Transplantation of activated olfactory ensheathing cells by curcumin strengthens regeneration and recovery of function after spinal cord injury in rats

Guo, Jianbin; Cao, Guihua; Yang, Guoqing; Wang, Yakang; Song, Wei; Xu, Yayong; Ma, Tao; Li, Rui; Zhang, Qian; Hao, Dingjun; Yang, Hao

doi:10.1016/j.jcyt.2020.03.002

Cited by 26 publications

(52 citation statements)

References 40 publications

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“…The stimulated cells secreted larger amounts of growth factors in vivo along with improved immunomodulation of the injury site. The same study also reported that the OECs stimulated with curcumin exhibited increased phosphatidylserine receptor expression (Guo et al, 2020), suggesting that increased phagocytic activity may have aided the overall therapeutic outcomes. Thus, stimulating glial phagocytosis may improve neural repair therapies.…”

Section: Stimulating Phagocytosis Of Peripheral Glia To Treat Neural Injuriesmentioning

confidence: 75%

“…In vitro injury assays (neurons co-cultured with OECs accompanied by neuronal debris addition), showed that increased OEC clearance of debris due to application of stimuli (LPS + curcumin Hao et al, 2017or TGF-β Li et al, 2017 promoted neuronal survival. A recent study in a rat spinal cord injury model reported that transplanted OECs that had been pre-treated with curcumin led to better functional recovery and axonal regrowth than transplantation with control OECs (Guo et al, 2020). The stimulated cells secreted larger amounts of growth factors in vivo along with improved immunomodulation of the injury site.…”

Section: Stimulating Phagocytosis Of Peripheral Glia To Treat Neural Injuriesmentioning

confidence: 99%

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Phagocytosis by Peripheral Glia: Importance for Nervous System Functions and Implications in Injury and Disease

Nazareth

John

Murtaza

et al. 2021

Front. Cell Dev. Biol.

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The central nervous system (CNS) has very limited capacity to regenerate after traumatic injury or disease. In contrast, the peripheral nervous system (PNS) has far greater capacity for regeneration. This difference can be partly attributed to variances in glial-mediated functions, such as axon guidance, structural support, secretion of growth factors and phagocytic activity. Due to their growth-promoting characteristic, transplantation of PNS glia has been trialed for neural repair. After peripheral nerve injuries, Schwann cells (SCs, the main PNS glia) phagocytose myelin debris and attract macrophages to the injury site to aid in debris clearance. One peripheral nerve, the olfactory nerve, is unique in that it continuously regenerates throughout life. The olfactory nerve glia, olfactory ensheathing cells (OECs), are the primary phagocytes within this nerve, continuously clearing axonal debris arising from the normal regeneration of the nerve and after injury. In contrast to SCs, OECs do not appear to attract macrophages. SCs and OECs also respond to and phagocytose bacteria, a function likely critical for tackling microbial invasion of the CNS via peripheral nerves. However, phagocytosis is not always effective; inflammation, aging and/or genetic factors may contribute to compromised phagocytic activity. Here, we highlight the diverse roles of SCs and OECs with the focus on their phagocytic activity under physiological and pathological conditions. We also explore why understanding the contribution of peripheral glia phagocytosis may provide us with translational strategies for achieving axonal regeneration of the injured nervous system and potentially for the treatment of certain neurological diseases.

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Section: Stimulating Phagocytosis Of Peripheral Glia To Treat Neural Injuriesmentioning

confidence: 75%

Section: Stimulating Phagocytosis Of Peripheral Glia To Treat Neural Injuriesmentioning

confidence: 99%

Phagocytosis by Peripheral Glia: Importance for Nervous System Functions and Implications in Injury and Disease

Nazareth

John

Murtaza

et al. 2021

Front. Cell Dev. Biol.

