Transplantation of layer-by-layer assembled neural stem cells tethered with vascular endothelial growth factor reservoir promotes neurogenesis and angiogenesis after ischemic stroke in mice

Ge, Hongfei; Hu, Quan; Chen, Tunan; Yang, Yang; Zhang, Chao; Zhong, Jun; Yin, Yi; Jiang, Xuheng; Zhou, Xin; Wang, Shuhong; Hu, Rong; Li, Wenyan; Feng, Hua

doi:10.1016/j.apmt.2022.101548

Cited by 7 publications

(6 citation statements)

References 44 publications

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“…Ge et al [ 53 ] also applied a feasible method to encapsulate NSCs as a layered structure composed of gelatin and hyaluronic acid (HA) biomaterials in order to enhance the therapeutic potential of the NSCs for ischemic stroke. HA is a widely used polyanion that can easily bind to gelatin via electrospinning.…”

Section: Discussionmentioning

confidence: 99%

Biomaterial application strategies to enhance stem cell-based therapy for ischemic stroke

Satar¹,

Othman²,

Tan³

2022

World J Stem Cells

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BACKGROUND Ischemic stroke is a condition in which an occluded blood vessel interrupts blood flow to the brain and causes irreversible neuronal cell death. Transplantation of regenerative stem cells has been proposed as a novel therapy to restore damaged neural circuitry after ischemic stroke attack. However, limitations such as low cell survival rates after transplantation remain significant challenges to stem cell-based therapy for ischemic stroke in the clinical setting. In order to enhance the therapeutic efficacy of transplanted stem cells, several biomaterials have been developed to provide a supportable cellular microenvironment or functional modification on the stem cells to optimize their reparative roles in injured tissues or organs. AIM To discuss state-of-the-art functional biomaterials that could enhance the therapeutic potential of stem cell-based treatment for ischemic stroke and provide detailed insights into the mechanisms underlying these biomaterial approaches. METHODS The PubMed, Science Direct and Scopus literature databases were searched using the keywords of “biomaterial” and “ischemic stroke”. All topically-relevant articles were then screened to identify those with focused relevance to in vivo , in vitro and clinical studies related to “stem cells” OR “progenitor cells” OR “undifferentiated cells” published in English during the years of 2011 to 2022. The systematic search was conducted up to September 30, 2022. RESULTS A total of 19 articles matched all the inclusion criteria. The data contained within this collection of papers comprehensively represented 19 types of biomaterials applied on seven different types of stem/progenitor cells, namely mesenchymal stem cells, neural stem cells, induced pluripotent stem cells, neural progenitor cells, endothelial progenitor cells, neuroepithelial progenitor cells, and neuroblasts. The potential major benefits gained from the application of biomaterials in stem cell-based therapy were noted as induction of structural and functional modifications, increased stem cell retention rate in the hostile ischemic microenvironment, and promoting the secretion of important cytokines for reparative mechanisms. CONCLUSION Biomaterials have a relatively high potential for enhancing stem cell therapy. Nonetheless, there is a scarcity of evidence from human clinical studies for the efficacy of this bioengineered cell therapy, highlighting that it is still too early to draw a definitive conclusion on efficacy and safety for patient usage. Future in-depth clinical investigations are necessary to realize translation of this therapy into a more conscientious and judicious evidence-based therapy for clinical application.

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Section: Discussionmentioning

confidence: 99%

Biomaterial application strategies to enhance stem cell-based therapy for ischemic stroke

Satar¹,

Othman²,

Tan³

2022

World J Stem Cells

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“…Importantly, this encapsulation approach preserves cell viability and proliferation without adverse effects such as inflammatory reactions [ 255 , 256 ]. Representative compositions involve the coating of negatively charged islets [ 257 ] with positively charged polymers, such as cationic gelatin [ 256 , 258 , 259 ] and chitosan [ 260 ], followed by subsequent coating with anionic polymers (e.g., alginate, heparin, hyaluronic acid) [ 256 , 258 , 259 , 261 ]. This approach minimizes the required transplant volume for cell transplantation by employing nanometer-sized ultrathin layers (<50 nm) of polymer coating [ 250 ].…”

Section: Cell Encapsulation Techniquesmentioning

confidence: 99%

“…In addition, the nanoscale isolation layer decreases the diffusion distance between the encapsulated islets and the host environment, thereby enhancing the efficacy of the transplantation process [ 262 ]. The thickness of the polymer shell [ 261 ] and the incorporation of diverse functionalities, including drug cargos, through multiple layers [ 262 , 263 ] can be easily controlled in LbL self-assembly processes.…”

Section: Cell Encapsulation Techniquesmentioning

confidence: 99%

“…Thus far, most applications of LbL self-assembly focused on the encapsulation of pancreatic islets [ 253 , 263 , 264 , 265 , 266 , 267 , 268 , 269 ] and neural stem cells [ 259 , 261 ]. Combination of countercharged gelatin and alginate stands as the most popular composition for the encapsulation of mammalian cells as these polymers display opposite charges under the same pH conditions [ 270 ], as well as high cell compatibility.…”

