Transmission of human cytomegalovirus via breastmilk and potential risks to very preterm infants

Hamprecht, Klaus; Goelz, Rangmar

doi:10.1071/ma15066

Cited by 1 publication

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“…Only one seropositive mother failed to yield any evidence for viral reactivation during the observation period. This is not unusual, because it has been reported that not exactly 100% of seropositive breastfeeding mothers shed the virus into milk (9,12,30).…”

Section: Discussionmentioning

confidence: 93%

Immunomonitoring of Human Breast Milk Cells During HCMV-Reactivation

et al. 2021

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BackgroundBreast milk leukocytes may play a role in protecting the infant from pathogens. The dynamics and the role of lymphocytes in human cytomegalovirus (HCMV)-seropositive mothers shedding HCMV into breast milk during the first months postpartum (p.p.) are mostly unclear.MethodsBreast milk cells were analyzed by Pappenheim panoptic and alpha-naphthyl acetate esterase staining as well as by imaging and polychromatic flow cytometry to simultaneously establish their morphological and phenotypic properties. The latter were characterized in HCMV-seropositive and seronegative mothers´ breast milk cells at different time points p.p.ResultsPanoptic staining of breast milk cells revealed the presence of monocytes/macrophages, granulocytes and lymphocytes. Imaging flow cytometry data combining phenotypic and morphological analysis identified NKT-like cells, NK cells, epithelial cells, T cells and monocytes/macrophages. HCMV-seropositive but not -seronegative mothers had significantly higher T cell frequencies in mature milk.ConclusionsThe presence of lymphocyte subsets in breast milk may be more influenced by the HCMV-seropositivity of the mother than previously recognized.

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Section: Discussionmentioning

confidence: 93%