Transplantable Osteosarcomas With High Lung Metastatic Potential in Fischer 344 Rats

Abstract

Both spontaneous (SOS) and 4‐hydroxyaminoquinoline 1‐oxide (4‐HAQO)‐induced osteosarcomas (COS) could be serially transplanted in the subcutaneous back space of syngeneic F344 rats, the success rate becoming 100% within 5 passage generations. Transplanted tumors demonstrated rapid growth and displayed high potential for metastasis to the lung in later generations. Tbus, a 100% lung metastasis rate was observed for SOS after the 20th and for COS after the 14th generation. The histological features of the primary SOS and COS were retained during serial transfer. These model systems should be useful for investigation of the biology of this very important tumor type.

Inhibition of Spontaneous Rat Osteosarcoma Lung Metastasis by 3S‐[4‐(Nhydroxyamino)‐2R‐isobutylsuccinyl]amino‐1‐methoxy‐3,4‐dihydrocarbostyril, a Novel Matrix Metalloproteinase Inhibitor

“…Previously, we established transplantable osteosarcomas with rapid growth and high lung metastatic potential after serial transplantation into the subcutaneous back space of syngeneic rats. [2][3][4][5][6][7] They represent useful osteosarcoma models for testing novel anti-metastatic therapies.…”

Establishment and Utility of Transplantable Osteosarcomas in Fisher 344 Rats

“…Recently Mii et al 4 reported the establishment of transplantable cell lines of rat osteosarcoma producing osteoid and bone tissues. In this study I also succeeded in establishing subcutaneously transplantable osteosarcoma cell lines producing bone and osteoid tissue, and showing ALP activity.…”

Telomerase Activity Correlates with Growth of Transplantable Osteosarcomas in Rats Treated with cis‐Diammine Dichloroplatinum or the Angiogenesis Inhibitor AGM‐1470

“…Details regarding the methods of tumor production, handling and transplantations have been described previously. [20][21][22][23][24][25] All procedures were performed under aseptic conditions. Experimental protocol Lung metastatic nodules, approximately 3 mm in diameter, were transplanted into the subcutaneous back space of rats with the following basic protocol.…”

“…18) Osteosarcoma is the most prevalent and important malignant bone tumor of youth in man, having a poor prognosis with rapid growth and frequent distant metastasis, especially to the lung. 19) Previously, we established rat transplantable osteosarcomas with low and high metastatic potentials, [20][21][22][23][24][25] with increased expression of H-ras, 21) nm23, 21) c-fos and c-jun 22) and increased telomerase activity, 25) and we suggested that they might be useful as experimental tools to study the biological behavior of this type of malignancy. We also reported inhibitory effects of cis-diammine dichloroplatinum (CDDP), a frequently used agent for osteosarcomas treatment, [26][27][28] and O-(chloroace-tylcarbamoyl)fumagillol (AGM-1470), a well known antiangiogenic agent 29,30) considered to be a potent candidate for osteosarcoma treatment, using our osteosarcoma model.…”