Transmission of bovine viral diarrhea virus (BVDV) via in vitro-fertilized embryos to recipients, but not to their offspring

Bielański, A.; Algire, J.; Lalonde, A.; Nadin-Davis, Susan A.

doi:10.1016/j.theriogenology.2008.08.015

Cited by 38 publications

(9 citation statements)

References 31 publications

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“…In fact, the most frequently used sources for cryopreservation are animal-derived products, such as foetal calf serum (FCS) or bovine serum albumin (BSA), which may be contaminated by pathogens, particularly by viruses [4], [6], [7]. Among these viruses, Bovine Viral Diarrhoea virus is the most important cause of concern because of its large economic impact [8], its prevalence in FCS [9], its relative resistance to gamma irradiation [6] and cryopreservation procedures [10], and consequently the associated risk of disease transmission to recipients by an infected embryo [11]. Prion diseases can also probably be transmitted through blood-derived products [12]–[14], especially as it is difficult to inactivate the transmissible degenerative encephalopathy agents with the usual processing of animal-based products [15].…”

Section: Introductionmentioning

confidence: 99%

A Chemically Defined Medium for Rabbit Embryo Cryopreservation

Bruyère¹,

Baudot²,

Joly³

et al. 2013

PLoS ONE

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This study evaluates a new synthetic substitute (CRYO3, Ref. 5617, Stem Alpha, France) for animal-based products in rabbit embryo cryopreservation solutions. This evaluation was performed using two approaches: a thermodynamic approach using differential scanning calorimetry and a biological approach using rabbit embryo slow-freezing. During the experiment, foetal calf serum (FCS) was used as a reference. Because FCS varies widely by supplier, three different FCS were selected for the thermodynamic approach. The rabbit embryo slow-freezing solutions were made from Dulbecco's phosphate buffer saline containing 1.5 M Dimethyl Sulfoxide and 18% (v.v−1) of CRYO3 or 18% (v.v−1) of FCS. These solutions were evaluated using four characteristics: the end of melting temperature, the enthalpy of crystallisation (thermodynamic approach) and the embryo survival rates after culture and embryo transfer (biological approach). In the thermodynamic approach, the solutions containing one of the three different FCS had similar mean thermodynamic characteristics but had different variabilities in the overall data with aberrant values. The solution containing CRYO3 had similar thermodynamic properties when compared to those containing FCS. Moreover, no aberrant value was measured in the solution containing CRYO3. This solution appears to be more stable than the solutions containing a FCS. In the biological approach, the in vitro embryo survival rates obtained with the solution containing CRYO3 (73.7% and 81.3%) and with the solution containing a FCS (77.6% and 71.9%) were similar (p = 0.7). Nevertheless, during the in vivo evaluation, the implantation rate (21.8%) and the live-foetuses rate (18.8%) of the CRYO3 group were significantly higher than the implantation rate (7.1%, p = 0.0002) and the live-foetuses rate (5.3%, p = 0.0002) of the FCS group. The pregnancy rate was also higher in the CRYO3 group compared to the FCS group (81.3% and 43.8%, respectively, p = 0.066). We conclude that CRYO3 can be used as a chemically defined substitute for animal-based products in rabbit embryo cryopreservation solutions.

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Section: Introductionmentioning

confidence: 99%

A Chemically Defined Medium for Rabbit Embryo Cryopreservation

Bruyère¹,

Baudot²,

Joly³

et al. 2013

PLoS ONE

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“…To optimize the procedures, the tissues were macerated in the respective lysis buffers using sterilized microtube pestels. Detection of GAPDH, as described by Oliveira et al (2013), and ACTB (β-actin), as described by Bielanski et al (2009), was used to confirm the DNA extraction efficiency.…”

Section: Methodsmentioning

confidence: 99%

Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems

Bicalho

Júnior

Oliveira

et al. 2016

SCA

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Reproductive performance of dairy cows has a direct impact on herd productivity. Infectious agents, such as Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV), are associated with reproductive failure. However, it remains unknown if these viruses are present in the uterus and cause gestational loss. This study used molecular methods to assess if BTV and BLV can be detected in the uterus of serologically positive dairy cows with a record of abortions, stillbirths and repeat breeding (n=23) and without a record of reproductive problems (n =23). The cows came from three dairy herds of the state of Minas Gerais, Brazil. BTV was not detected in any of the uterine biopsies. Proviral DNA of BLV was detected in 54.5 % of the seropositive cows, but positivity for BLV in the uterus was not associated with the existence of reproductive problems. In conclusion, this study shows that BLV, but not BTV, is present in the uterus of seropositive cows, regardless of reproductive performance. Key words: Bovines. Reproduction. Uterus. BTV. BLV. associados à ocorrência de falhas reprodutivas. Entretanto, não se sabe se estes vírus poderiam estar presentes no útero resultando nas perdas gestacionais. Este estudo utilizou métodos moleculares para investigar se os vírus BTV e BLV estão presentes no úterio de vacas leiteiras sorologicamente positivas com histórico de abortos, natimortos e repetição de cio (n=23) e em vacas sem histórico de problema reprodutivos (n =23) provenientes de três rebanhos leiteiros localizados no estado de Minas Gerais, Brasil. Não foi detectado BTV em nenhuma amostra de biópsia uterina. Por outro lado, o DNA proviral de BLV foi detectado no útero de 54.5 % das vacas soropositivas, independentemente da ocorrência de problemas reprodutivos. Conclui-se que o vírus BLV, ao contrário do BTV está presente no útero de vacas soropositivas, entretanto, sem associação com o desempenho reprodutivo. Palavras-chave: Bovinos. Reprodução. Útero. BTV. BLV. Resumo

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“…Samples were subjected to a RT-qPCR to the normalizing gene beta-actin, following the protocol described by Bielanski et al [15] in order to verify the efficiency of RNA extraction and the presence of inhibitors.…”

Section: Materials Studied Area Descriptions Methods and Techniquesmentioning

confidence: 99%

Validation of a Real Time PCR for Classical Swine Fever Diagnosis

Dias

Fonseca

Oliveira

et al. 2014

Veterinary Medicine International

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The viral disease classical swine fever (CSF), caused by a Pestivirus, is one of the major causes of economic losses for pig farming. The aim of this work was to validate a RT-qPCR using Taqman for detection of CSF in swine tissues. The parameters for the validation followed the specifications of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for Animal Health (OIE) and the guide ABNT NBR ISO/IEC 17025:2005. The analysis of the 5′NTR region of CSF virus was performed in 145 samples from 29 infected pigs and in 240 samples from 80 pigs originated in the Brazilian CSF-free zone. The tissues tested were spleen, kidney, blood, tonsils, and lymph nodes. Sequencing of the positive samples for 5′NTR region was performed to evaluate the specificity of the RT-qPCR. Tests performed for the RT-qPCR validation demonstrated that the PCR assay was efficient in detecting RNA from CSF virus in all materials from different tissues of infected animals. Furthermore, RNA from CSF virus was not detected in samples of swine originated from the Brazilian CSF-free zone. Hence, it is concluded that RT-qPCR can be used as a complementary diagnostic for CSF.

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Transmission of bovine viral diarrhea virus (BVDV) via in vitro-fertilized embryos to recipients, but not to their offspring

Cited by 38 publications

References 31 publications

A Chemically Defined Medium for Rabbit Embryo Cryopreservation

A Chemically Defined Medium for Rabbit Embryo Cryopreservation

Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems

Validation of a Real Time PCR for Classical Swine Fever Diagnosis

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