Transmission of Xanthomonas Campestris Pv. Campestris in Seed Production Crops of Cauliflower

Kastelein, P.; Krijger, M.C.; Zouwen, P.S. van der; Steen, J.J.M. van der; Stevens, Lucas H.; Wolf, J.M. van der; Vieira, Jucilayne Fernandes; Villela, Francisco Amaral

doi:10.17660/actahortic.2014.1041.22

Cited by 3 publications

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“…Brassica is a cross-pollinator and the use of pollen from a crop in which Xcc is present bears a risk for carry-over contamination to plants and in particular a risk for pollen-to ovule transfer resulting in seed infections. Flower inoculations with Xcc have been shown to result relatively easy in seed infections , Kastelein et al, 2014.…”

Section: Discussionmentioning

confidence: 99%

Mapping tracks of Xanthomonas campestris pv. campestris resulting in Brassica seed infections

Wolf

Kastelein

Poleij

et al. 2020

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Section: Discussionmentioning

confidence: 99%

Mapping tracks of Xanthomonas campestris pv. campestris resulting in Brassica seed infections

Wolf

Kastelein

Poleij

et al. 2020

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“…In practice, seed infection incidences of 0.01% are tolerated for direct seeded field crops, whereas for transplanted crops an even lower infection incidence can cause economic damage (Schaad et al 1980;Roberts et al 2007). Black rot due to the use of seed infected by Xcc can be influenced by various factors such as environmental conditions (Ignatov et al 1999a;Kastelein et al 2014), the bacterial strain (Guy et al 2013;Chidamba and Bezuidenhout 2012;Kastelein et al 2014), the genotype used (Ignatov et al 1998(Ignatov et al , 1999bvan der Wolf et al 2013), as well as pathogen densities. Seeds vacuum-infiltrated with Xcc suspensions as low as 1.4 cfu per seed were able to cause 1-4% black rot diseased plants (Roberts et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Colonization of siliques and seeds of rapid cycling Brassica oleracea plants by Xanthomonas campestris pv. campestris after spray-inoculation of flower clusters

et al. 2019

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Colonization of siliques and seeds of rapid cycling Brassica oleracea plants by Xanthomonas campestris pv. campestris after spray-inoculation of flower clusters Abstract Glasshouse experiments were conducted to study the colonization of seedpods (siliques) and seeds of rapid cycling Brassica oleracea plants after spraying inoculum on clusters of recently opened flowers with Xanthomonas campestris pv. campestris (Xcc) at densities of 10 7 -10 8 cfu ml −1 . A green fluorescent protein (GFP) tagged Xcc strain was used to allow visualization of the bacteria by epifluorescence stereo microscopy (ESM) and confocal laser scanning microscopy (CLSM). The GFP-tagged strain showed reduced virulence compared to the untagged parental strain, but was still able to cause black rot symptoms. Two to three days after sprayinoculation, sepals, stamen and petals were colonized by Xcc, as observed by ESM. In green siliques a GFP-signal was observed on valves, septa and seeds, despite the fact that a high percentage of Xcc cells had lost their ability to express GFP as found by dilution-plating. Densities of Xcc in infected silique tissues were up to 10 9 cfu g −1 . A fluorescent signal using ESM was found in seeds harvested from symptomatic siliques after incubation of seeds on blotting paper wetted with broth to enhance the multiplication of Xcc. Xcc was found in association with the seed coat and in a single seed, also in the endosperm and embryo, indicating deep-seated seed infection. The estimated incidence of contaminated seeds in both years was ca. 7%. The estimated incidence of deep-seated infections, still detectable after warm water treatment of seeds, was also high (2-3.8%). It is concluded that spray-inoculation of flower clusters with Xcc can result in the infection of sepals and reproductive organs, and in deep-seated seed infections.

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Beehold : the colony of the honeybee (Apis mellifera L) as a bio-sampler for pollutants and plant pathogens

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Transmission of Xanthomonas Campestris Pv. Campestris in Seed Production Crops of Cauliflower

Cited by 3 publications

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Mapping tracks of Xanthomonas campestris pv. campestris resulting in Brassica seed infections

Mapping tracks of Xanthomonas campestris pv. campestris resulting in Brassica seed infections

Colonization of siliques and seeds of rapid cycling Brassica oleracea plants by Xanthomonas campestris pv. campestris after spray-inoculation of flower clusters

Beehold : the colony of the honeybee (Apis mellifera L) as a bio-sampler for pollutants and plant pathogens

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