Transplantation in Drosophila

Beadle, G. W.; Ephrussi, Boris

doi:10.1073/pnas.21.12.642

Cited by 49 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ovaries were dissected from females fattened for two days on wet yeast in Ephrussi Beadle Ringers (EBR) medium ( Beadle and Ephrussi, 1935 ). Ovaries were pulsed with 5-ethynyl-2-deoxyuridine (EdU) for 30 min, fixed in 4% formaldehyde and prepared for immunofluorescence (IF) as described ( Nordman et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Rif1 inhibits replication fork progression and controls DNA copy number in Drosophila

Munden

Rong

Sun

et al. 2018

eLife

View full text Add to dashboard Cite

Control of DNA copy number is essential to maintain genome stability and ensure proper cell and tissue function. In Drosophila polyploid cells, the SNF2-domain-containing SUUR protein inhibits replication fork progression within specific regions of the genome to promote DNA underreplication. While dissecting the function of SUUR’s SNF2 domain, we identified an interaction between SUUR and Rif1. Rif1 has many roles in DNA metabolism and regulates the replication timing program. We demonstrate that repression of DNA replication is dependent on Rif1. Rif1 localizes to active replication forks in a partially SUUR-dependent manner and directly regulates replication fork progression. Importantly, SUUR associates with replication forks in the absence of Rif1, indicating that Rif1 acts downstream of SUUR to inhibit fork progression. Our findings uncover an unrecognized function of the Rif1 protein as a regulator of replication fork progression.

show abstract

Section: Methodsmentioning

confidence: 99%

Rif1 inhibits replication fork progression and controls DNA copy number in Drosophila

Munden

Rong

Sun

et al. 2018

eLife

View full text Add to dashboard Cite

show abstract

“…Ovaries were dissected in Ephrussi Beadle Ringers (EBR) solution (Beadle and Ephrussi, 1935) or Grace's unsupplemented medium from females fattened for two days on wet yeast and pulse labeled with 50μM EdU for 30 minutes. Ovaries were prepared for antibody staining as indicated in (Royzman et al, 1999) with modifications detailed in the Supplemental Experimental Procedures.…”

Section: Methodsmentioning

confidence: 99%

DNA Copy-Number Control through Inhibition of Replication Fork Progression

et al. 2014

View full text Add to dashboard Cite

Summary Proper control of DNA replication is essential to ensure faithful transmission of genetic material and to prevent chromosomal aberrations that can drive cancer progression and developmental disorders. DNA replication is regulated primarily at the level of initiation and is under strict cell cycle regulation. Importantly, DNA replication is highly influenced by developmental cues. In Drosophila, specific regions of the genome are repressed for DNA replication during differentiation by the SNF2 domain-containing protein SUUR through an unknown mechanism. We demonstrate that SUUR is recruited to active replication forks and mediates repression of DNA replication by directly inhibiting replication fork progression instead of functioning as a replication fork barrier. Mass-spec identification of SUUR associated proteins identified the replicative helicase member CDC45 as a SUUR-associated protein, supporting a role for SUUR directly at replication forks. Our results reveal that control of eukaryotic DNA copy number can occur through inhibition of replication fork progression.

show abstract

“…Ovaries were dissected from females fattened for two days on wet yeast in Ephrussi Beadle 572 Ringers (EBR) medium (Beadle and Ephrussi, 1935). Ovaries were pulsed with 5-ethynyl-2-573 deoxyuridine (EdU) for 30 minutes, fixed in 4% formaldehyde and prepared for 574 immunofluorescence (IF) as described (Nordman et al, 2014).…”

Section: Cytological Analysis and Microscopy: 571mentioning

confidence: 99%

Rif1 inhibits replication fork progression and controls DNA copy number in Drosophila

Munden

Rong

Gangula

et al. 2018

Preprint

View full text Add to dashboard Cite

Control of DNA copy number is essential to maintain genome stability and ensure proper cell and tissue function. In Drosophila polyploid cells, the SNF2-domain-containing SUUR protein inhibits replication fork progression within specific regions of the genome to promote DNA underreplication. While dissecting the function of SUUR’s SNF2 domain, we identified a physical interaction between SUUR and Rif1. Rif1 has many roles in DNA metabolism and regulates the replication timing program. We demonstrate that repression of DNA replication is dependent on Rif1. Rif1 localizes to active replication forks in an SUUR-dependent manner and directly regulates replication fork progression. Importantly, SUUR associates with replication forks in the absence of Rif1, indicating that Rif1 acts downstream of SUUR to inhibit fork progression. Our findings uncover an unrecognized function of the Rif1 protein as a regulator of replication fork progression.

show abstract

Transplantation in Drosophila

Cited by 49 publications

References 0 publications

Rif1 inhibits replication fork progression and controls DNA copy number in Drosophila

Rif1 inhibits replication fork progression and controls DNA copy number in Drosophila

DNA Copy-Number Control through Inhibition of Replication Fork Progression

Rif1 inhibits replication fork progression and controls DNA copy number in Drosophila

Contact Info

Product

Resources

About