Transmitting tissue in the pistil of tobacco: Light and electron microscopic observations

Bell, Jim; Hicks, G. S.

doi:10.1007/bf00389993

Cited by 85 publications

(53 citation statements)

References 19 publications

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“…The occurrence of aniline blue-positive material in the component tissue of the pistil at different times after first opening of the flower is shown in Table 4, in which nomenclature of pistil tissues follows that of Bell and Hicks (1976). All preparations without aniline blue (Fig.…”

Section: Resultsmentioning

confidence: 99%

Reduced pollen tube growth and the presence of callose in the pistil of the male floral stage of the avocado

Sedgley

1977

Scientia Horticulturae

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Sedgley, M., 1977. Reduced pollen tube growth and the presence of callose in the pistil of the male floral stage of the avocado. Scientia Hort., 7 : 27--36.Pollen tube growth was studied in the pistil of the male floral stage of the avocado. Selfpollination of the male stage occurred but pollen tubes did not reach the ovary. When female and male stages were hand pollinated, fewer pollen tubes were present in the pistil of the male than in the female stage. A tube reached the ovary in only 1 of 110 male stage pistils, as compared with all female stage pistils observed. Flowers pollinated in the female stage were retained on the plant for longer than flowers pollinated in the male stage.Aniline blue-positive and resorcin blue-positive material (callose) was not present in the pistil of flowers in the female stage but occurred in 46 % of the aniline blue-stained and 30 % of the resorcin blue-stained pistils in the male floral stage. By 42 h after first opening, callose was present in every pistil observed, in association with the cell walls of the stigmatic papillae, the transmitting tissue, the vascular tissue or the cortex and epidermis or a combination of these tissues. Callose occured earlier in pollinated than in unpollinated pistils, particularly in the transmitting tissue.It is suggested that reduced pollen tube growth in the pistil of the male floral stage of the avocado is associated with the presence of callose.

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Section: Resultsmentioning

confidence: 99%

Reduced pollen tube growth and the presence of callose in the pistil of the male floral stage of the avocado

Sedgley

1977

Scientia Horticulturae

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“…The transmitting tissue thus provides not NETTANCOURT et al 1973;CREST! et al 1976a;DUMAS et al 1978SASSEN 1974BELL and HICKS 1976BREDEMEIJER 1979 SEDGLEY andBUTTROSE 1978;SEDGLEY 1979VANDER et al 1966KONAR and LINSKENS 1966a, b;KROH 1967KROH , 1973SASSEN 1974;HERRERO and DICKINSON 1979CREST! et al 1978 CONSIDINE and tv .j:>.…”

Section: The Style-pathway For Pollen Tube Growthmentioning

confidence: 98%

Pollen-Pistil Interactions

Knox¹

1984

Cellular Interactions

187

134

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“…Secretory substances occupy the extracellular matrix of the transmitting tissue. These extracellular deposits are highly enriched in sugar-containing substances, including glycoproteins, although their exact chemical constitution remains to be determined (Bell and Hicks, 1976;Clarke et al, 1979;Knox, 1984;Cresti et al, 1986). Tubes that emerge from pollen grains on the stigmatic surface are guided toward the l To whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

Development and Pollination Regulated Accumulation and Glycosylation of a Stylar Transmitting Tissue-Specific Proline-Rich Protein.

Wang

Cheung

1993

Plant Cell

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The extracellular matrix of stylar transmitting tissues of many angiosperms is enriched in secretory material$ that are believed to be important for interactions with pollen tubes. We have previously characterized two related cDNAs (TTS-1 and TTS-2) for stylar transmitting tissue-specific proline-rich proteins (TTS proteins) from Nicotiana tabacum. We show here that TTS proteins are highly glycosylated proteins with apparent molecular mames ranging between 50 and 100 kD. Results from chemical and enzymatic deglycosylation suggest that TTS proteins have N-linked glycosyl groups, and the extensive glycosylation most probably has resulted from modifications at the proline residues. TTS proteins are localized to the intercellular regions between neighboring transmitting tissue cells, the space in which pollen tubes elongate as they migrate from the stigma toward the ovary. TTS mRNA and protein levels are regulated during pistil development and by pollination. The levels of TTS mRNAs and proteins increase with flower development and reach the maximal levels as flowers approach anthesis. These maximal levels are maintained in the styles for at least 3 to 4 days after pollination, during which time pollen tubes elongate and reach the ovary. Spatially, TTS mRNAs and proteins accumulate first in the stigmatic end of young styles, and their levels progressively increase toward the basal end as pistils mature. Pollination stimulates the levels of TTS mRNAs and proteins in hand-pollinated young styles, which normally accumulate relatively low levels of these TTS gene products. Pollination also qualitatively affects TTS mRNAs and proteins. In pollinated styles, TTS mRNAs are shorter than those in unpollinated styles and underglycosylated TTS protein species begin to accumulate. The elaborate regulatory mechanisms governing TTS mRNAs and proteins during development and by pollination strongly suggest that these proteins may play a functional role in the process of pollination.

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Transmitting tissue in the pistil of tobacco: Light and electron microscopic observations

Cited by 85 publications

References 19 publications

Reduced pollen tube growth and the presence of callose in the pistil of the male floral stage of the avocado

Reduced pollen tube growth and the presence of callose in the pistil of the male floral stage of the avocado

Pollen-Pistil Interactions

Development and Pollination Regulated Accumulation and Glycosylation of a Stylar Transmitting Tissue-Specific Proline-Rich Protein.

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