Transplantation of IL-10-transfected endothelial progenitor cells improves retinal vascular repair via suppressing inflammation in diabetic rats

Wang, Ying; Fan, Lingling; Meng, Xiangda; Jiang, Feng; Chen, Qingzhong; Zhang, Zhuhong; Yan, Hua

doi:10.1007/s00417-016-3427-6

Cited by 21 publications

(11 citation statements)

References 30 publications

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“…In this study, GK treatment inhibited the expression of p-NF-κB/p65 and upregulated the Nrf2 pathway, indicating potential complicated crosstalk between NF-κB and Nrf2, which may contribute to the improvement in the inflammatory microenvironment of the brain and promote neuroprotection and nerve regeneration. In this study, the inhibition of NF-κB and TNF-α in OGD astrocytes treated with GK might be related to the increased expression of IL-10 ( 41 – 43 ). Additionally, a series of studies have demonstrated that the activation and nuclear translocation of Nrf2 and the expression of various antioxidant enzymes can be mediated via the PI3K/Akt pathway ( 44 , 45 ).…”

Section: Discussionmentioning

confidence: 64%

Comparing the role of Ginkgolide B and Ginkgolide K on cultured astrocytes exposed to oxygen‑glucose deprivation

Cao²,

Zhao

et al. 2018

Mol Med Report

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Ginkgolide B (GB) and ginkgolide K (GK) are two main active monomers of ginkgolides that present a unique group of diterpenes found naturally in the leaves of the Ginkgo biloba tree. Astrocytes are the most abundant cell type within the central nervous system (CNS) and serve essential roles in maintaining healthy brain function. The present study compared the biological effects of GB and GK on astrocytes exposed to oxygen-glucose deprivation (OGD). The results demonstrated that GB and GK exhibit many different actions. The level of the platelet-activating factor (PAF) was elevated on astrocytes exposed to OGD, and inhibited by GB and GK treatment. Although GB and GK inhibited the expression of p-NF-κB/p65, GK exerted stronger anti-inflammatory and antioxidant effects on astrocytes exposed to OGD than GB by inhibiting interleukin (IL)-6 and tumor necrosis factor-α, and inducing IL-10 and the nuclear factor-erythroid 2-related factor 2/HO-1 signaling pathway. When compared with GB treatment, GK treatment maintained high levels of phosphoinositide 3-kinase/phosphorylated-protein kinase B expression, and induced a marked upregulation of Wnt family member 1 and brain derived neurotrophic factor, indicating that GK, as a natural plant compound, may have more attractive prospects for clinical application in the treatment of neurological disorders than GB.

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Section: Discussionmentioning

confidence: 64%

Comparing the role of Ginkgolide B and Ginkgolide K on cultured astrocytes exposed to oxygen‑glucose deprivation

Cao²,

Zhao

et al. 2018

Mol Med Report

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“…EPC are regarded as a heterogeneous group of cells which can differentiate into endothelial cells directly or induce angiogenesis via paracrine action to restore impaired vasculature [183]. The functions of human EPC in ischemic retinal diseases have been evaluated in animal models of DR, ischemia/reperfusion and oxygeninduced retinopathy [184][185][186]. Transplantation of IL-10-transfected EPC from the rat bone marrow led to significant improvement of retinal structure and reduced retinal vascular permeability through inhibiting inflammation in diabetic rats [184].…”

Section: Cell-based Therapeutic Strategiesmentioning

confidence: 99%

Neurovascular unit in diabetic retinopathy: pathophysiological roles and potential therapeutical targets

