Transplantation of ex vivo expanded cord blood

Shpall, E.J.; McNiece, I.K.; Lima, Marcos de; McMannis, John D.; Robinson, Scott; Peled, Tony; Champlin, R. E.

doi:10.1016/j.bbmt.2004.06.028

Cited by 103 publications

(134 citation statements)

References 1 publication

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“…These can be used clinically to treat children with malignant diseases or inborn errors (Laughlin et al 2001;Barker and Wagner 2003;Gluckman et al 2004). Ex vivo expansion can be performed using whole UCB units prior to transplantation (Pecora et al 2000;Shpall et al 2002). It is hoped and required that the UCB cells can be expanded in a short period of time in order to fit the needs of clinical transplantation.…”

Section: Introductionmentioning

confidence: 99%

Effective expansion of umbilical cord blood hematopoietic stem/progenitor cells by regulation of microencapsulated osteoblasts under hypoxic condition

et al. 2009

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The expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood (UCB) with the support of microencapsulated osteoblasts under hypoxia environment was investigated. The expansion of HSPCs was evaluated through the total number of UCB mononuclear cells (MNCs) produced, their repopulating potential with the colony-forming unit assay (CFU-Cs) and CD34(+) phenotypic analysis with flow cytometry. At the end of 7 days of culture, the UCB-MNCs, CFU-Cs and CD34(+) cells had achieved 18.7 +/- 1.6, 11.6 +/- 0.9 and 23.4 +/- 2-fold expansions, respectively, in the test groups. These were significantly different from those in control groups. Microencapsulated osteoblasts under hypoxia conditions had therefore a significant effect on the expansion potential of HSPCs in vitro.

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Section: Introductionmentioning

confidence: 99%

Effective expansion of umbilical cord blood hematopoietic stem/progenitor cells by regulation of microencapsulated osteoblasts under hypoxic condition

et al. 2009

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“…Hence, ex vivo expanded CB HSC has been coupled with unmanipulated CB for clinical transplantations (4,(39)(40)(41). This co-transplantation method serves two purposes.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Human Bone Marrow Stroma-Derived Heparan Sulfate on the Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells

et al. 2010

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“…It has been shown that the growth potential of HSC derived from CB is significantly elevated when compared to HSC from adult sources [11,12]. It seems that HSC can be expanded in vitro [13]. However, it remains unclear whether early progenitors are lost during this in vitro treatment, and thus the long-term re-population capacities would be negatively affected.…”

Section: Advantages and Disadvantages Of Hsc From Cbmentioning

confidence: 99%

“…However, it is not clear yet whether primitive progenitors are lost during the in vitro procedures, and thus long-term engraftment properties would be negatively affected. It seems that the engraftment kinetics are not increased by in vitro expansion [13]. Whether the optimization of cytokines and the addition of MSC increase the number of CD34+lin− cells is currently under investigation [43,44].…”

Section: Outcome Of Umbilical Cord Hsct In Adultsmentioning

confidence: 99%

Cord blood for allogeneic and autologous banking

Troeger

Hösli

Holzgreve

2007

Eur Clinics Obstet Gynaecol

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Allogeneic transplantations of haematopoietic stem cells derived from cord blood have become a clinical routine. Applications are successful in malignant and nonmalignant diseases, with high engraftment capacities and, compared to bone marrow stem cell transplantations, less strict human leucocyte antigen-match criteria. A major concern in using haematopoietic stem cells from cord blood is the relatively low number of stem cells that can be retrieved; however, recent research focuses on using several cord blood transplants as tandem transplants or increasing the number of progenitors by in vitro expansion strategies. In contrast to these running applications in allogeneic stem cell transplantation, autologous stem cell transplantations so far are very rare, and future indications, such as in regenerative medicine, are still experimental.

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Transplantation of ex vivo expanded cord blood

Cited by 103 publications

References 1 publication

Effective expansion of umbilical cord blood hematopoietic stem/progenitor cells by regulation of microencapsulated osteoblasts under hypoxic condition

Effective expansion of umbilical cord blood hematopoietic stem/progenitor cells by regulation of microencapsulated osteoblasts under hypoxic condition

The Effect of Human Bone Marrow Stroma-Derived Heparan Sulfate on the Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells

Cord blood for allogeneic and autologous banking

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