Transmission of antimicrobial resistant non-O157 Escherichia coli at the interface of animal-fresh produce in sustainable farming environments

Glaize, Ayanna; Gutiérrez-Rodríguez, Eduardo; Hanning, Irene; Díaz-Sánchez, Sandra; Gunter, Chris; Vliet, Arnoud H. M. van; Watson, Wes; Thakur, Siddhartha

doi:10.1016/j.ijfoodmicro.2019.108472

Cited by 15 publications

(21 citation statements)

References 53 publications

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“…Because animals may shed enteric zoonotic organisms in their feces, good agricultural practices often include grower assessments of potential animal-derived contamination in areas where their crop is grown and harvested ( 25 – 27 ). The movement of dusts and bioaerosols containing microorganisms from animal operations to nearby plant crops represents one possible transfer route; however, this mechanism of transfer is not well characterized ( 8 , 16 , 28 ), making it difficult for growers to appropriately assess their risk. The current study provides evidence that microorganisms moved a short distance into an almond orchard from a poultry operation that was upwind and 35 m from end of the barns to the first row of trees based on (i) the higher prevalence of E. coli in AP air and soil, (ii) the higher levels of dust found in AP at 0 m into the orchard, and (iii) altered phyllosphere microbiota (higher prevalence of the bacterial family Staphylococcaceae ) found in the phyllosphere in AP at 0 and 60 m into the orchard.…”

Section: Discussionmentioning

confidence: 99%

“…Epidemiological evidence has linked cattle to outbreaks of foodborne illness associated with leafy greens grown nearby, with irrigation or other agricultural water uses or wildlife potentially serving as an intermediary ( 2 – 6 ). Birds and insects have the potential to move pathogens from animal operations into growing environments ( 7 – 12 ). Bioaerosols capable of carrying pathogenic bacteria have been described to occur in cattle feedlots, in poultry and swine houses, and during field application of manure ( 13 – 18 ).…”

Section: Introductionmentioning

confidence: 99%

“… E. coli O157:H7 could be recovered from 3.5%, 2.2%, and 1.8% of leafy green samples collected over a 4-month period at 60, 120, and 180 m, respectively, from a cattle feedlot; airborne transport of the pathogen was implicated, and pen dryness and animal activities that generate airborne dust were the noted risk factors ( 13 ). Transfer of pathogenic E. coli from livestock to vegetable production systems may be influenced by both the size of the animal operation and proximity to the farm ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

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Microorganisms Move a Short Distance into an Almond Orchard from an Adjacent Upwind Poultry Operation

Theofel

Williams

Gutierrez

et al. 2020

Appl Environ Microbiol

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Over a 2-year period, drag swabs of orchard soil surface and air, soil, and almond leaf samples were collected in an almond orchard adjacent to (35 m from the first row of trees) and downwind from a poultry operation and in two almond orchards (controls) that were surrounded by other orchards. Samples were evaluated for aerobic plate count, generic Escherichia coli, other coliforms, the presence of Salmonella, bacterial community structure (analyzed through sequencing of the 16S rRNA gene), and amounts of dry solids (dust) on leaf surfaces on trees 0, 60, and 120 m into each orchard. E. coli was isolated from 41 of 206 (20%) and 1 of 207 (0.48%) air samples in the almond-poultry and control orchards, respectively. Salmonella was not isolated from any of the 529 samples evaluated. On average, the amount of dry solids on leaves collected from trees closest to the poultry operation was more than 2-fold greater than from trees 120 m into the orchard or from any of the trees in the control orchards. Members of the family Staphylococcaceae—often associated with poultry—were, on average, significantly (P < 0.001) more abundant in the phyllosphere of trees closest to the poultry operation (10% of relative abundance) than in trees 120 m into the orchard (1.7% relative abundance) or from any of the trees in control orchards (0.41% relative abundance). Poultry-associated microorganisms from a commercial operation transferred a short distance into an adjacent downwind almond orchard. IMPORTANCE The movement of microorganisms, including foodborne pathogens, from animal operations into adjacent plant crop-growing environments is not well characterized. This study provides evidence that dust and bioaerosols moved from a commercial poultry operation a short distance downwind into an almond orchard and altered the microbiome recovered from the leaves. These data provide growers with information they can use to assess food safety risks on their property.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microorganisms Move a Short Distance into an Almond Orchard from an Adjacent Upwind Poultry Operation

