Transmitter content, origins and connections of axons in the spinal cord that possess the serotonin (5-hydroxytryptamine) 3 receptor

Conte, Deborah; Legg, Ewen D.; McCourt, Andrew C; Silajdžić, Edina; Nagy, Gergely; Maxwell, David

doi:10.1016/j.neuroscience.2005.02.013

Cited by 44 publications

(41 citation statements)

References 32 publications

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“…In addition, the finding that blockade of 5-HT 3 receptors did not affect the inhibitory effect of fluvoxamine on monosynaptic C-fiber-mediated EPSCs suggests distinct roles of 5-HT 3 receptors on A-fiber and C-fiber terminals in modulating nociceptive synaptic transmission. Moreover, intrinsic GABAergic neurons also express 5-HT 3 receptors (35,36), activation of which has been shown to increase the release of GABA (37). More recently, Xie et al have shown that the 5-HT 3 receptor agonist mCPBG increases both the amplitude and frequency of GABAergic and glycinergic sIPSCs in SG neurons of rat spinal cord slices (38).…”

Section: Discussionmentioning

confidence: 99%

Presynaptic Inhibitory Effects of Fluvoxamine, a Selective Serotonin Reuptake Inhibitor, on Nociceptive Excitatory Synaptic Transmission in Spinal Superficial Dorsal Horn Neurons of Adult Mice

et al. 2014

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Abstract. Fluvoxamine, a selective serotonin (5-HT) reuptake inhibitor, has been shown to exert analgesic effects in humans and laboratory animals. However, its effects on spinal nociceptive synaptic transmission have not been fully characterized. Here, whole-cell recordings were made from dorsal horn neurons in spinal slices with attached dorsal roots from adult mice, and the effects of fluvoxamine on monosynaptic A-fiber-and C-fiber-mediated excitatory postsynaptic currents (EPSCs) evoked in response to electrical stimulation of a dorsal root were studied. Fluvoxamine (10 -100 mM) concentration-dependently suppressed both monosynaptic A-fiberand C-fiber-mediated EPSCs, which were attenuated by the selective 5-HT 1A receptor antagonist WAY100635. In the presence of the selective 5-HT 3 receptor antagonist tropisetron, fluvoxamine hardly suppressed A-fiber-mediated EPSCs, whereas its inhibitory effect on C-fiber-mediated EPSCs was not affected. Although fluvoxamine increased the paired-pulse ratio of A-fibermediated EPSCs, it increased the frequency of spontaneous and miniature EPSCs (sEPSCs and mEPSCs). Since sEPSCs and mEPSCs appeared to arise largely from spinal interneurons, we then recorded strontium-evoked asynchronous events occurring after A-fiber stimulation, whose frequency was reduced by fluvoxamine. These results suggest that fluvoxamine reduces excitatory synaptic transmission from primary afferent fibers via presynaptic mechanisms involving 5-HT 1A and/or 5-HT 3 receptors, which may contribute to its analgesic effects.

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Section: Discussionmentioning

confidence: 99%

Presynaptic Inhibitory Effects of Fluvoxamine, a Selective Serotonin Reuptake Inhibitor, on Nociceptive Excitatory Synaptic Transmission in Spinal Superficial Dorsal Horn Neurons of Adult Mice

et al. 2014

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“…Anatomical studies indicated that 5HT-positive terminals in both superficial and deep laminae at the Vc/C 1–2 region and cervical dorsal horn (Pearson and Jennes, 1988, Li et al, 1997). Similarly, the density of 5HT3R was highest in superficial laminae of spinal dorsal horn and punctate staining in deeper laminae (Maxwell et al, 2003, Conte et al, 2005). Local dorsal microcircuitry also may have contributed to the apparent differential effects of OND on TMJ neurons in superficial and deep laminae.…”

Section: Discussionmentioning

confidence: 91%

Inhibition of temporomandibular joint input to medullary dorsal horn neurons by 5HT3 receptor antagonist in female rats

et al. 2015

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Repeated forced swim (FS) conditioning enhances nociceptive responses to temporomandibular joint (TMJ) stimulation in male and female rats. The basis for FS-induced TMJ hyperalgesia remains unclear. To test the hypothesis that serotonin 3 receptor (5HT3R) mechanisms contribute to enhanced TMJ nociception after FS, ovariectomized female rats were treated with estradiol and subjected to FS for three days. On day 4, rats were anesthetized with isoflurane and TMJ-responsive neurons were recorded from superficial and deep laminae at the trigeminal subnucleus caudalis/upper cervical (Vc/C1–2) region and electromyographic (EMG) activity was recorded from the masseter muscle. Only Vc/C1–2 neurons activated by intra-TMJ injections of ATP were included for further analysis. Although neurons in both superficial and deep laminae were activated by ATP, only neurons in deep laminae displayed enhanced responses after FS. Local application of the 5HT3R antagonist, ondansetron (OND), at the Vc/C1–2 region reduced the ATP-evoked responses of neurons in superficial and deep laminae and reduced the EMG response in both sham and FS rats. OND also decreased the spontaneous firing rate of neurons in deep laminae and reduced the high threshold convergent cutaneous receptive field area of neurons in superficial and deep laminae in both sham and FS rats. These results revealed that central application of a 5HT3R antagonist, had widespread effects on the properties of TMJ-responsive neurons at the Vc/C1–2 region and on jaw muscle reflexes under sham and FS conditions. It is concluded that 5HT3R does not play a unique role in mediating stress-induced hyperalgesia related to TMJ nociception.

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“…This modulation is realized by three medullar areas reciprocally connected with the dorsal horn: the rostroventral medial medulla (RVM), the VLM and the dorsal reticular nucleus (DRt) via noradrenergic and serotonergic descending pathways (Tavares and Lima, 2002;Heinricher et al, 2009). Several studies in various pain models have demonstrated that the 5-HT 3 receptors mediate the facilitation of the spinal cord pain transmission (Rahman et al, , 2009Vera-Portocarrero et al, 2006;Sikandar et al, 2012), which is performed through direct serotonergic projections from the RVM to the spinal cord by activation of the spinal 5-HT 3 receptors located at pre-synaptic sites on the terminals of glutamate-releasing primary afferent fibers, as well as on excitatory interneurons Conte et al, 2005). The blockade of these receptors with granisetron appears to result in a decrease in primary spinal neuron responses to noxious CRD and an inhibition of ascending nociceptive signals to the brainstem.…”

Section: Discussionmentioning

confidence: 99%

The inhibitory effect of granisetron on ventrolateral medulla neuron responses to colorectal distension in rats

Panteleev

Martseva

Lyubashina

2015

European Journal of Pharmacology

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Transmitter content, origins and connections of axons in the spinal cord that possess the serotonin (5-hydroxytryptamine) 3 receptor

Cited by 44 publications

References 32 publications

Presynaptic Inhibitory Effects of Fluvoxamine, a Selective Serotonin Reuptake Inhibitor, on Nociceptive Excitatory Synaptic Transmission in Spinal Superficial Dorsal Horn Neurons of Adult Mice

Presynaptic Inhibitory Effects of Fluvoxamine, a Selective Serotonin Reuptake Inhibitor, on Nociceptive Excitatory Synaptic Transmission in Spinal Superficial Dorsal Horn Neurons of Adult Mice

Inhibition of temporomandibular joint input to medullary dorsal horn neurons by 5HT3 receptor antagonist in female rats

The inhibitory effect of granisetron on ventrolateral medulla neuron responses to colorectal distension in rats

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