Transpecies heart valve transplant: advanced studies of a bioengineered xeno-autograft

Goldstein, Steven A.; Clarke, David R.; Walsh, Steven P; Black, Kirby S.; O’Brien, Mark F.

doi:10.1016/s0003-4975(00)01812-9

Cited by 110 publications

(68 citation statements)

References 18 publications

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“…It has become increasingly clear that the assumption that xenoantigens are likely to be cell-associated is not valid. Our findings further emphasize that treatment of unfixed xenogeneic tissues for tissue engineering applications must shift from a paradigm of "decellularization" to one of "antigen removal" [9][10][11][12][14][15][16][17][18][19][20][21][22][23][24][25][26]. Current or future antigen removal or antigen masking treatments will need to account for both cellular and extracellular matrix antigens.…”

Section: Discussionmentioning

confidence: 99%

“…Because tissues are unfixed in this application, it is necessary to remove graft antigenicity prior to implantation. Numerous physical and chemical treatments designed to decrease the immunogenicity of unfixed xenogeneic biomaterials have been investigated [9,11,12,[14][15][16][17][18][19][20][21][22][23][24][25][26]. These treatments have generally been characterized as tissue "decellularization", based on the assumption that antigens mediating an immune response to the graft would likely be cell-associated.…”

Section: Introductionmentioning

confidence: 99%

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Immunoproteomic identification of bovine pericardium xenoantigens

et al. 2008

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Bovine pericardium is an important biomaterial with current application in glutaraldehyde-fixed bioprosthetic heart valves and possible future application as an unfixed biological scaffold for tissue engineering. The importance of both humoral and cell-mediated rejection responses toward fixed and unfixed xenogeneic tissues has become increasingly apparent. However, the full scope and specific identities of bovine pericardium proteins that can elicit an immune response remain largely unknown. In this study, an immunoproteomic approach was used to survey bovine pericardium proteins for their ability to elicit a humoral immune response in rabbits. A two-stage protein extraction protocol was used to separate bovine pericardium proteins into water-and lipid-soluble fractions. Two-dimensional gel electrophoresis was performed to separate the proteins from each fraction. Western blots were generated from two-dimensional gels of both bovine pericardium protein fractions. These blots were probed with serum from rabbits immunized with bovine pericardium and a secondary antibody was used to assess for IgG positivity. Western blots were compared to duplicate two-dimensional gels and proteins in matched spots were identified by tandem mass spectrometry. Thirty-one putative protein antigens were identified, eight of which are known to be antigenic from previous studies. All of the putative antigens demonstrated progressive staining intensity with increasing days of post-exposure serum. Identified antigenic proteins represented a variety of functional and structural protein types, and included both cellular and matrix proteins. The results of this study have implications for the use of bovine pericardium as a biomaterial in bioprostheses and tissue engineering applications, as well as xenotransplantation in general.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunoproteomic identification of bovine pericardium xenoantigens

et al. 2008

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“…This has been recognized as the FDA-recommended animal model for testing new designs of mechanical valves, tissue-based bioprosthetic valves, cryopreserved homografts and biohybrid devices (6,20,(23)(24)(25)(26)(27)(28). Although not initially developed for tissue engineering, sheep implantation continues to be the animal model of choice for testing of acellular valve scaffolds (29)(30)(31)(32)(33)(34). The juvenile sheep model is accessible, reproducible, accelerates valve calcification and approximates the human anatomy.…”

Section: Acellular Scaffolds and Their Behavior In Vivomentioning

confidence: 99%

Pulmonary heart valve replacement using stabilized acellular xenogeneic scaffolds; effects of seeding with autologous stem cells

Harpa

Movileanu

Sierad³

et al. 2015

Revista Romana De Medicina De Laborator

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“…Several authors have developed decellularization techniques followed by in vitro reseeding with autologous cells (Cebotari et al, 2000;Steinhoff et al, 2000) or allowing ingrowth of host cells into the graft after transplantation (Wilson et al, 1995;Goldstein et al, 2000;Steinhoff et al, 2000;Dohmen et al, 2002). In the case of arteries, the acellular matrix is composed of insoluble collagen, elastin, and glycosaminoglycans from the original vessel.…”

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confidence: 99%

Angiogenic response induced by acellular aortic matrix in vivo

et al. 2004

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In this study, we investigated the angiogenic response induced by acellular aortic matrices implanted in vivo onto the chick embryo chorioallantoic membrane (CAM), a useful model for such investigation. Results showed that acellular matrices were able to induce a strong angiogenic response comparable to that of fibroblast growth factor 2 (FGF-2), a wellknown angiogenic cytokine. The angiogenic response was further increased when exogenous FGF-2 or transforming growth factor beta 1 (TGF-␤1) were added to the matrices and inhibited by the addition of an anti-FGF-2 or anti-TGF-␤1 antibodies. The response may be considered dependent on a direct angiogenic effect exerted by the matrices and in part also by the presence of FGF-2 and TGF-␤1 in the acellular matrices.

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Transpecies heart valve transplant: advanced studies of a bioengineered xeno-autograft

Cited by 110 publications

References 18 publications

Immunoproteomic identification of bovine pericardium xenoantigens

Immunoproteomic identification of bovine pericardium xenoantigens

Pulmonary heart valve replacement using stabilized acellular xenogeneic scaffolds; effects of seeding with autologous stem cells

Angiogenic response induced by acellular aortic matrix in vivo

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