Transphyletic conservation of nitric oxide synthase regulation in cephalochordates and tunicates

Caccavale, Filomena; Coppola, Ugo; Vassalli, Quirino Attilio; Vecchia, Claudia La; Palumbo, Anna; D’Aniello, Enrico; Locascio, Annamaria; Ristoratore, Filomena; D’Aniello, Salvatore

doi:10.1007/s00427-020-00668-3

Cited by 3 publications

(9 citation statements)

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“…By using in situ hybridization, immunochemistry, and biochemical methods to detect NOS expression and NO messenger diffusion territories, we have taken a snapshot of the Ciona larval NO pathway localization. In addition, three No s genomic fragments previously identified by Caccavale et al [ 32 ] were used to fully define the NOS localization pattern.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, phylogenetic and syntenic analyses have provided novel insights into the origin of NOS proteins, suggesting that evolution was the result of multiple gene and genome duplication events together with changes in protein architecture [ 1 ]. Whereas in mammals NO acts mainly as neurotransmitter, vasodilator, and immune response mediator, in marine invertebrates the gas is involved in a variety of biological processes, including stress response to environmental pollutants or toxins [ 5 , 6 , 7 , 8 , 9 , 10 ], defense [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ], neurotransmission [ 13 , 24 ], swimming [ 25 ], feeding [ 26 , 27 ], symbiosis [ 28 ], and fertilization and development [ 29 , 30 , 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, common mechanisms of NOS regulation in non-vertebrate chordates were disclosed [ 32 ]. Putative regulatory regions of Nos identified in Branchiostoma lanceolatum and C. robusta were shown to function as enhancers during Ciona development.…”

Section: Introductionmentioning

confidence: 99%

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Novel Insights on Nitric Oxide Synthase and NO Signaling in Ascidian Metamorphosis

Locascio

Vassalli

Castellano

et al. 2022

IJMS

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Nitric oxide (NO) is a pivotal signaling molecule involved in a wide range of physiological and pathological processes. We investigated NOS/NO localization patterns during the different stages of larval development in the ascidia Ciona robusta and evidenced a specific and temporally controlled pattern. NOS/NO expression starts in the most anterior sensory structures of the early larva and progressively moves towards the caudal portion as larval development and metamorphosis proceeds. We here highlight the pattern of NOS/NO expression in the central and peripheral nervous system of Ciona larvae which precisely follows the progression of neural signals of the central pattern generator necessary for the control of the movements of the larva towards the substrate. This highly dynamic localization profile perfectly matches with the central role played by NO from the first phase of settlement induction to the next control of swimming behavior, adhesion to substrate and progressive tissue resorption and reorganization of metamorphosis itself.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel Insights on Nitric Oxide Synthase and NO Signaling in Ascidian Metamorphosis

Locascio

Vassalli

Castellano

et al. 2022

IJMS

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“…Lancelet specimens were collected between 2012 and 2017 in five European localities (two from the Atlantic Ocean, two from the western Mediterranean Sea, and one from the centraleastern Mediterranean Sea) and including populations widely exploited for Evo-Devo studies [28,[52][53][54][55][56]. Noteworthy, specimens were also sampled from near the type localities of Limax lanceolatus Pallas, 1774 (Cornwall: see [20]) and Branchiostoma lubricum Costa, 1834 (Naples, Italy: see [47]), the only confirmed subjective junior synonym of B. lanceolatum.…”

Section: Samplingmentioning

confidence: 99%

“…Genomic DNA was extracted from adult lancelets as previously described [56]. Partial sequences of three mitochondrial genes widely used for taxonomic studies, namely cytochrome c oxidase subunit I (COX1), 12S ribosomal ribonucleic acid (12S rRNA), and 16S ribosomal ribonucleic acid (16S rRNA), were amplified from three specimens randomly selected from each sampling locality, using the following species-specific primers designed for this study using OligoEvaluator™: Cox1_forward 5'-GATTCATAATATGCGTGCTAGC-3' and Cox1_ reverse 5'-CGGCTCCTATAGACAAAACG-3'; 12S_forward 5'-GGGTTACTGATGAT ACATGC-3' and 12S_reverse 5'-CTACTATTGACTACACCCTG-3'; 16S_forward 5'-CGCCTGTTTAACAAAAACAT-3' and 16S_reverse 5'-CGGTCTGTACTCAGATCA CGTA-3'.…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

Molecular taxonomy confirms that the northeastern Atlantic and Mediterranean Sea harbor a single lancelet, Branchiostoma lanceolatum (Pallas, 1774) (Cephalochordata: Leptocardii: Branchiostomatidae)

et al. 2021

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Branchiostomatidae (lancelets or amphioxus) comprises about 30 species, several of which are well-established models in evolutionary development. Our zoological and ecological knowledge of the family is nonetheless limited. Despite evident differences can be found among known populations, the taxonomy of Branchiostoma lanceolatum (type species of the genus Branchiostoma) has never been investigated with modern methods through its range in the northeastern Atlantic and Mediterranean Sea. We address this via a multilocus molecular approach and comparing specimens collected from different European populations. Results obtained here confirm the presence of a single species inhabiting the range between the topotypical localities of B. lanceolatum (Atlantic Ocean) and of its junior synonym B. lubricum (Mediterranean Sea), without evincing geographical structure between populations. This suggests that environment most likely drives the characteristics observed in different geographic areas. The long larval phase and the slow mutation rate in lancelets may have played a key role in the evolutionary history of this iconic species.

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Nitric Oxide Function and Nitric Oxide Synthase Evolution in Aquatic Chordates

Locascio

Annona

Caccavale

et al. 2023

IJMS

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Nitric oxide (NO) is a key signaling molecule in almost all organisms and is active in a variety of physiological and pathological processes. Our understanding of the peculiarities and functions of this simple gas has increased considerably by extending studies to non-mammal vertebrates and invertebrates. In this review, we report the nitric oxide synthase (Nos) genes so far characterized in chordates and provide an extensive, detailed, and comparative analysis of the function of NO in the aquatic chordates tunicates, cephalochordates, teleost fishes, and amphibians. This comprehensive set of data adds new elements to our understanding of Nos evolution, from the single gene commonly found in invertebrates to the three genes present in vertebrates.

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Transphyletic conservation of nitric oxide synthase regulation in cephalochordates and tunicates

Cited by 3 publications

References 63 publications

Novel Insights on Nitric Oxide Synthase and NO Signaling in Ascidian Metamorphosis

Novel Insights on Nitric Oxide Synthase and NO Signaling in Ascidian Metamorphosis

Molecular taxonomy confirms that the northeastern Atlantic and Mediterranean Sea harbor a single lancelet, Branchiostoma lanceolatum (Pallas, 1774) (Cephalochordata: Leptocardii: Branchiostomatidae)

Nitric Oxide Function and Nitric Oxide Synthase Evolution in Aquatic Chordates

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