Transplantation of allogenic anterior lens capsule to the subretinal space in pigs

Kiilgaard, Jens Folke; Wiencke, Anne Katrine; Scherfig, Erik; Prause, Jan Ulrik; Cour, Morten la

doi:10.1034/j.1600-0420.2002.800115.x

Cited by 38 publications

(37 citation statements)

References 18 publications

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“…Also BC is not biodegradable in the human body, which can be beneficial since substrates developed with degradable materials and biological tissues can be difficult to handle and may induce retinal degeneration due to material degradation. [30][31][32][33][34][35][36] In this work, we evaluated the ability of RPE cells to adhere and grow on BC-based substrates.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

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The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 μm of average thickness) and heat-dried BC substrates were surface-modified via acetylation and polysaccharide adsorption, using chitosan and carboxymethyl cellulose. All substrates were characterized according to their surface chemistry, wettability, energy, topography, and also regarding their permeability, dimensional stability, mechanical properties, and endotoxin content. Then, their ability to promote RPE cell adhesion and proliferation in vitro was assessed. All surface-modified BC substrates presented similar permeation coefficients with solutes of up to 300 kDa. Acetylation of BC decreased it's swelling and the amount of endotoxins. Surface modification of BC greatly enhanced the adhesion and proliferation of RPE cells. All samples showed similar stress-strain behavior; BC and acetylated BC showed the highest elastic modulus, but the latter exhibited a slightly smaller tensile strength and elongation at break as compared to pristine BC. Although similar proliferation rates were observed among the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation. 2 ABSTRACT: The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 µm of average thickness) and heatdried BC substrates were surface modified via acetylation and polysaccharide adsorption using chitosan and carboxymethyl cellulose. The BC substrates were characterized according to surface chemistry, wettability, energy, topography, permeability, dimensional stability, mechanical properties and level of endotoxins present. Then, the ability to promote RPE cell adhesion and proliferation in vitro was assessed. BC substrates were porous and permeable to solutes up to 300 kDa. The acetylation decreased substrate swelling and the amount of endotoxins present. Surface modification greatly enhanced the adhesion and proliferation of RPE cells in BC. Although similar proliferation rates were observed between the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation.

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Section: Introductionmentioning

confidence: 99%

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

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“…Also, the pig eye and retina share many similarities with those of the human and are even more similar than those of other large non-primate mammals (Beauchemin 1974;Ruiz-Ederra et al 2005). Since the pig eye and retina closely resemble their human counterparts in size, number and distribution of rods and cones, shape, vasculature, and function, surgical and diagnostic equipment used in the clinic can readily be applied (Ghosh and Arnér 2002;Hendrickson and Hicks 2002;Kiilgaard et al 2002;Mahmoud et al 2003;Ruiz-Ederra et al 2005;Warfvinge et al 2005;Iandiev et al 2006;Lalonde et al 2006;Lassota et al 2006). The application of human ophthalmological diagnostics to the pig, such as optical coherence tomography, corneal topography imaging, and multifocal electroretinogram, allow for behavioral measurements (Janknecht et al 2001;Lalonde et al 2006;Voss-Kyhn et al 2007;Kyhn et al 2008Kyhn et al ,2009aNg et al 2008).…”

Section: Introductionmentioning

confidence: 99%

A Battery of Cell- and Structure-specific Markers for the Adult Porcine Retina

Johansson

Eftekhari

Warfvinge

2010

J Histochem Cytochem.

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S U M M A R Y The pig is becoming an increasingly used non-primate model in experimental studies of human retinal diseases and disorders. The anatomy, size, and vasculature of the porcine eye and retina closely resemble their human counterparts, which allows for application of standard instrumentation and diagnostics used in the clinic. Despite many reports that demonstrate immunohistochemistry as a useful method for exploring neuropathological changes in the mammalian central nervous system, including the pig, the porcine retina has been sparsely described. Hence, to facilitate further immunohistochemical analysis of the porcine retina, we report on the successful use of a battery of antibodies for staining of paraformaldehyde-fixed cryosectioned retina. The following antibodies were evaluated for neuronal cells and structures: recoverin (cones and rods), Rho4D2 (rods), transducin-g (cones), ROM-1 (photoreceptor outer segments), calbindin (horizontal cells), PKC-a (bipolar cells), parvalbumin (amacrine and displaced amacrine cells), and NeuN (ganglion cells and displaced amacrines). For detecting synaptic connections in fiber layers, we used an antibody against synaptobrevin. For detecting retinal pigment epithelium, we studied antibodies against cytokeratin and RPE65, respectively. The glial cell markers used were bFGF (Müller cells and displaced amacrine cells), GFAP (Müller cells and astrocytes), and vimentin (Müller cells). Each staining effect was evaluated with regard to its specificity, sensitivity, and reproducibility in the identification of individual cells, specific cell structures, and fiber layers, respectively. The markers parvalbumin and ROM-1 were tested here for the first time for the porcine retina. All antibodies tested resulted in specific staining of high quality. In conclusion, all immunohistochemical protocols presented here will be applicable in fixed, cryosectioned pig retina. (J Histochem Cytochem 58:377-389, 2010)

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“…This eye displayed a localized inflammation at the implantation site 7 days after PGS implantation, most probably caused by a wound-healing response elicited by rupture of Bruch's membrane. 18 No inflammation was found in the remaining eyes, which indicates that the PGS membrane was well tolerated in the subretinal space. The biodegradable nature of the membrane thus in most cases appears to allow gradual reattachment after detachment without concomitant inflammation.…”

Section: Discussionmentioning

confidence: 91%

Selective Removal of Photoreceptor CellsIn VivoUsing the Biodegradable Elastomer Poly(Glycerol Sebacate)

Ghosh

Neeley

Arnér

et al. 2011

Tissue Engineering Part A

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Retinal transplantation experiments have advanced considerably during recent years, but remaining diseased photoreceptor cells in the host retina physically obstruct the development of graft-host neuronal contacts that are required for vision. We here report selective removal of photoreceptors using the biodegradable elastomer poly(glycerol sebacate) (PGS). A 1Â3 mm PGS membrane was implanted in the subretinal space of normal rabbit eyes, and morphologic specimens were examined with hematoxylin and eosin staining and a panel of immunohistochemical markers. Seven days postoperatively, a patent separation of the neuroretina and retinal pigment epithelium was found as well as loss of several rows of photoreceptors in combination with massive terminal transferase-mediated dUTP nick-end labeling (TUNEL) staining for apoptosis in the outer nuclear layer. After 28 days, the neuroretina was reattached, the PGS membrane had degraded, and photoreceptors were absent in the implantation area. Activated Mü ller cells were found in the entire retina in 7-day specimens, and in the implantation area after 28 days. AII amacrine and rod bipolar cell morphology was not affected, except for disrupted dendritic branching, which was present in rod bipolar cells in 28-day specimens. We conclude that retinal detachment induced by the biodegradable PGS membrane creates a permissive environment in which graft-host neuronal connections may be facilitated in future retinal transplantation experiments.

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Transplantation of allogenic anterior lens capsule to the subretinal space in pigs

Cited by 38 publications

References 18 publications

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

A Battery of Cell- and Structure-specific Markers for the Adult Porcine Retina

Selective Removal of Photoreceptor CellsIn VivoUsing the Biodegradable Elastomer Poly(Glycerol Sebacate)

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