Transplantation of acellular dermis and keratinocytes cultured on porous biodegradable microcarriers into full-thickness skin injuries on athymic rats

Seland, Håvard; Gustafson, Carl-Johan; Johnson, Hans; Junker, Johan P.E.; Kratz, Gunnar

doi:10.1016/j.burns.2010.03.014

Cited by 25 publications

(9 citation statements)

References 30 publications

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“…Generating epidermal sheets is time-consuming and costly, and the resulting products have a short shelf life (<24 hours). However, autologous epidermal grafts can be life-saving and have enjoyed numerous innovations at various stages of production, including improvements to cell culture techniques, differentiation techniques, and support/ scaffolding assembly (40,41). Today, autologous epidermal grafts capable of covering the entire surface area of the body can be generated from a 3-cm 2 biopsy (42).…”

Section: Bioengineered Skin Equivalentsmentioning

confidence: 99%

Advances in skin grafting and treatment of cutaneous wounds

Sun

Siprashvili

Khavari

2014

Science

677

484

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The ability of the skin to repair itself after injury is vital to human survival and is disrupted in a spectrum of disorders. The process of cutaneous wound healing is complex, requiring a coordinated response by immune cells, hematopoietic cells, and resident cells of the skin. We review the classic paradigms of wound healing and evaluate how recent discoveries have enriched our understanding of this process. We evaluate current and experimental approaches to treating cutaneous wounds, with an emphasis on cell-based therapies and skin transplantation.

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Section: Bioengineered Skin Equivalentsmentioning

confidence: 99%

Advances in skin grafting and treatment of cutaneous wounds

Sun

Siprashvili

Khavari

2014

Science

677

484

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“…Efforts have been made to create modified models, where contraction is retarded to more closely mimic the physiology of human wound healing, for example, by splinting 7 or by suturing the wound edges to the underlying tissue to prevent contraction. 8 Morris et al 9 first described the rabbit ear excision model, which displays raised scars reminiscent of hypertrophic scars in humans, but does not heal by contraction. Others have since used other sites on the rabbit for evaluation of treatments for full-thickness skin defects, 10,11 where contraction does occur.…”

Section: Wound Contraction In Animal Modelsmentioning

confidence: 99%

Strategies Demonstrating Efficacy in Reducing Wound ContractionIn Vivo

Sharpe

Martin

2013

Advances in Wound Care

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Significance: Scarring continues to present a significant clinical problem. Wound contraction leads to scarring and is mediated by myofibroblasts and contractile forces across the wound bed. Contracture formation can have a significant impact on the quality of life of the patient, particularly where function and appearance are affected. Recent Advances: Novel tissue-engineered matrices, cell-based therapies, and medicinal therapeutics have shown significant reduction in wound contraction in in-vivo models, particularly at early time points. These have been accompanied in many cases by reduced numbers of myofibroblasts, and in some by increased angiogenesis and improved neodermal architecture. Critical Issues: There are no animal models that replicate all aspects of wound healing as seen in patients. Therefore, information obtained from in vivo studies should be assessed critically. Additional studies, in particular those that seek to elucidate the mechanisms by which novel therapies reduce contraction, are needed to gain sufficient confidence to move into clinical testing. Future Directions: The use of knockout mouse models in particular has generated significant advances in knowledge of the mechanisms behind myofibroblast conversion and other factors involved in generating tension across the wound. Medicinal therapeutics and tissue-engineering approaches that seek to disrupt/alter these pathways hold much promise for future development and translation to clinical practice. SCOPEFull-thickness wounds heal with scarring, due to the abnormal arrangement of de novo collagen fibres in the neodermis and contractile forces exerted on the wound environment and surrounding tissue. Scar formation can have a negative impact on the patient and continues to present a significant clinical challenge. In vivo models take into account the physiological response to injury and the complex interactions that occur during wound repair that cannot be replicated in vitro. This review summarizes recent in vivo studies that have demonstrated efficacy in reducing wound contraction in model organisms. TRANSLATIONAL RELEVANCEThe demonstration of efficacy in vivo of a potential therapy to reduce wound contraction provides strong evidence of potential clinical efficacy. Further investigation and investment can then be undertaken to translate the novel therapy to early-stage clinical studies. In some cases, the manipulation, through the application of novel wound therapies, of the complex cascade of events leading to wound repair may have

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“…In the recent past, the use of various nanoparticle based microspheres, specifically PLGA, and natural polymers such as collagen or gelatin microspheres, has been reported for developing dermal or skin regeneration scaffolds for the effective delivery of drugs such as antibiotics or growth factors [177,178,179,180,181,182,183]. The size of microspheres in the scaffolds can be adjusted for the controlled release of proteins or drugs [177].…”

Section: Scaffolding Approaches and Different Types Of Scaffolds Imentioning

confidence: 99%

Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review

Chaudhari

Vig

Baganizi

et al. 2016

IJMS

448

288

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Over centuries, the field of regenerative skin tissue engineering has had several advancements to facilitate faster wound healing and thereby restoration of skin. Skin tissue regeneration is mainly based on the use of suitable scaffold matrices. There are several scaffold types, such as porous, fibrous, microsphere, hydrogel, composite and acellular, etc., with discrete advantages and disadvantages. These scaffolds are either made up of highly biocompatible natural biomaterials, such as collagen, chitosan, etc., or synthetic materials, such as polycaprolactone (PCL), and poly-ethylene-glycol (PEG), etc. Composite scaffolds, which are a combination of natural or synthetic biomaterials, are highly biocompatible with improved tensile strength for effective skin tissue regeneration. Appropriate knowledge of the properties, advantages and disadvantages of various biomaterials and scaffolds will accelerate the production of suitable scaffolds for skin tissue regeneration applications. At the same time, emphasis on some of the leading challenges in the field of skin tissue engineering, such as cell interaction with scaffolds, faster cellular proliferation/differentiation, and vascularization of engineered tissues, is inevitable. In this review, we discuss various types of scaffolding approaches and biomaterials used in the field of skin tissue engineering and more importantly their future prospects in skin tissue regeneration efforts.

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Transplantation of acellular dermis and keratinocytes cultured on porous biodegradable microcarriers into full-thickness skin injuries on athymic rats

Cited by 25 publications

References 30 publications

Advances in skin grafting and treatment of cutaneous wounds

Advances in skin grafting and treatment of cutaneous wounds

Strategies Demonstrating Efficacy in Reducing Wound ContractionIn Vivo

Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review

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