Transovarial transmission of dengue 3 virus by Aedes aegypti

Joshi, Vaibhav; Singhi, Manju; Chaudhary, Ranjana

doi:10.1016/s0035-9203(96)90416-2

Cited by 32 publications

(16 citation statements)

References 5 publications

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“…Joshi and others 4 have reported a high rate of transovarial transmission of dengue-3 virus in mosquitoes collected from Jodhpur (Rajasthan State), India. Mourya and others 5 suggested that this could be due to the fact that these fieldcollected mosquitoes originated from eggs that had remained conditioned and quiescent in the field for a longer time.…”

Section: Discussionmentioning

confidence: 99%

“…aegypti. 4 In a recent study, Mourya and others 5 have shown that transovarially infected mosquitoes can orally transmit the virus. This study also suggested that when eggs obtained from infected females are hatched after several weeks of incubation at room temperature, the rate of vertical transmission increases.…”

Section: Introductionmentioning

confidence: 98%

“…3 There are many reports of transovarial transmission of dengue viruses. [3][4][5] However, in the Rajasthan State of India, it has been shown to be a common, natural phenomenon in Ae. aegypti.…”

Section: Introductionmentioning

confidence: 99%

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Persistence of dengue-3 virus through transovarial transmission passage in successive generations of Aedes aegypti mosquitoes.

Joshi¹,

Mourya²,

Sharma³

2002

The American Journal of Tropical Medicine and Hygiene

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171

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Abstract. Progeny of Aedes aegypti mosquitoes infected intrathoracically with dengue-3 virus was reared to subsequent generations. In each generation, blood-fed females were confined individually and the eggs obtained from the transovarially infected females were pooled. The seventh generation obtained from the infected parental mosquitoes showed that virus could persist in mosquitoes in successive generations through transovarial passage. The rate of vertical transmission initially increased in the few generations (F 1 -F 2 ), but in subsequent generations it was found to be steady. Parental mosquitoes inoculated with virus showed higher mortality than the diluent-inoculated controls. There was an increase in the larval duration of transovarially infected batches at the seventh generation when compared with uninfected control mosquitoes. The fecundity and fertility of the transovarially infected batches of mosquitoes was also affected when compared with the controls. This is the first report demonstrating persistence of dengue virus in the successive generations of mosquitoes infected through vertical transmission. These observations, which have great epidemiologic importance, suggest that vector mosquitoes may play an important role in the maintenance of virus in nature, and that mosquitoes may act as reservoirs of these viruses.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Persistence of dengue-3 virus through transovarial transmission passage in successive generations of Aedes aegypti mosquitoes.

Joshi¹,

Mourya²,

Sharma³

2002

The American Journal of Tropical Medicine and Hygiene

Self Cite

171

128

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“…aegypti females collected in the field and pooled 3-4 days after emergence from larval stages. 23 In addition to the age of the emerging mosquitoes, several other factors would have contributed to the high levels of infection recorded in nature. Most of the males caught on humans were caught while they attempted to mate with females in search of a blood meal.…”

Section: Discussionmentioning

confidence: 99%

Vertical transmission of the yellow fever virus by Aedes aegypti (Diptera, Culicidae): dynamics of infection in F1 adult progeny of orally infected females.

Diallo¹,

Thonnon²,

Fontenille³

2000

The American Journal of Tropical Medicine and Hygiene

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Abstract. Vertical transmission of yellow fever virus from orally infected females to their progeny was experimentally demonstrated in 2 Aedes aegypti colonies from the Dakar and Koungheul regions in Senegal. A total of 10,530 F 1 adult mosquito progeny were tested. The overall vertical transmission rate was 0.97%, with no significant difference between the Dakar and Koungheul colonies. The infection rates were significantly higher in females (1.15%) than in males (0.74%) in both colonies. The virus was not isolated from the progeny of the first oviposition cycle (OVC1). The true infection rates were 0.27% and 1.99%, respectively, for the OVC2 and OVC3 progeny in the Dakar colony, and 1.1% and 1.48%, respectively, for the OVC2 and OVC3 progeny in the Koungheul colony. The infection rates increased with extrinsic incubation in both male and female offspring of the 2 colonies, reaching 5.2% in 20-day-old OVC3 female progeny in the Dakar colony. The epidemiologic consequences of these results are discussed.In 1995, a yellow fever epidemic occurred in Koungheul, Senegal. 1 Entomologic surveys provided evidence of vertical transmission of the yellow fever virus by its mosquito vector, Aedes aegypti. The virus was isolated from wild males and recently emerged adults from larvae collected in the field. 2 Vertical transmission of yellow fever virus has already been proved experimentally in Ae. aegypti, 3,4 and 3 strains of this virus have been isolated from male Ae. furcifer-taylori mosquitoes. 5 The capacity of other mosquito species to vertically transmit yellow fever virus has also been shown experimentally in Ae. mascarensis and Haemagogus equinus. 6,7 The studies conducted in Koungheul on Aedes mosquitoes collected in the field had shown that the infection rate in recently emerged adults was lower than that in males caught on humans. This observation suggested that the viral titer in emerging adults infected by vertical transmission was low and hard to detect with conventional isolation methods, whereas the older mosquitoes had a higher titer. The objective of this study was to study the dynamics of viral infection in emerging adult mosquitoes vertically infected, using the same isolation methods as those used during the Koungheul epidemic. MATERIALS AND METHODSMosquitoes. Two geographic strains of Ae. aegypti from Dakar, and Koungheul, Senegal were used. These colonies were reared at 27 Ϯ 1ЊC and a relative humidity of 70-80% with a 12-hr photoperiod. Adult mosquitoes were regularly fed on guinea pig blood or 10% sugar and larval stages were provided with Tetra Baby Fish Food (TetraWerke, Melle, Germany).Virus. The viral strain used was ArD 114891. It was isolated from a pool of 10 Ae. aegypti females caught on humans in the village of Koung-Koung during the epidemic that occurred in Koungheul, Senegal in 1995. This strain was passed twice on AP61 cell lines (Ae. pseudoscutellaris) and 3 times on suckling mice. The viral stock that we used in our experiments was prepared with triturated, infected suckling mice bra...

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“…On the other hand, all immatures specimens were negative by RT-PCR and virus isolation. This may be due to the small numbers of specimens collected during this study (Klin & Klin 1983, Hull et al 1984, Joshi et al 1996.…”

Section: Discussionmentioning

confidence: 95%

Detection of dengue virus serotype 3 by reverse transcription-polymerase chain reaction in Aedes aegypti (Diptera, Culicidae) captured in Manaus, Amazonas

Pinheiro

Tadei

Barros

et al. 2005

Mem. Inst. Oswaldo Cruz

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Transovarial transmission of dengue 3 virus by Aedes aegypti

Cited by 32 publications

References 5 publications

Persistence of dengue-3 virus through transovarial transmission passage in successive generations of Aedes aegypti mosquitoes.

Persistence of dengue-3 virus through transovarial transmission passage in successive generations of Aedes aegypti mosquitoes.

Vertical transmission of the yellow fever virus by Aedes aegypti (Diptera, Culicidae): dynamics of infection in F1 adult progeny of orally infected females.

Detection of dengue virus serotype 3 by reverse transcription-polymerase chain reaction in Aedes aegypti (Diptera, Culicidae) captured in Manaus, Amazonas

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