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“…In the process of filling the scaffold to promote the repair of the injury, the edge of the injury is surrounded by astrocyte scars, which hinders nerve regeneration at a later stage. Studies have shown that OEC can be fused with astrocytes to induce new axons through glial scars 9,23 . Therefore, we believe that OEC can cooperate with the scaffold on the injury site, and promote the growth of axon in the injury site at the later stage of the injury, and make the axon pass through the astrocyte scar, thereby improving the microenvironment of axon regeneration at the injury site.…”

Section: Discussionmentioning

confidence: 95%

Olfactory ensheathing cells seeded decellularized scaffold promotes axonal regeneration in spinal cord injury rats

et al. 2020

J Biomedical Materials Res

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Spinal cord decellularized (DC) scaffolds can promote axonal regeneration and restore hindlimb motor function of spinal cord defect rats. However, scarring caused by damage to the astrocytes at the margin of injury can hinder axon regeneration. Olfactory ensheathing cells (OECs) integrate and migrate with astrocytes at the site of spinal cord injury, providing a bridge for axons to penetrate the scars and grow into lesion cores. The purpose of this study was to evaluate whether DC scaffolds carrying OECs could better promote axon growth. For these studies, DC scaffolds were cocultured with primary extracted and purified OECs. Immunofluorescence and electron microscopy were used for verification of cells adhere and growth on the scaffold. Scaffolds with OECs were transplanted into rat spinal cord defects to evaluate axon regeneration and functional recovery of hind limbs. Basso, Beattie, and Bresnahan (BBB) scoring was used to assess motor function recovery, and glial fibrillary acidic protein (GFAP) and NF200‐stained tissue sections were used to evaluate axonal regeneration and astrological scar distribution. Our results indicated that spinal cord DC scaffolds have good histocompatibility and spatial structure, and can promote the proliferation of adherent OECs. In animal experiments, scaffolds carrying OECs have better axon regeneration promoting protein expression than the SCI model, and improve the proliferation and distribution of astrocytes at the site of injury. These results proved that the spinal cord DC scaffold with OECs can promote axon regeneration at the site of injury, providing a new basis for clinical application.

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“…In an animal study of traumatic spinal cord injury in Sprague Dawley rats, transplantation of olfactory ensheathing cells treated with curcumin promoted neural regeneration and functional recovery. Curcumin led to the production of anti-inflammatory and neurotrophic factors and the reduction in pro-inflammatory cytokines [83]. Finally, in human astrocytes and the white matter injury model of SCI, curcumin inhibited hydrogen peroxide (H 2 O 2 )-induced oxidative stress, reduced hypoxia-induced expression of HIF-1, GFAP, and NF-H proteins, and inhibited apoptosis [84].…”

Section: Spinal Cord Injurymentioning

confidence: 99%

Curcumin Formulations and Trials: What’s New in Neurological Diseases

et al. 2020

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Curcumin’s pharmacological properties and its possible benefits for neurological diseases and dementia have been much debated. In vitro experiments show that curcumin modulates several key physiological pathways of importance for neurology. However, in vivo studies have not always matched expectations. Thus, improved formulations of curcumin are emerging as powerful tools in overcoming the bioavailability and stability limitations of curcumin. New studies in animal models and recent double-blinded, placebo-controlled clinical trials using some of these new formulations are finally beginning to show that curcumin could be used for the treatment of cognitive decline. Ultimately, this work could ease the burden caused by a group of diseases that are becoming a global emergency because of the unprecedented growth in the number of people aged 65 and over worldwide. In this review, we discuss curcumin’s main mechanisms of action and also data from in vivo experiments on the effects of curcumin on cognitive decline.

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Transplantation of activated olfactory ensheathing cells by curcumin strengthens regeneration and recovery of function after spinal cord injury in rats

Cited by 26 publications

References 40 publications

Phagocytosis by Peripheral Glia: Importance for Nervous System Functions and Implications in Injury and Disease

Phagocytosis by Peripheral Glia: Importance for Nervous System Functions and Implications in Injury and Disease

Olfactory ensheathing cells seeded decellularized scaffold promotes axonal regeneration in spinal cord injury rats

Curcumin Formulations and Trials: What’s New in Neurological Diseases

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