Section: Cell Encapsulation Techniquesmentioning

confidence: 99%

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Recent Advances in Alginate-Based Hydrogels for Cell Transplantation Applications

Kavand,

Noverraz,

Gerber-Lemaire

2024

Pharmaceutics

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With its exceptional biocompatibility, alginate emerged as a highly promising biomaterial for a large range of applications in regenerative medicine. Whether in the form of microparticles, injectable hydrogels, rigid scaffolds, or bioinks, alginate provides a versatile platform for encapsulating cells and fostering an optimal environment to enhance cell viability. This review aims to highlight recent studies utilizing alginate in diverse formulations for cell transplantation, offering insights into its efficacy in treating various diseases and injuries within the field of regenerative medicine.

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“…The procedures for SEM were performed as previously described (22). Briefly, 131 I-PLGA-rhTSH nanoparticles were loaded onto coverslips.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Poly (lactic-co-glycolic acid)-encapsulated iodine-131 nanoparticles fabricated with rhTSH induce apoptosis and immobilization of thyroid cancer cells

Fan

Xiong²,

Wang³

et al. 2023

Front. Oncol.

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BackgroundAggressive thyroid carcinoma (ATC) usually loses radioiodine avidity to iodine-131 (131I) due to the downregulation of sodium/iodide symporter (NIS). The expression of thyroid stimulating hormone receptor (TSHR) is more persistent than NIS and the administration of recombinant human thyroid stimulating hormone (rhTSH) promotes de novo NIS synthesis. Hence, exploring methods integrating 131I with rhTSH might be a feasible therapeutic strategy for selective delivery of 131I into thyroid cancer to fortify the effect of radioiodine ablation.MethodsThe 131I, poly (lactic-co-glycolic acid) (PLGA) and rhTSH were used to synthesize of the 131I-PLGA-rhTSH nanoparticles. The characteristics of the 131I-PLGA-rhTSH nanoparticles was determined using a light microscopy, scanning electron microscopy (SEM), autoradiography and immunofluorescence (IF) staining. The diameter of the 131I-PLGA-rhTSH nanoparticles was measured with a Mastersizer 3000, and the encapsulation efficiency (EF) of 131I in 131I-PLGA-rhTSH nanoparticles and the radioactivity of a single nanoparticle were determined. Then, the mouse tumor xenograft model was established, and the biodistribution and effect of 131I-PLGA-rhTSH nanoparticles on apoptosis of thyroid cance cells were investigated in vivo. Thereafter, the role of 131I-PLGA-rhTSH nanoparticles in cell viability using cell counting kit-8 and lactate dehydrogenase (LDH) release assays. Subsequently, the underlying mechanism of 131I-PLGA-rhTSH nanoparticles in reducing cell viability was assessed using immunostaining, boyden invasion assays and phalloidin staining.ResultsOur results showed that the method of developing nanoparticles-encapsulated 131I using poly (lactic-co-glycolic acid) (PLGA) and modified with rhTSH (131I-PLGA-rhTSH), was a feasible avenue for the integration of 131I and rhTSH. Meanwhile, the encapsulation efficiency (EF) of 131I-PLGA-rhTSH nanoparticles was approximately 60%, and the radioactivity of a single nanoparticle was about 1.1×10-2 Bq. Meanwhile, the 131I-PLGA-rhTSH nanoparticles were selectively delivered into, gradually enriched and slowly downregulated in xenograft tumor after the administration of 131I-PLGA-rhTSH nanoparticles through tail vein in mouse tumor xenograft model. Thereafter, the tumor weight was significantly reduced after the administration of 131I-PLGA-rhTSH nanoparticles. Subsequently, the application of 131I-PLGA-rhTSH nanoparticles facilitated apoptosis and attenuated immobilization via inhibiting F-actin assembling of FTC-133 cells.ConclusionThe present study develops a suitable approach integrating 131I and rhTSH, and this strategy is a feasible regimen enhancing the effect of radioiodine ablation for the treatment of thyroid cancer.

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Transplantation of layer-by-layer assembled neural stem cells tethered with vascular endothelial growth factor reservoir promotes neurogenesis and angiogenesis after ischemic stroke in mice

Cited by 7 publications

References 44 publications

Biomaterial application strategies to enhance stem cell-based therapy for ischemic stroke

Biomaterial application strategies to enhance stem cell-based therapy for ischemic stroke

Recent Advances in Alginate-Based Hydrogels for Cell Transplantation Applications

Poly (lactic-co-glycolic acid)-encapsulated iodine-131 nanoparticles fabricated with rhTSH induce apoptosis and immobilization of thyroid cancer cells

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