Transplantation of Immortalized Human Fetal Hepatocytes Prevents Acute Liver Failure in 90% Hepatectomized Mice

Chen, Y.; Li, J.; Liu, X.; Zhao, Wenran; Wang, Y.; Wang, X.

doi:10.1016/j.transproceed.2010.01.061

Cited by 19 publications

(13 citation statements)

References 21 publications

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“…Human foetal hepatocytes are also considered as a potent alternative human hepatocyte source (29,30). A major source of human foetal hepatocyte is the liver of a therapeutically aborted foetus, and such abortions usually occur at the early stages of pregnancy.…”

Section: A B Cmentioning

confidence: 99%

Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells

et al. 2015

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Adult hepatocytes are polarised with their apical and basolateral membranes separated from neighbouring cells by tight junction proteins. Although efficient differentiation of pluripotent stem cells to hepatocytes has been achieved, the formation of proper polarisation in these cells has not been thoroughly investigated. In the present study, human embryonic stem cells (hESCs) and human mesenchymal stem cells (hMSCs) were differentiated to hepatocyte-like cells and the derived hepatocytes were characterised for mature hepatocyte markers. The secretion of hepatic proteins, expression of hepatic genes and the functional hepatic polarisation of stem cell-derived hepatocytes, foetal hepatocytes and the HepG2 hepatic cell line were evaluated and the different lines were compared. The results indicate that hESC-derived hepatocytes are phenotypically more robust and functionally more efficient compared with the hMSC-derived hepatocytes, suggesting their suitability for toxicity studies.

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Section: A B Cmentioning

confidence: 99%

Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells

et al. 2015

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“…Pareja et al [87] performed hepatocyte transplantation in acute-on-chronic liver failure patients with encouraging results, including improvement of hyperammonemia and degree of encephalopathy [87]. Similarly, transplantation of immortalized human fetal hepatocytes significantly improved survival in mice after 90% hepatectomy [88]. Coencapsulation of hepatocytes with bone marrow-derived MSCs not only augments engraftment [89] and lengthens hepatocyte viability, but also enhances hepatocyte-specific functions both in vitro and in vivo [90].…”

Section: Potential Applications Of Stem Cells In Liver Diseasesmentioning

confidence: 99%

Stem Cell Therapies for Treatment of Liver Disease

et al. 2016

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Cell therapy is an emerging form of treatment for several liver diseases, but is limited by the availability of donor livers. Stem cells hold promise as an alternative to the use of primary hepatocytes. We performed an exhaustive review of the literature, with a focus on the latest studies involving the use of stem cells for the treatment of liver disease. Stem cells can be harvested from a number of sources, or can be generated from somatic cells to create induced pluripotent stem cells (iPSCs). Different cell lines have been used experimentally to support liver function and treat inherited metabolic disorders, acute liver failure, cirrhosis, liver cancer, and small-for-size liver transplantations. Cell-based therapeutics may involve gene therapy, cell transplantation, bioartificial liver devices, or bioengineered organs. Research in this field is still very active. Stem cell therapy may, in the future, be used as a bridge to either liver transplantation or endogenous liver regeneration, but efficient differentiation and production protocols must be developed and safety must be demonstrated before it can be applied to clinical practice.

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“…Even though fetal liver transplantation studies are showing promising results , their availability, the ethical concerns, possible tumorigenicity and their incomplete differentiation limits their use in clinical application .…”

Section: Cell Sources For Liver Tissue Engineeringmentioning

confidence: 99%

“…Functional comparison of fetal hepatocytes with mature hepatocytes in AMC-BAL shows that fetal hepatocytes are not functionally mature enough to employ in clinical treatment regimes (125). Even though fetal liver transplantation studies are showing promising results (126,127), their availability, the ethical concerns, possible tumorigenicity and their incomplete differentiation limits their use in clinical application (128).…”

Section: Human Fetal Hepatocytesmentioning

confidence: 99%

Liver tissue engineering and cell sources: issues and challenges

Palakkan

Hay

et al. 2013

Liver International

110

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Liver diseases are of major concern as they now account for millions of deaths annually. As a result of the increased incidence of liver disease, many patients die on the transplant waiting list, before a donor organ becomes available. To meet the huge demand for donor liver, alternative approaches using liver tissue engineering principles are being actively pursued. Even though adult hepatocytes, the primary cells of the liver are most preferred for tissue engineering of liver, their limited availability, isolation from diseased organs, lack of in vitro propagation and deterioration of function acts as a major drawback to their use. Various approaches have been taken to prevent the functional deterioration of hepatocytes including the provision of an adequate extracellular matrix and co-culture with non-parenchymal cells of liver. Great progress has also been made to differentiate human stem cells to hepatocytes and to use them for liver tissue engineering applications. This review provides an overview of recent challenges, issues and cell sources with regard to liver tissue engineering.

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Transplantation of Immortalized Human Fetal Hepatocytes Prevents Acute Liver Failure in 90% Hepatectomized Mice

Cited by 19 publications

References 21 publications

Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells

Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells

Stem Cell Therapies for Treatment of Liver Disease

Liver tissue engineering and cell sources: issues and challenges

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