Transplantation of bone marrow mesenchymal stem cells pretreated with valproic acid in rats with an acute spinal cord injury

Chen, Lei; Cui, Xiaoyan; Wu, Zhourui; Jia, Lingyun; Yu, Yan; Zhou, Qing; Hu, Xiao; Xu, Wei; Luo, Dandan; Liu, Jie; Xiao, Junjie; Qiao, Yan; Cheng, Liming

doi:10.5582/bst.8.111

Cited by 22 publications

(13 citation statements)

References 31 publications

(44 reference statements)

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“…T8-T12 spinal cords in longitudinal sections were dissected from 6 rats in each group and one in every ten sections derived from each animal was used to determine the area of cystic cavity in each group (Chen et al 2014).…”

Section: Histological Analysismentioning

confidence: 99%

Adipose-Derived Stem Cells Expressing the Neurogenin-2 Promote Functional Recovery After Spinal Cord Injury in Rat

Tang

Zhu

et al. 2015

Cell Mol Neurobiol

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Neurogenin2 (Ngn2) is a proneural gene that directs neuronal differentiation of progenitor cells during development. This study aimed to investigate whether the use of adipose-derived stem cells (ADSCs) over-expressing the Ngn2 transgene (Ngn2-ADSCs) could display the characteristics of neurogenic cells and improve functional recovery in an experimental rat model of SCI. ADSCs from rats were cultured and purified in vitro, followed by genetically modified with the Ngn2 gene. Forty-eight adult female Sprague-Dawley rats were randomly assigned to three groups: the control, ADSCs, and Ngn2-ADSCs groups. The hind-limb motor function of all rats was recorded using the Basso, Beattie, and Bresnahan locomotor rating scale for 8 weeks. Moreover, hematoxylineosin staining and immunohistochemistry were also performed. After neural induction, positive expression rate of NeuN in Ngn2-ADSCs group was upon 90 %. Following transplantation, a great number of ADSCs was found around the center of the injury spinal cord at 1 and 4 weeks, which improved retention of tissue at the lesion site. Ngn2-ADSCs differentiated into neurons, indicated by the expression of neuronal markers, NeuN and Tuj1. Additionally, transplantation of Ngn2-ADSCs upregulated the trophic factors (brain-derived neurotrophic factor and vascular endothelial growth factor), and inhibited the glial scar formation, which was indicated by immunohistochemistry with glial fibrillary acidic protein. Finally, Ngn2-ADSCs-treated animals showed the highest functional recovery among the three groups. These findings suggest that transplantation of Ngn2-overexpressed ADSCs promote the functional recovery from SCI, and improve the local microenvironment of injured cord in a more efficient way than that with ADSCs alone.

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Section: Histological Analysismentioning

confidence: 99%

Adipose-Derived Stem Cells Expressing the Neurogenin-2 Promote Functional Recovery After Spinal Cord Injury in Rat

Tang

Zhu

et al. 2015

Cell Mol Neurobiol

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“…To date, BMDC transplantation used as a therapeutic intervention in clinical phase I/II trials nearly eight yr. Experiments were firstly carried out in animal models, and most studies showed safe and effective in improving functional outcome without severely complications . Subsequently, meta‐analysis and system reviews confirmed that it was an efficient and safe way in treating SCI in animal models .…”

mentioning

confidence: 99%

Efficacy and safety of bone marrow‐derived cell transplantation for spinal cord injury: a systematic review and meta‐analysis of clinical trials

Zhong

Deng

et al. 2015

Clinical Transplantation

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“…Phosphatidylinositol 3-kinase and extracellular signal-regulated kinase 1/2 in bone marrow mesenchymal stem cells have been found to modulate the phosphorylation of microtubule-associated protein 1B via a cross-signaling network and have affected the migratory efficiency of bone marrow mesenchymal stem cells towards injured spinal cord [140]. Administration of valproic acid potentiates the therapeutic effect of mesenchymal stem cell therapy [141]. Interleukin-8 enhances the angiogenic potential of human bone marrow mesenchymal stem cells by increasing vascular endothelial growth factor production [142].…”

Section: Measures To Increase the Efficiency Of Bone Marrow Mesenchymmentioning

confidence: 99%

Prerequisites for Mesenchymal Stem Cell Transplantation in Spinal Cord Injury

Amr¹

2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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We have aimed at distinguishing obligatory prerequisites for mesenchymal stem cell transplantation in spinal cord injury from those prerequisites which are unnecessary or are prerequisites that have to be further investigated. Obligatory prerequisites include the following. First, the site of injury is extensively gliotic, constituting an unsuitable medium for stem cell transplantation. It has to be dissolved by neurolyzing agents, chondroitinase ABC as an example. Second, stem cells need a suitable biomaterial scaffold for their proper integration. Third, the biomaterial scaffold necessitates a tissue filler harboring stem cells, other cells and neurotrophic factors in a combinatorial approach. Fourth, the efficiency of mesenchymal stem cells themselves has to be increased (by reducing oxidative stress-induced apoptosis, by hypoxic preconditioning, by modulating the extracellular matrix and by other measures). Prerequisites that have to be further investigated include the ideal source, mode, quantity, time point and number of injections of mesenchymal stem cells; which growth factors and cells to be used in the combinatorial approach; transforming mesenchymal stem cells into motor neuron-like cells or Schwann cells; increasing the homing effect of stem cells and how to establish a continuous drug and cell delivery system.

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Transplantation of bone marrow mesenchymal stem cells pretreated with valproic acid in rats with an acute spinal cord injury

Cited by 22 publications

References 31 publications

Adipose-Derived Stem Cells Expressing the Neurogenin-2 Promote Functional Recovery After Spinal Cord Injury in Rat

Adipose-Derived Stem Cells Expressing the Neurogenin-2 Promote Functional Recovery After Spinal Cord Injury in Rat

Efficacy and safety of bone marrow‐derived cell transplantation for spinal cord injury: a systematic review and meta‐analysis of clinical trials

Prerequisites for Mesenchymal Stem Cell Transplantation in Spinal Cord Injury

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