Transplantation of human fetal brain cells into ischemic lesions of adult gerbil hippocampus

Abstract

This work demonstrates that xenotransplanted fetal human brain cells are able to survive in an ischemic lesion in a rodent model. These data might be useful for future neural transplantation studies of treatments for cerebrovascular ischemia in humans.

Proliferation, migration, and differentiation of human neural stem/progenitor cells after transplantation into a rat model of traumatic brain injury

“…Our data agree with those of previous studies demonstrating the integration of transplanted glioma or teratoma cells or embryonal tissue into the CNS of naive and injured animals. 2,35,39,45 The nontransformed cells used in this study are available for large-scale production and possibly clinical applications. The primary requirement of understanding posttransplantation behavior of human cells can be partially fulfilled by xenografting.…”

Ethanol Affects Differentiation‐Related Pathways and Suppresses Wnt Signaling Protein Expression in Human Neural Stem Cells

“…Isolated NSC were stained for CD133 and nestin expression as previously described (Barami et al., 2001; Vangipuram et al., 2008). Briefly, the cells were incubated with PE or FITC conjugated primary antibodies (anti‐CD133 [Miltenyi Biotechnologies], or anti‐nestin [R&D systems]), in the dark for 20 minutes at room temperature and fixed with Coulter fixative.…”

Ethanol Alters Cell Fate of Fetal Human Brain‐Derived Stem and Progenitor Cells

“…Isolated NSC were stained for CD133 and nestin, and GCP for A2B5, and NCP for PSA‐NCAM as previously described (Barami et al., 2001; Vangipuram et al., 2008). Briefly, the cells were incubated with PE or flourescein isothiocyanate (FITC) conjugated primary antibodies [anti‐CD133, anti‐A2B5, anti‐PSA‐NCAM (Miltenyi Biotechnologies), or anti‐nestin (R&D systems)], in the dark for 20 minutes at room temperature and fixed with Coulter fixative.…”