Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats

Tsai, Perng-Jy; Wang, Hwai Shi; Shyr, Yi Ming; Weng, Zen Chung; Tai, Ling Chen; Shyu, Jia Fwu; Chen, Tien Hua

doi:10.1186/1423-0127-19-47

Cited by 70 publications

(58 citation statements)

References 45 publications

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“…Work from other laboratories suggests HUC-MSCs transplanted into brain striatum are viable for 4 months without immunological suppression 26. Moreover, transplantation with HUC-MSCs in rat spinal cord and liver also promotes the regeneration of corticospinal fibers and controls the type 1 diabetes, respectively 27. Together these studies suggest that HUC-MSCs may be an important source for allogeneic transplantation therapy in the future.…”

Section: Discussionmentioning

confidence: 98%

Enhancement of mouse germ cell-associated genes expression by injection of human umbilical cord mesenchymal stem cells into the testis of chemical-induced azoospermic mice

et al. 2014

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Various methods are currently under investigation to preserve fertility in males treated with high-dose chemotherapy and radiation for malignant and nonmalignant disorders. Human umbilical cord mesenchymal stem cells (HUC-MSCs), which possess potent immunosuppressive function and secrete various cytokines and growth factors, have the potential clinical applications. As a potential alternative, we investigate whether injection of HUC-MSCs into the interstitial compartment of the testes to promote spermatogenic regeneration efficiently. HUC-MSCs were isolated from different sources of umbilical cords and injected into the interstitial space of one testis from 10 busulfan-treated mice (saline and HEK293 cells injections were performed in a separate set of mice) and the other testis remained uninjected. Three weeks after MSCs injection, Relative quantitative reverse transcription polymerase chain reaction was used to identify the expression of 10 of germ cell associated, which are all related to meiosis, demonstrated higher levels of spermatogenic gene expression (2–8 fold) in HUC-MSCs injected testes compared to the contralateral uninjected testes (five mice). Protein levels for germ cell-specific genes, miwi, vasa and synaptonemal complex protein (Scp3) were also higher in MSC-treated testes compared to injected controls 3 weeks after treatment. However, no different expression was detected in saline water and HEK293 cells injection control group. We have demonstrated HUC-MSCs could affect mouse germ cell-specific genes expression. The results also provide a possibility that the transplanted HUC-MSCs may promote the recovery of spermatogenesis. This study provides further evidence for preclinical therapeutic effects of HUC-MSCs, and explores a new approach to the treatment of azoospermia.

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Section: Discussionmentioning

confidence: 98%

Enhancement of mouse germ cell-associated genes expression by injection of human umbilical cord mesenchymal stem cells into the testis of chemical-induced azoospermic mice

et al. 2014

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“…Furthermore, after the transplantation of differentiated cells into the portal vein of the diabetic rats using a Port-A catheter, blood sugar levels were shown to decrease. Insulin-producing cells containing human C-peptide and human nuclei were observed in the liver (48). In the study by Trivedi et al (49), five patients with type 1 diabetes mellitus were successfully infused with unfractionated cultured bone marrow plus human adipose-tissue-derived, insulin-making MSCs.…”

Section: C B Amentioning

confidence: 99%

Therapeutic efficacy of umbilical cord-derived mesenchymal stem cells in patients with type 2 diabetes

Guan

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. Type 2 diabetes (T2D) is characterized by progressive and inexorable β-cell dysfunction, leading to insulin deficiency. Novel strategies to preserve the remaining β-cells and restore β-cell function for the treatment of diabetes are urgently required. Mesenchymal stem cells (MSCs) have been exploited in a variety of clinical trials aimed at reducing the burden of immune-mediated disease. The aim of the present clinical trial was to assess the safety and efficacy of umbilical cord-derived MSC (UCMSC) transplantation for patients with T2D. The safety and efficacy of UCMSC application were evaluated in six patients with T2D during a minimum of a 24-month follow-up period. Following transplantation, the levels of fasting C-peptide, the peak value and the area under the C-peptide release curve increased significantly within one month and remained high during the follow-up period (P<0.05). Three of the six patients became insulin free for varying lengths of time between 25 and 43 months, while the additional three patients continued to require insulin injections, although with a reduced insulin requirement. Fasting plasma glucose and 2-h postprandial blood glucose levels were relatively stable in all the patients following transplantation. There was no immediate or delayed toxicity associated with the cell administration within the follow-up period. Therefore, the results indicated that transplantation of allogeneic UCMSCs may be an approach to improve islet function in patients with T2D. There were no safety issues observed during infusion and the long-term monitoring period.

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“…HUCMSCs can differentiate into insulinproducing cells in vitro (68,72). Using a portal vein injection, HUCMSC-differentiated islet cells can alleviate hyperglycemia in diabetic rats (68) and mice (72).…”

Section: Endodermmentioning

confidence: 99%

Human Umbilical Cord Mesenchymal Stem Cells: A New Era for Stem Cell Therapy

et al. 2015

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The human umbilical cord is a promising source of mesenchymal stem cells (HUCMSCs). Unlike bone marrow stem cells, HUCMSCs have a painless collection procedure and faster self-renewal properties. Different derivation protocols may provide different amounts and populations of stem cells. Stem cell populations have also been reported in other compartments of the umbilical cord, such as the cord lining, perivascular tissue, and Wharton's jelly. HUCMSCs are noncontroversial sources compared to embryonic stem cells. They can differentiate into the three germ layers that promote tissue repair and modulate immune responses and anticancer properties. Thus, they are attractive autologous or allogenic agents for the treatment of malignant and nonmalignant solid and soft cancers. HUCMCs also can be the feeder layer for embryonic stem cells or other pluripotent stem cells. Regarding their therapeutic value, storage banking system and protocols should be established immediately. This review critically evaluates their therapeutic value, challenges, and future directions for their clinical applications.

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Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats

Cited by 70 publications

References 45 publications

Enhancement of mouse germ cell-associated genes expression by injection of human umbilical cord mesenchymal stem cells into the testis of chemical-induced azoospermic mice

Enhancement of mouse germ cell-associated genes expression by injection of human umbilical cord mesenchymal stem cells into the testis of chemical-induced azoospermic mice

Therapeutic efficacy of umbilical cord-derived mesenchymal stem cells in patients with type 2 diabetes

Human Umbilical Cord Mesenchymal Stem Cells: A New Era for Stem Cell Therapy

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