Transparent, resilient human amniotic membrane laminates for corneal transplantation

Hariya, Takehiro; Tanaka, Yuji; Yokokura, Shunji; Nakazawa, Toru

doi:10.1016/j.biomaterials.2016.05.038

Cited by 21 publications

(35 citation statements)

References 42 publications

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“…The use of substrates not only provided mechanical support during the transplantation of ex vivo -engineered CEC sheets but also created a favourable microenvironment for cellular activity 6 . Perfusion bioreactors have been shown to enhance the access of cells to oxygen and nutrients as well as the homogeneity of neo-synthesized ECM in 3D scaffolds 69, 70 .…”

Section: Discussionmentioning

confidence: 99%

“…Tissue-engineered endothelial grafts that utilize biomaterials and biomimetic scaffolds and incorporate the patient’s own cells have become a prospective alternative to allografts in the treatment of endothelial defects 5 . Although several studies have investigated the use of amniotic membranes and silk fibroin membranes as corneal substitutes, there are still insufficient data to permit further clinical trials 3, 6, 7 . The challenge of producing CEC layers or cells for endothelial cell therapy by tissue engineering is the phenotypic instability of CECs.…”

Section: Introductionmentioning

confidence: 99%

“…Acellular scaffolds such as the amniotic membrane and corneal stroma transmit light and possess mechanical properties that may support CEC attachment for transplantation applications in vivo 6, 31 . However, there is currently no reliable, standardized decellularization protocol to remove immunoreactive material and at the same time maintain tissue properties such as corneal curvature 32, 33 .…”

Section: Introductionmentioning

confidence: 99%

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In vitro biomimetic platforms featuring a perfusion system and 3D spheroid culture promote the construction of tissue-engineered corneal endothelial layers

Han

Lei

et al. 2017

Sci Rep

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Corneal endothelial cells (CECs) are very important for the maintenance of corneal transparency. However, in vitro, CECs display limited proliferation and loss of phenotype via endothelial to mesenchymal transformation (EMT) and cellular senescence. In this study, we demonstrate that continuous supplementary nutrition using a perfusion culture bioreactor and three-dimensional (3D) spheroid culture can be used to improve CEC expansion in culture and to construct a tissue-engineered CEC layer. Compared with static culture, perfusion-derived CECs exhibited an increased proliferative ability as well as formed close cell-cell contact junctions and numerous surface microvilli. We also demonstrated that the CEC spheroid culture significantly down-regulated gene expression of the proliferation marker Ki67 and EMT-related markers Vimentin and α-SMA, whereas the gene expression level of the CEC marker ATP1A1 was significantly up-regulated. Furthermore, use of the perfusion system in conjunction with a spheroid culture on decellularized corneal scaffolds and collagen sheets promoted the generation of CEC monolayers as well as neo-synthesized ECM formation. This study also confirmed that a CEC spheroid culture on a curved collagen sheet with controlled physiological intraocular pressure could generate a CEC monolayer. Thus, our results show that the use of a perfusion system and 3D spheroid culture can promote CEC expansion and the construction of tissue-engineered corneal endothelial layers in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro biomimetic platforms featuring a perfusion system and 3D spheroid culture promote the construction of tissue-engineered corneal endothelial layers

Han

Lei

et al. 2017

Sci Rep

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“…The construct was dried a final time before crosslinking with the EDC/NHS. The drying and crosslinking also had the added benefit of increasing the transparency of the constructs . It was found that at least four layers were needed to significantly increase the strength and clear the constructs; however, eight layers were needed to achieve the strength necessary to suture the constructs in place for a corneal keratoplasty in rabbits .…”

Section: Naturally Derived Biomaterials Used For Corneal Tissue Enginmentioning

confidence: 99%

Natural Biomaterials for Corneal Tissue Engineering, Repair, and Regeneration

Palchesko

Carrasquilla

Feinberg

2018

Adv Healthcare Materials

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Corneal blindness is a major cause of vision loss, estimated to affect over 10 million people worldwide. Once impaired through clouding or shape change, the best treatment option for restoring vision is corneal transplantation using full or partial thickness cadaveric grafts. However, donor corneas are globally limited and face rejection and graft failure, similar to other transplanted organs. Thus, there is a need for viable alternatives to donor corneas in order to increase supply, reduce rejection, and to minimize variability in tissue quality. To address this, researchers have developed new materials and strategies to tissue engineer full or partial thickness cornea grafts in order to repair, regenerate, or replace the diseased cornea. This progress report first reviews the anatomy and physiology of the cornea to frame the biological requirements and discuss the injuries and diseases that necessitate the need fortransplantation, as well as the requirements for a suitable donor tissue alternative. This is followed by recent progress using naturally derived biomaterials including silk, collagen, amniotic membranes, and decellularized corneas. Finally, remaining challenges in the field as they relate to the biomaterials discussed are identified, and the future research directions that should result in further advances in restoring corneal vision are highlighted.

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“…Lv et al prepared transparent TiO 2 nanotube membranes via anodization. Hariya et al created transparent and resilient amniotic membrane laminates by a newly developed multistep process that included dehydration and chemical cross‐linking. To fabricate electrically conductive and transparent films, which can be used as electrodes in flexible electronic devices, Bak et al prepared nylon nanofibrous membranes via electrospinning, which included dipping the membrane into the prepared single‐wall carbon nanotube (SWCNT) solutions, impregnating the SWCNT‐adsorbed membranes with a neat epoxy resin, and curing the epoxy resin through hot‐pressing.…”

Section: Introductionmentioning

confidence: 99%

Preparation of transparent polyacrylonitrile reinforced polyurethane film and application as temperature monitor

Zhao

Wang

Zhang

et al. 2017

Polymer Engineering & Sci

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This study proposed a new and facile technique to prepare transparent nanofiber reinforced film and evaluated light transmittance and mechanical properties of the film and film application as the temperature monitor. The technique used electrospinning composite solution and heating precursor composite nanofibrous film to package nanofibrous network with the resin. The results showed that the film with at least 90% light transmittance was facilely prepared by the technique. While when the concentration of polyacrylonitrile in the electrospun solution was 12 wt%, heating temperature was 200°C and heating time was 10 min, transparent film with above 95% light transmittance was developed, and showed desirable mechanical properties with the stress of 32.5 Mpa, strain of 51% and Young's modulus of 180 Mpa. Based on the fact that the transparent film changed the color with the temperature, the film showed a promising application as temperature monitor. POLYM. ENG. SCI., 58:1905–1910, 2018. © 2017 Society of Plastics Engineers

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Transparent, resilient human amniotic membrane laminates for corneal transplantation

Cited by 21 publications

References 42 publications

In vitro biomimetic platforms featuring a perfusion system and 3D spheroid culture promote the construction of tissue-engineered corneal endothelial layers

In vitro biomimetic platforms featuring a perfusion system and 3D spheroid culture promote the construction of tissue-engineered corneal endothelial layers

Natural Biomaterials for Corneal Tissue Engineering, Repair, and Regeneration

Preparation of transparent polyacrylonitrile reinforced polyurethane film and application as temperature monitor

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