Transplantation of bone-marrow-derived mesenchymal and epidermal stem cells contribute to wound healing with different regenerative features

Abstract

A new concept for wound therapy is the initiation of the regeneration of epidermal and dermal layers with appendages for skin function recovery. Bone-marrow-derived mesenchymal and epidermal stem cells (BMSCs and SSCs) are hypothesized to be able to home toward or to be transplanted to wound sites for skin repair and regeneration, but this awaits confirmation by further experimental and clinical evidence. In this study, the influence of the transplantation of BMSCs and SSCs with porous gelatin-β-tricalcium phosphate sponge as scaffolds on wound re-epithelization, collagen synthesis, skin tensile strength recovery, and skin appendage regeneration has been investigated. The transplantation of BMSCs or SSCs significantly accelerates wound re-epithelization, stimulates dermal collagen synthesis, and exhibits the trend to enhance the tensile strength recovery of skin. Furthermore, regenerative features of BMSCs and SSCs have been identified in activating blood vessel and hair follicle formation, respectively. These results not only provide experimental evidence for the application of BMSCs and SSCs as promising therapeutics for clinical wound treatment, but also display their characteristics in activating distinct skin appendage regeneration, which might have novel applications in skin tissue engineering.

Adipose-Derived Stem Cells Promote Peripheral Nerve Regeneration In Vivo without Differentiation into Schwann-Like Lineage

“…The gelatin hydrogel tubes were made from chemically cross-linked acidic gelatin as described by Peng et al and Yamamoto et al 21,22 The gelatin hydrogel was dispensed in a mold around a 20-gauge needle and dehydrated into tube shape overnight ( Fig. 1, above, left).…”

Notch1 Signaling Regulates Wound Healing via Changing the Characteristics of Epidermal Stem Cells

“…Activated ESCs migrate to the wound site for the replenishment of lost cells to aid in the re-epithelialization and repair of the damaged epithelium [5]. ESCs have also been shown to stimulate dermal collagen synthesis and enhance the tensile strength recovery of skin [6]. Under the correct guidance of signals, ESC differentiation contributes to wound repair.…”

Combined effect of ligament stem cells and umbilical-cord-blood-derived CD34+ cells on ligament healing

“…Umbilicalcord-blood-derived CD34+ cells have been reported to improve tissue recovery after injury by stimulating angiogenesis (Chen et al 2014;Rosenkranz et al 2012). An enhanced effect of combining various cell types on tissue regeneration has also been emphasized in recent studies (Williams et al 2013;Peng et al 2013;Bonaros et al 2006). Here, we evaluated the In the present study, we have demonstrated that umbilicalcord-blood-derived CD34+ cells and MCL-SCs can survive after being transplanted into the MCL injury site.…”