Transplantation of mononuclear cells from bone marrow in a rat model of Huntington&amp;rsquo;s disease

Serrano, Teresa; Pierozan, Paula; Alberti, Esteban; Blanco, L. Solan; Bernal, Karelys de la Cuétara; González, María E.; Pavón, Nancy; Lorigados, Lourdes; Robinson-Agramonte, María A; Bergado, Jorge A.

doi:10.2147/jn.s118435

Cited by 3 publications

(4 citation statements)

References 31 publications

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“…39 Bone marrow medullary cavities contain several cell types with varied regenerative potential. 40 According to our findings, transplantation of both bone marrow ECs and MSCs can blunt detrimental effects of CTX within the ovarian tissue via an increase of normal follicles at different stages of development. In line with these changes, we indicated that the atretic changes were diminished after the direct transplantation of CD146 + MSCs and CD144 + ECs.…”

Section: Discussionmentioning

confidence: 58%

Comparison the therapeutic effects of bone marrow CD144⁺ endothelial cells and CD146⁺ mesenchymal stem cells in POF rats

et al. 2023

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Introduction: Premature ovarian insufficiency (POI) is a challenging issue in terms of reproduction biology. In this study, therapeutic properties of bone marrow CD146+ mesenchymal stem cells (MSCs) and CD144+ endothelial cells (ECs) were separately investigated in rats with POI. Methods: POI rats were classified into control POI, POI + CD146+ MSCs, and POI + CD144+ ECs groups. Enriched CD146+ MSCs and CD144+ ECs were directly injected into ovarian tissue (15 × 104 cells/10 μL) in relevant groups. After 4 weeks, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) levels were measured in blood samples. Ovarian tissues were collected and subjected to Hematoxylin-Eosin and Masson’s trichrome staining. The expression of angp-2, vegfr-2, smad-2, -4, -6, and tgf-β1 was studied using qRT-PCR analysis. Histopathological examination indicated an increased pattern of atretic follicles in the POI group related to the control rats (P<0.0001). Results: Data indicated that injection of POI + CD146+ MSCs and CD144+ ECs in POI rats reduced atretic follicles and increased the number of normal follicles (P<0.01). Along with these changes, the content of blue-colored collagen fibers was diminished after cell transplantation. Besides, cell transplantation in POI rats had the potential to reduce increased FSH, and LH levels (P<0.05). In contrast, E2 content was increased in POI + CD146+ MSCs and POI + CD144+ ECs groups compared to control POI rats, indicating restoration of follicular function. CD144+ (smad-2, and -4) and CD146+ (smad-6) cells altered the activity of genes belonging TGF-β signaling pathway. Unlike POI + CD146+ MSCs, aberrant angiogenesis properties were significantly down-regulated in POI + CD144+ ECs related to the control POI group (P<0.05). Conclusion: The transplantation of bone marrow CD146+ and CD144+ cells can lead to the restoration of ovarian tissue function in POI rats via modulating different mechanisms associated with angiogenesis and fibrosis

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Section: Discussionmentioning

confidence: 58%

Comparison the therapeutic effects of bone marrow CD144⁺ endothelial cells and CD146⁺ mesenchymal stem cells in POF rats

et al. 2023

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“…It is also likely that this occurs after the transplant as result of the trophic action of the transplanted cells. In addition, in previous studies, we demonstrated the histopathological changes that occur in the brain lesioned with quinolinic acid and transplanted with mBMCs, through morphological and immunohistochemical studies [ 36 , 37 ]. The observation using cresyl violet allowed a general inspection of the striatal morphology in the different experimental groups, showing the normal cell distribution in this region.…”

Section: Discussionmentioning

confidence: 97%

“…Lesioned and DMEM-treated animals showed evidence of intense degeneration, which was not seen in control and transplanted animals. Finally, they were studied through immunofluorescence study by NeuN and FJC staining [ 36 , 37 ] whereby NeuN, a neuronal marker, showed positive reactivity in the control and the transplanted groups. In contrast, these groups were negative for FJC.…”

Section: Discussionmentioning

confidence: 99%

Rotating and Neurochemical Activity of Rats Lesioned with Quinolinic Acid and Transplanted with Bone Marrow Mononuclear Cells