Shen¹,

Lo²,

Mi³

et al. 2021

Eye and Vis

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Diabetic retinopathy (DR), one of the common complications of diabetes, is the leading cause of visual loss in working-age individuals in many industrialized countries. It has been traditionally regarded as a purely microvascular disease in the retina. However, an increasing number of studies have shown that DR is a complex neurovascular disorder that affects not only vascular structure but also neural tissue of the retina. Deterioration of neural retina could precede microvascular abnormalities in the DR, leading to microvascular changes. Furthermore, disruption of interactions among neurons, vascular cells, glia and local immune cells, which collectively form the neurovascular unit, is considered to be associated with the progression of DR early on in the disease. Therefore, it makes sense to develop new therapeutic strategies to prevent or reverse retinal neurodegeneration, neuroinflammation and impaired cell-cell interactions of the neurovascular unit in early stage DR. Here, we present current perspectives on the pathophysiology of DR as a neurovascular disease, especially at the early stage. Potential novel treatments for preventing or reversing neurovascular injuries in DR are discussed as well.

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“…These studies indicate that EPCs may protect organ injury through the attenuation of the activation of NF-kB induced by the inflammation and oxidative stress response. These data also indicate that the anti-inflammation of EPCs may be mainly due to the inhibition of EPCs on NF-kB 22,[36][37][38] . Interestingly, in contrast to pro-inflammatory factors, the EPC transplantation considerably upregulated anti-inflammatory factor IL-10 in both the BALF and serum in the VE group.…”

Section: Discussionmentioning

confidence: 64%

Endothelial Progenitor Cells Attenuate Ventilator-Induced Lung Injury with Large-Volume Ventilation

Geng

Wang

et al. 2019

Cell Transplant

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Ventilator-induced lung injury (VILI) is a common complication that results from treatment with mechanical ventilation (MV) in acute respiratory distress syndrome (ARDS) patients. The present study investigated the effect of endothelial progenitor cell (EPC) transplantation on VILI. Wistar rats were divided into three groups (n ¼ 8): sham (S), VILI model (V) induced by tidal volume ventilation (17 mL/kg), and VILI plus EPC transplantation (VE) groups. The lung PaO 2 /FiO 2 ratio, pulmonary wet-todry (W/D) weight ratio, number of neutrophils, total protein, neutrophil elastase level, and inflammatory cytokines in bronchoalveolar lavage fluid (BALF) and serum were examined. Furthermore, the histological and apoptotic analysis, and lung tissue protein expression analysis of Bax, Bcl-2, cleaved caspase-3, matrix metalloproteinase (MMP)-9, total nuclear factor kappa B (total-NF-kB), phosphorylated NF-kB (phospho-NF-kB) and myosin light chain (MLC) were performed. The ventilation-induced decrease in PaO 2 /FiO 2 ratio, and the increase in W/D ratio and total protein concentration were prevented by the EPC transplantation. The EPC transplantation (VE group) significantly attenuated the VILI-induced increased expression of tumor necrosis factor (TNF)-a, interleukin (IL)-1b, IL-8, MMP-9, phospho-NF-kB and MLC, neutrophil elastase levels and neutrophil counts in BALF. In addition, the anti-inflammatory factor IL-10 increased in the VE group. Furthermore, pulmonary histological injury and apoptosis (TUNEL-positive cells, increase in Bax and cleaved caspase-3) were considerably diminished by the EPC transplantation. The EPC transplantation ameliorated the VILI. The mechanism may be primarily through the improvement of epithelial permeability, inhibition of local and systemic inflammation, and reduction in apoptosis.

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Transplantation of IL-10-transfected endothelial progenitor cells improves retinal vascular repair via suppressing inflammation in diabetic rats

Cited by 21 publications

References 30 publications

Comparing the role of Ginkgolide B and Ginkgolide K on cultured astrocytes exposed to oxygen‑glucose deprivation

Comparing the role of Ginkgolide B and Ginkgolide K on cultured astrocytes exposed to oxygen‑glucose deprivation

Neurovascular unit in diabetic retinopathy: pathophysiological roles and potential therapeutical targets

Endothelial Progenitor Cells Attenuate Ventilator-Induced Lung Injury with Large-Volume Ventilation

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