Theofel

Williams

Gutierrez

et al. 2020

Appl Environ Microbiol

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“…It can be assumed that meat introduced in the processing facilities acts as primary source of AMR (Arg € uello et al, 2013a), which then can be indirectly spread in meat processing. Despite the presence of food-borne pathogens, which is well documented (Giovannacci et al, 2001;Arg € uello et al, 2013b), the carriage of AMR is poorly investigated. Sala et al ( 2016) conducted a study in a Romanian processing plant, where they evaluated the presence of L. monocytogenes.…”

Section: E1322 Processing Plantsmentioning

confidence: 99%

“…The frequent isolation of resistant and multi-resistant isolates from carcasses (Bolton et al, 2013;Garc ıa-Fierro et al, 2016;Fois et al, 2017) would suggest that similar resistant profiles can be found in the slaughterhouse environment (machinery, workers hands and implements, etc. ), and there is a connection between Salmonella present on carcasses and slaughter activities (Giovannacci et al, 2001;Arg € uello et al, 2013a;Gomes-Neves et al, 2014). Two studies describe the isolation of MRSA resistant to gentamicin in the slaughterhouse environment (Van Cleef et al, 2010) and particularly in workers involved in scalding and de-hairing activities (Gilbert et al, 2012).…”

Section: F232 Pigs Post-harvest: Arb and Argsmentioning

confidence: 99%

Role played by the environment in the emergence and spread of antimicrobial resistance (AMR) through the food chain

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2021

EFS2

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The role of food-producing environments in the emergence and spread of antimicrobial resistance (AMR) in EU plant-based food production, terrestrial animals (poultry, cattle and pigs) and aquaculture was assessed. Among the various sources and transmission routes identified, fertilisers of faecal origin, irrigation and surface water for plant-based food and water for aquaculture were considered of major importance. For terrestrial animal production, potential sources consist of feed, humans, water, air/dust, soil, wildlife, rodents, arthropods and equipment. Among those, evidence was found for introduction with feed and humans, for the other sources, the importance could not be assessed. Several ARB of highest priority for public health, such as carbapenem or extended-spectrum cephalosporin and/or fluoroquinolone-resistant Enterobacterales (including Salmonella enterica), fluoroquinolone-resistant Campylobacter spp., methicillin-resistant Staphylococcus aureus and glycopeptide-resistant Enterococcus faecium and E. faecalis were identified. Among highest priority ARGs bla CTX-M , bla VIM , bla NDM , bla OXA-48like , bla OXA-23 , mcr, armA, vanA, cfr and optrA were reported. These highest priority bacteria and genes were identified in different sources, at primary and post-harvest level, particularly faeces/manure, soil and water. For all sectors, reducing the occurrence of faecal microbial contamination of fertilisers, water, feed and the production environment and minimising persistence/recycling of ARB within animal production facilities is a priority. Proper implementation of good hygiene practices, biosecurity and food safety management systems is very important. Potential AMR-specific interventions are in the early stages of development. Many data gaps relating to sources and relevance of transmission routes, diversity of ARB and ARGs, effectiveness of mitigation measures were identified. Representative epidemiological and attribution studies on AMR and its effective control in food production environments at EU level, linked to One Health and environmental initiatives, are urgently required.

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Multistate outbreak of Salmonella Oranienburg infections linked to bulb onions imported from Mexico – United States, 2021

Mitchell,

Kirchner,

Schneider

et al. 2024

Food Control

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Transmission of antimicrobial resistant non-O157 Escherichia coli at the interface of animal-fresh produce in sustainable farming environments

Cited by 15 publications

References 53 publications

Microorganisms Move a Short Distance into an Almond Orchard from an Adjacent Upwind Poultry Operation

Microorganisms Move a Short Distance into an Almond Orchard from an Adjacent Upwind Poultry Operation

Role played by the environment in the emergence and spread of antimicrobial resistance (AMR) through the food chain

Multistate outbreak of Salmonella Oranienburg infections linked to bulb onions imported from Mexico – United States, 2021

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