Sánchez

Fraguela

Lezcano

et al. 2018

Behavioral Sciences

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Huntington’s disease (HD) is an inherited, neurodegenerative disorder that results from the degeneration of striatal neurons, mainly GABAergic neurons. The study of neurochemical activity has provided reliable markers to explain motor disorders. To treat neurodegenerative diseases, stem cell transplants with bone marrow (BM) have been performed for several decades. In this work we determine the effect of mononuclear bone marrow cell (mBMC) transplantation on the rotational behavior and neurochemical activity in a model of Huntington’s disease in rats. Four experimental groups were organized: Group I: Control animals (n = 5); Group II: Lesion with quinolinic acid (QA) in the striatum (n = 5); Group III: Lesion with QA and transplant with mBMC (n = 5); Group IV: Lesion with QA and transplant with culture medium (Dulbecco’s modified Eagle’s medium (DMEM) injection) (n = 5). The rotational activity induced by D-amphetamine was evaluated and the concentration of the neurotransmitter amino acids (glutamate and GABA) was studied. The striatal cell transplantation decreases the rotations induced by D-amphetamine (p < 0.04, Wilcoxon matched pairs test) and improves the changes produced in the levels of neurotransmitters studied. This work suggests that the loss of GABAergic neurons in the brain of rats lesioned with AQ produces behavioral and neurochemical alterations that can be reversed with the use of bone marrow mononuclear cell transplants.

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“…This was centrifuged again for 10 min at 400G with brake at 22°C. The supernatant was then discarded and the collected cellular pellet was placed in a solution of 1× PBS with a total volume of about 1 cc [7], 10 µl were collected for cell counting, and 200 µl were taken for analysis by the flow cytometer. Using flow cytometry BD FACS ARIA III, these cells were characterized and identified for the cluster of differentiation (CD)-34+ markers.…”

Section: Isolation Of Bone Marrowmentioning

confidence: 99%

Comparative Dose of Intracarotid Autologous Bone Marrow Mononuclear Therapy in Chronic Ischemic Stroke in Rats

Makkiyah

Sadewo

Nurrizka

2021

Open Access Maced J Med Sci

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Research on chronic ischemic stroke is limited. One of the more promising approaches showing positive effects in the acute stage is mononuclear bone marrow cell therapy. This research may be the first which presents data about the optimum dose of bone marrow mononuclear cells (BM-MNCs) for chronic ischemic stroke in rats and discusses factors influencing recovery in the chronic stage. We performed temporary middle cerebral artery occlusion (MCAO) procedures on the rats which were then randomly assigned to one of two experimental groups in which they were given either low or high doses of autologous BM-MNCs (5 million or 10 million cells per kg body weight). Rat brains were fixed for HE, CD31, and doublecortin staining for analysis of the effects. Rat behavior was assessed weekly using the cylinder test and a modified neurological severity score (NSS) test. In the four weeks prior to administration of BM-MNC, cylinder test scores improved to near normal, and NSS test scores improved moderately. The infarct zone decreased significantly (p <0,01), there was an improvement in angiogenesis (p = 0.1590) and a significant improvement in neurogenesis (p <0,01). Reduction of the infarct zone was associated with a higher dose whereas both higher and lower doses were found to have a similar effect on improving angiogenesis, and neurogenesis. Recovery was superior after twelve weeks compared with the recovery assessment at eight weeks. In conclusion, a dose of 10 million cells was more effective than a dose of 5 million cells per kg body weight for reducing the infarct zone and ameliorating neurogenesis. There was an improvement of histopathological parameters associated with the longer infarct period.

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Transplantation of mononuclear cells from bone marrow in a rat model of Huntington’s disease

Cited by 3 publications

References 31 publications

Comparison the therapeutic effects of bone marrow CD144⁺ endothelial cells and CD146⁺ mesenchymal stem cells in POF rats

Comparison the therapeutic effects of bone marrow CD144⁺ endothelial cells and CD146⁺ mesenchymal stem cells in POF rats

Rotating and Neurochemical Activity of Rats Lesioned with Quinolinic Acid and Transplanted with Bone Marrow Mononuclear Cells

Comparative Dose of Intracarotid Autologous Bone Marrow Mononuclear Therapy in Chronic Ischemic Stroke in Rats

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Transplantation of mononuclear cells from bone marrow in a rat model of Huntington&rsquo;s disease

Cited by 3 publications

References 31 publications

Comparison the therapeutic effects of bone marrow CD144+ endothelial cells and CD146+ mesenchymal stem cells in POF rats

Comparison the therapeutic effects of bone marrow CD144+ endothelial cells and CD146+ mesenchymal stem cells in POF rats

Rotating and Neurochemical Activity of Rats Lesioned with Quinolinic Acid and Transplanted with Bone Marrow Mononuclear Cells

Comparative Dose of Intracarotid Autologous Bone Marrow Mononuclear Therapy in Chronic Ischemic Stroke in Rats

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Resources

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Transplantation of mononuclear cells from bone marrow in a rat model of Huntington’s disease

Comparison the therapeutic effects of bone marrow CD144⁺ endothelial cells and CD146⁺ mesenchymal stem cells in POF rats

Comparison the therapeutic effects of bone marrow CD144⁺ endothelial cells and CD146⁺ mesenchymal stem cells in POF rats