Transplantation of Mouse Eggs

Experiments on the transplantation of ova in mice

“…The transplantation operation was carried out similarly to the experiments made by Beatty (1951), except that a suitably constructed pipette was substituted fot the syringe. One end of the pipette was drawn out to form a micro-pipette (the edge of which had been melted to form a smooth surface), while the thick end was bent halfway along to form a right angle, and finished with a rubber tube which was kept in the mouth during the operation.…”

A Simple Method for Non-Surgical Blastocyst Transfer in Mice

“…Moreover, it offers by its simplicity advantages in teratological experiments where a certain loss of embryos can be predicted due to the action of the teratogen. The high 'take' rate seems to be of special value in fetal pharmacology experiments where strains with different suscepti- In earlier non-surgical trials in the mouse, Beatty (1951) (Briones & Beatty, 1954;Tarkowski, 1959 …”

“…The reviewer states that "it is obvious that a non-surgical technique for transferring eggs into the uterus is preferable to a surgical one". The poor results from the non-surgical transfers of mouse eggs reported by Beatty (1951) and Tarkowski (1959) have apparently made many researchers reluctant to try this method, though Vickery, Erickson & Bennett (1969) described a fair success with non-surgical egg transfer in the rat.…”

Comparison of Cornual Transfer via Laparotomy with Utero-Cervical Transfer of Cultured Preimplantation Rat Embryos

“…Cervical transfers have only rarely been described in the literature: in the mouse [5,6] and in the rat [7], The report by Vickery et al [7] in 1969 showed equal results by cervical or cornual transfer when carried out on days 4 or 5 of pseudopregnancy. Therefore, we intended to investigate and clarify the advantages of this technique in order to simplify the difficulties involved in cornual transfers.…”