia in the transplanted tissue (Israely et al 2006;Behbahanian et al 2013;Labied et al 2013;Henry et al 2015;Youm et al 2015). One of the factors affecting angiogenesis which prevents ischemia in transplanted tissue is site of transplantation (Behbahanian et al 2013;Youm et al 2015). In our previous research, gluteal muscle was shown to be an intriguing site for whole ovarian transplantation (Behbahanian et al 2013).…”

Section: Discussionmentioning

confidence: 99%

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Histological evaluation of the effect of VEGF on auto-transplanted mouse ovaries

Zand-vakili

Eimani

Golkar‐Narenji

et al. 2016

Animal Cells and Systems

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One of the most important factors affecting survival rate of ovarian follicles during transplantation period is proper vascular development. The objective of the study was to evaluate the effect of vascular endothelial growth factor (VEGF) on auto-transplanted ovarian tissue. Twenty-one-dayold female mice (n = 30) transplanted as control group and 21-day-old female mice (n = 40) were divided into 4 groups that were treated with 0.5, 1, 2 and 4 µg/mL of VEGF directly injected to auto-transplanted ovarian tissue. Twenty-one days after transplantation, mice were treated with 7.5 IU pregnant mare serum gonadotropin and human chorionic gonadotropin. Transplanted ovaries were removed and sections were prepared from transplanted tissues for staining. The most effective dosage of VEGF on transplanted tissue was determined over H&E (hematoxylin and eosin) staining results. Slides were compared using TUNEL staining and CD31 assay for the most effective dosage. The percentages of preantral and antral follicles were not significantly different between transplanted group with 4 µg/mL VEGF and non-transplanted group. Lower apoptotic areas and higher CD31 expression were observed in transplanted ovaries treated with 4 µg/mL VEGF when compared to transplanted ovaries without VEGF treatment. VEGF positively affects the quality of ovarian tissue during transplantation. Survival rate of follicles and follicular development has improved with the effect of VEGF. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Histological evaluation of the effect of VEGF on auto-transplanted mouse ovaries

Zand-vakili

Eimani

Golkar‐Narenji

et al. 2016

Animal Cells and Systems

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“…Then autotransplantation was performed as described in our previous report [22]. After analgesia of the mice, a small hole was made on their kidney capsules by using fine forceps and the vitrified-warmed ovaries were inserted under both of the kidney capsules.…”

Section: Methodsmentioning

confidence: 99%

Effects of Angiopoietin-2 on Transplanted Mouse Ovarian Tissue

et al. 2016

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Transplantation of ovarian tissue (OT) is currently the only clinical option to restore fertility with cryopreserved OT. However, follicle loss caused by ischemia and slow revascularization occurs in transplanted OT. To shorten the ischemic period and promote angiogenesis, some angiogenic factors have been used. Angiopoietin-2 (Ang2) is one of the major angiogenic factors and has been reported to promote blood vessels and increase vascular permeability in ischemic and/or hypoxic environment. This study was performed to investigate the effects of Ang2 on follicle integrity and revascularization of transplanted mouse OT. Five-week-old B6D2F1 female mice were divided into a control group and two Ang2 groups, followed by ovary collection and vitrification. After warming, the ovaries were autotransplanted into kidney capsules with/without Ang2 injection (50 or 500 ng/kg), and then the mice were sacrificed at days 2, 7, 21, and 42 after transplantation. A total 2,437 follicles in OT grafts were assessed for follicular density, integrity, and classification by using hematoxylin and eosin staining. Apoptosis and revascularization were evaluated by using TUNEL assay and CD31 immunohistochemistry, respectively. Serum follicle-stimulating hormone (FSH) levels were measured by using enzyme-linked immunosorbent assay. Both Ang2 groups showed remarkable increase in morphologically intact follicle ratio across all grafting durations except D21. The numbers of CD31(+) vessels were significantly increased in both Ang2 groups compared with the control group at all durations, except in the 50 ng Ang2 group at D42. However, the mean numbers of follicles of the grafts, apoptosis ratios, and serum FSH levels showed no significant differences among the groups. Our results show that Ang2 treatment significantly increased the intact follicle ratios and the number of blood vessels of the mouse OT grafts. However, further studies performed with large animal or human OT are necessary before clinical application for fertility preservation in cancer patients, and the reliability of the systemic effects of Ang2 should be verified.

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“…Therefore, reducing the ischemia time after transplantation is vital to the successful restoration of ovarian function (15). It could be deduced that GT is a good site for ovarian transplantation because it provides a supportive neovascularization environment in the graft site.…”

Section: Groupsmentioning

confidence: 99%

“…Because of rapid revascularization, ovarian transplantation under the kidney capsule site is common (13). Youm et al showed that the kidney capsule site, in comparison to BM and subcutaneous sites, is the optimal site for ovarian transplantation in the murine model (15). Soleimani et al found that ovarian graft into the BM had some advantages compared to the kidney capsule because of good angiogenesis conditions (11).…”

Section: Introductionmentioning

confidence: 99%

Effects of n-acetylcysteine on ovarian tissue autografted intogranulation tissue compared to back muscle in rats

Daneshpoya¹,

Karimipour²,

Zirakjavanmard³

et al. 2017

Turk J Med Sci

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Background/aim: Ovarian transplantation can preserve fertility in cancer patients. The aim of this study was to compare ovarian transplantation into granulation tissue (GT) versus back muscle (BM) sites and also to investigate the effects of N-acetylcysteine (NAC) as an antioxidant on ovarian survival in a rat autograft model. Materials and methods:Twenty-eight adult female rats were divided into four equal groups (n = 7 each) as follows: Group 1, ovarian tissue grafted into GT + saline (GT + saline); Group 2, ovarian tissue grafted into GT + NAC (GT + NAC); Group 3, ovarian tissue grafted into BM + saline (BM + saline); and Group 4, ovarian tissue grafted into BM + NAC (BM + NAC). After 28 days, serum concentrations of malondialdehyde (MDA), progesterone, and estradiol (E2) were measured. Ovarian follicle counts were performed from hematoxylin and eosin-stained slides. Furthermore, apoptosis was assessed by terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL).Results: There were no significant differences found in levels of MDA, progesterone, and E2 among the groups. The percentage of apoptotic granulosa cells was significantly (P < 0.05) lower in the ovarian tissue autografted in the GT + NAC group compared to the GT + saline and BM + NAC groups. In addition, the number of blood vessels formed in the ovarian tissue in the GT + NAC group was significantly higher than in the GT + saline and BM + NAC groups (P < 0.05 and P < 0.001 respectively). Conclusion:The granulation tissue site is a suitable candidate for ovarian graft in rats. Furthermore, NAC could not restore autografted ovarian functions.

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Transplantation of mouse ovarian tissue: Comparison of the transplantation sites

Cited by 35 publications

References 30 publications

Histological evaluation of the effect of VEGF on auto-transplanted mouse ovaries

Histological evaluation of the effect of VEGF on auto-transplanted mouse ovaries

Effects of Angiopoietin-2 on Transplanted Mouse Ovarian Tissue

Effects of n-acetylcysteine on ovarian tissue autografted intogranulation tissue compared to back muscle in rats

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