Transplantation of human umbilical cord mesenchymal stem cells to treat premature ovarian failure

Abstract

As one of the problems and diseases for women before 40 years, premature ovarian failure (POF) could be characterized by amenorrhea, low estrogen levels, infertility, high gonadotropin levels, and lack of mature follicles. Causes of the disease involve some genetic disorders, autoimmunity diseases, and environmental factors. Various approaches have been employed to treat POF, however with limited success. Today, stem cells are used to treat POF, since they have the potential to self-repair and regenerate, and are effective in treating ovarian failure and infertility. As mesenchymal stem cell (MSC) could simultaneously activate several mechanisms, many researchers consider MSC transplantation to be the best and most effective approach in cell therapy. A good source for mesenchymal stem cells is human umbilical cord (HUCMSC). Animal models with cyclophosphamide are required for stem cell treatment and performance of HUCMSC transplantation. Stem cell therapy could indicate the levels of ovarian markers and follicle-stimulating hormone receptor. It also increases ovarian weight, plasma E2 levels, and the amount of standard follicles. Herein, the causes of POF, effective treatment strategies, and the effect of HUCMSC transplantation for the treatment of premature ovarian failure are reviewed. Many studies have been conducted in this field, and the results have shown that stem cell treatment is an effective approach to treat infertility.

Human Umbilical Cord Mesenchymal Stem Cells Improve Premature Ovarian Failure through Cell Apoptosis of miR-100-5p/NOX4/NLRP3

“…In this study, HUCMSCs promoted cell growth, increased the number of EdU cell and cell metastasis, and reduced caspase-3/9 activity levels in the in vitro model. Shareghi-Oskoue et al showed that HUCMSCs could treat premature ovarian failure [ 21 ]. These data indicated that HUCMSCs promoted cell recovery to improve premature ovarian failure.…”

Artificial Ovary for Young Female Breast Cancer Patients

“…Although it cannot directly differentiate to the oocyte, transplantation MSCs can secrete cytokines, signal, and growth factors to promote stem cells such as ESCs, iPSCs, and OSCs in artificial ovary differentiate into oocytes ( 92 ). MSCs can also simultaneously support nutrition and immune regulation for the ovary ( 93 ). It was reported that transplantation of MSCs can provide nearly 109 cytokines in the ovary to help recover follicles in POF patients ( 94 ).…”

Human Umbilical Cord Mesenchymal Stem Cells Efficiently Improve Neurobehavioral Status and Alleviate Brain Injury in Hypoxia/Ischemia-Induced Cerebral Palsy Rat Model Via Down-Regulating the Nogoa/Ngr/Rho Pathway

“…The adult Sprague-Dawley(SD) rats were purchesed from Hunan SJA Laboratory Animal Co., Ltd in China for reproduction rat pups. The rats were fed and watered freely in the SPF animal laboratory for 12 h day/night cycle, temperature (23)(24)(25)℃ and humidity (45-60%). A total of 80 rat pups were borned by adult SD rats and used for CP model.…”

“…The HUCMSCs from passage numbers 0-5 were characterized with ow cytometry (Becton, Dickinson and Company, USA). The cells were added with rabbit polyclonal antibodies conclude CD73, CD44 ,CD90 ,CD105 ,CD34,CD45,HLA-DR (Proteintech Inc,USA) ( 1:100 dilution) to detect the surface antigens of HUCMSCs [23].…”