Transparenchymal Suture Fixation and Testicular Histology in a Prepubertal Rat Model

Effect of testis nondescent or orchidopexy on antisperm antibodies and testis histology in rats

“…Among orchidopexies investigated, DP seems to be the technique of choice, as previously suggested (15), producing neither histologic…”

Testicular tissue histology in rabbit testes after transparenchymal suturing with six different types of suture material

“…In contrast to the work by Dixon et al, we did not evaluate how suture caliber affects tissue reaction [7]. However, the 6-0 caliber of the suture materials we used is smaller than the sizes that have been used in previous studies.…”

“…These authors observed that polytetrafluoroethylene caused greater changes in the parenchyma than polypropylene; however, they found no significant differences among the polypropylene, polytetrafluoroethylene, and silk materials with respect to changes in the testicular parenchyma. Dixon et al [7] analyzed tissue changes after testis fixation with very fine suture materials (3-0 and 5-0 sizes of chromic catgut, polyglactin, silk, polypropylene, and polydioxanone) during orchiopexy in 120 immature Sprague-Dawley rats. They found that all these suture materials resulted in significant inflammation and tubular damage, even when the smallest caliber was used.…”

“…The number of giant cells was scored on a four-point scale: 0 = no giant cells, 1 = 1 giant cell, 2 = 2-4 giant cells, and 3 C 5 giant cells. The proportion of the area infiltrated by histiocytes and the severity of fibrosis were both scored on a four-tier scale, based on the grading system proposed by Dixon et al [7]. In this system, 0 = no abnormality; 1 = mild lesion affecting\25% of the area; 2 = moderate lesion affecting 25-75% of the area; and 3 = severe lesion affecting[75% of the area.…”

Testicular Biopsy during Orchidopexy Procedure: Does It Have an Adverse Effect on Fertility?

“…These changes are thought to be associated with changes in seminiferous epithelium that result from impaired integrity of seminiferous channels or disrupted local blood and/or lymphatic circulation [21,22] . Apart from the testical biopsy procedure in which a part of testis parencyhma is removed, Dixon et al [23] found that even the suture material passing through capsule used for fi xing during orchidopexy alone might cause an infl ammatory response and damage in tubular structure and reported that this may have an effect on reproductive capacity. In a cohort study, Swerdlow et al [24] reported that incidence of malignancy was higher in subjects who underwent biopsy during orchidopexy procedure.…”